1 resultado para Problem children - Behavior modification
em AMS Tesi di Dottorato - Alm@DL - Università di Bologna
Filtro por publicador
- Aberystwyth University Repository - Reino Unido (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (1)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (2)
- Aquatic Commons (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (4)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (16)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (21)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (2)
- Bioline International (9)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (30)
- Brock University, Canada (20)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (4)
- CaltechTHESIS (8)
- Cambridge University Engineering Department Publications Database (4)
- CentAUR: Central Archive University of Reading - UK (23)
- Central European University - Research Support Scheme (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (21)
- Cochin University of Science & Technology (CUSAT), India (4)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Cornell: DigitalCommons@ILR (1)
- Dalarna University College Electronic Archive (4)
- Deakin Research Online - Australia (145)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons - Michigan Tech (2)
- Digital Commons @ DU | University of Denver Research (11)
- Digital Commons at Florida International University (31)
- DigitalCommons - The University of Maine Research (2)
- DigitalCommons@The Texas Medical Center (43)
- DigitalCommons@University of Nebraska - Lincoln (2)
- DRUM (Digital Repository at the University of Maryland) (5)
- Duke University (12)
- Ecology and Society (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Helda - Digital Repository of University of Helsinki (9)
- Indian Institute of Science - Bangalore - Índia (19)
- Institute of Public Health in Ireland, Ireland (1)
- Instituto Politécnico de Santarém (2)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (2)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (1)
- Massachusetts Institute of Technology (5)
- Memoria Académica - FaHCE, UNLP - Argentina (6)
- Ministerio de Cultura, Spain (3)
- National Center for Biotechnology Information - NCBI (5)
- Open University Netherlands (1)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Publishing Network for Geoscientific & Environmental Data (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (52)
- Queensland University of Technology - ePrints Archive (136)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (3)
- REPOSITÓRIO ABERTO do Instituto Superior Miguel Torga - Portugal (1)
- Repositorio Académico de la Universidad Nacional de Costa Rica (1)
- Repositório Científico da Escola Superior de Enfermagem de Coimbra (1)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (3)
- Repositório Científico do Instituto Politécnico de Santarém - Portugal (2)
- Repositório digital da Fundação Getúlio Vargas - FGV (5)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (87)
- Research Open Access Repository of the University of East London. (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (4)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (2)
- School of Medicine, Washington University, United States (3)
- Scielo España (1)
- Scielo Uruguai (1)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (17)
- Universidad Politécnica de Madrid (16)
- Universidade Federal do Pará (4)
- Universidade Federal do Rio Grande do Norte (UFRN) (2)
- Universidade Técnica de Lisboa (1)
- Universita di Parma (1)
- Universitat de Girona, Spain (4)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (5)
- Université de Lausanne, Switzerland (3)
- Université de Montréal (3)
- Université de Montréal, Canada (14)
- University of Canberra Research Repository - Australia (1)
- University of Connecticut - USA (3)
- University of Michigan (23)
- University of Queensland eSpace - Australia (35)
- University of Washington (9)
- WestminsterResearch - UK (2)
Resumo:
Protein-adsorption occurs immediately following implantation of biomaterials. It is unknown at which extent protein-adsorption impacts the cellular events at bone-implant interface. To investigate this question, we compared the in-vitro outcome of osteoblastic cells grown onto titanium substrates and glass as control, by modulating the exposure to serum-derived proteins. Substrates consisted of 1) polished titanium disks; 2) polished disks nanotextured with H2SO4/H2O2; 3) glass. In the pre-adsorption phase, substrates were treated for 1h with αMEM alone (M-noFBS) or supplemented with 10%-foetal-bovine-serum (M-FBS). MC3T3-osteoblastic-cells were cultured on the pre-treated substrates for 3h and 24h, in M-noFBS and M-FBS. Subsequently, the culture medium was replaced with M-FBS and cultures maintained for 3 and 7days. Cell-number was evaluated by: Alamar-Blue and MTT assay. Mitotic- and osteogenic-activities were evaluated through fluorescence-optical-microscope by immunolabeling for Ki-67 nuclear-protein and Osteopontin. Cellular morphology was evaluated by SEM-imaging. Data were statistically analyzed using ANOVA-test, (p<0.05). At day3 and day7, the presence or absence of serum-derived proteins during the pre-adsorption phase had not significant effect on cell-number. Only the absence of FBS during 24h of culture significantly affected cell-number (p<0.0001). Titanium surfaces performed better than glass, (p<0.01). The growth rate of cells between day3 and 7 was not affected by the initial absence of FBS. Immunolabeling for Ki-67 and Osteopontin showed that the mitotic- and osteogenic- activity were ongoing at 72h. SEM-analysis revealed that the absence of FBS had no major influence on cell-shape. • Physico-chemical interactions without mediation by proteins are sufficient to sustain the initial phase of culture and guide osteogenic-cells toward differentiation. • The challenge is avoiding adsorption of ‘undesirables’ molecules that negatively impact on the cueing cells receive from surface. This may not be a problem in healthy patients, but may have an important role in medically-compromised-individuals in whom the composition of tissue-fluids is altered.