Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy

[EN] Background: Either higher levels of initial DNA damage or lower levels of radiation-induced apoptosis in peripheral blood lymphocytes have been associated to increased risk for develop late radiation-induced toxicity. It has been recently published that these two predictive tests are inversely related. The aim of the present study was to investigate the combined role of both tests in relation to clinical radiation-induced toxicity in a set of breast cancer patients treated with high dose hyperfractionated radical radiotherapy. Methods: Peripheral blood lymphocytes were taken from 26 consecutive patients with locally advanced breast carcinoma treated with high-dose hyperfractioned radical radiotherapy. Acute and late cutaneous and subcutaneous toxicity was evaluated using the Radiation Therapy Oncology Group morbidity scoring schema. The mean follow-up of survivors (n = 13) was 197.23 months. Radiosensitivity of lymphocytes was quantified as the initial number of DNA double-strand breaks induced per Gy and per DNA unit (200 Mbp). Radiation-induced apoptosis (RIA) at 1, 2 and 8 Gy was measured by flow cytometry using annexin V/propidium iodide. Results: Mean DSB/Gy/DNA unit obtained was 1.70 ± 0.83 (range 0.63-4.08; median, 1.46). Radiation-induced apoptosis increased with radiation dose (median 12.36, 17.79 and 24.83 for 1, 2, and 8 Gy respectively). We observed that those "expected resistant patients" (DSB values lower than 1.78 DSB/Gy per 200 Mbp and RIA values over 9.58, 14.40 or 24.83 for 1, 2 and 8 Gy respectively) were at low risk of suffer severe subcutaneous late toxicity (HR 0.223, 95%CI 0.073-0.678, P = 0.008; HR 0.206, 95%CI 0.063-0.677, P = 0.009; HR 0.239, 95%CI 0.062-0.929, P = 0.039, for RIA at 1, 2 and 8 Gy respectively) in multivariate analysis. Conclusions: A radiation-resistant profile is proposed, where those patients who presented lower levels of initial DNA damage and higher levels of radiation induced apoptosis were at low risk of suffer severe subcutaneous late toxicity after clinical treatment at high radiation doses in our series. However, due to the small sample size, other prospective studies with higher number of patients are needed to validate these results.

Uso de textos musicales (canciones) en la enseñanza del inglés

[ES]El presente artículo de investigación tiene como objetivo principal evaluar cómo el uso de los textos musicales –canciones, como popularmente se conocen– influye en el aprendizaje del inglés como lengua extranjera. Una investigación llevada a cabo con estudiantes de una clase de 2º de ESO nos ha servido para confirmar las hipótesis que en un principio teníamos en lo concerniente a la adquisición de las ocho competencias básicas mediante tareas en las clases de lengua inglesa. Así, el diseño de rúbricas con unos criterios e instrumentos de evaluación específicos nos ha permitido evaluar el grado de adquisición de competencias básicas concretas. [EN]This paper focuses on the impact that the use of music texts –commonly referred to with the term ‘songs’– has on students in teaching English as a foreign language. Through a survey carried out with students of a 2nd year class of Spanish Secondary Education, our initial assumptions in terms of acquisition of key competences in English learning were validated. in acquiring specific key competences by using tasks, rubrics –consisting of detailed assessment criteria and tools– were designed and implemented as the assessment method in order for students to successfully attain specific learning objectives.