Biodiversidad química del litoral canario

Programa de doctorado de Oceanografía, Bienio 2003-2005. La fecha de publicación es la fecha de lectura

Variación del perfil polifenólico de diatomeas Phaeodactylum tricornutum cultivadas en agua de mar enriquecida en cobre (II)

[ES]Los polifenoles se caracterizan por su capacidad antioxidante. En este trabajo se ha desarrollado un método para determinar el perfil fenólico de extractos de diatomea de la especie Phaeodactylum tricornutum. Dicho método combina la preconcentración mediante SPE y HPLC para la identificación y cuantificación de los polifenoles. El estudio de extractos de ésta diatomea cultivada en agua de mar enriquecida con cobre evidencia una acumulación de compuestos fenólicos inducida por las altas concentraciones del metal. Si comparamos las concentraciones de polifenoles con las obtenidas para un blanco, éstas aumentan cuando aumenta la concentración de cobre.

Estimulación de la producción de compuestos polifenólicos en microalgas cultivadas en condiciones de estrés oxidativo por altas concentraciones de hierro (III) y cobre (II)

[EN] The study investigates the phenolic profile of exudates and extracts of several microalgae harvested in natural seawater in the presence and absence of Cu and Fe additions, at metal levels of environmental relevance. The experimental findings show changes in the concentrations and types of polyphenols as a function of the metal added and its spiked concentrations. The results shown might be useful to help explaining the dynamics of this important class of compounds in seawater and also encourage further studies aimed at using algae as a source of chemical principles to be used in the health, food and pharmaceutical industry.

Extraction system and determination of PAH in seawater and it implementation in submarine vehicles

[EN]Policyclyc aromatic hydrocarbons (PAHs) are a potential risk for human health and marine biota in general that make necessary the monitorization of them. A miniaturized extraction system capable to extract PAHs from seawater was developed and optimized with the objective of implement it in an oceanographic buoy in the future. An analytical method was optimized by high performance liquid chromatography for the determination of extracted PAHs by the extraction system. The analytical method was validated and applicated to real samples of differents points of Gran Canaria. The method has enough sensitivity to detect and quantify concentrations below the concentrations established in the legislation. In some places where samples were taken some compounds exceed the legislation while other compounds follow it