Molecular analysis of three FUT3 gene single nucleotide polymorphisms and their relationship with the lewis erythrocytary phenotype in a human population of japanese-ancestry living in Tomé Açu, a town in the Brazilian Amazon

The Lewis blood group system involves two major antigens, Le^ª and Le^b. Their antigenic determinants are not primary gene products but are synthesized by the transfer of sugar subunits to a precursory chain by a specific enzyme which is the product of the FUT3 gene (Lewis gene). The presence of three FUT3 gene single nucleotide polymorphisms (SNPs) (59T > G; 508G > A and 1067T > A) was related to the Lewis phenotype of erythrocytes from 185 individuals of Japanese ancestry living in the town of Tomé-Açu in the Brazilian Amazon region. This relationship was detected using a serological hemagglutination test and the Dot-ELISA assay along with the molecular technique PCR-RFLP. We found that the three SNPs investigated in this study only accounted for a proportion of the Lewis-negative phenotype of the erythrocytes.

Os direitos humanos de acessibilidade e locomoção das pessoas com necessidades especiais: a realidade paraense, com ênfase em Belém/PA

Aborda a problemática enfrentada, diuturnamente, pelas pessoas com necessidades especiais, frente às barreiras arquitetônicas, nos edifícios destinados ao uso público e nos meios de transporte coletivo, bem como diante da falta de equipamentos e elementos do mobiliário urbano acessíveis. Utiliza dados censitários acerca das referidas pessoas. Analisa essa problemática sob o enfoque dos direitos humanos. Enfatiza a imprescindibilidade de eliminação das referidas barreiras ou de serem adaptadas. Demonstra como o assunto é tratado no plano internacional e como está substanciado em normas constitucionais nacionais e estaduais, nas leis orgânicas municipais e na legislação infraconstitucional específica nos níveis federal, estadual e municipal, bem assim em normas técnicas. Aponta a precariedade das estruturas físico-ambientais, principalmente no município de Belém, Estado do Pará. Contém propostas para a efetividade das normas de acessibilidade e locomoção das aludidas pessoas e a concreção desses direitos humanos.

Molecular analysis of three FUT3 gene single nucleotide polymorphisms and their relationship with the lewis erythrocytary phenotype in a human population of japanese-ancestry living in Tomé Açu, a town in the Brazilian Amazon

The Lewis blood group system involves two major antigens, Le^a and Le^b. Their antigenic determinants are not primary gene products but are synthesized by the transfer of sugar subunits to a precursory chain by a specific enzyme which is the product of the FUT3 gene (Lewis gene). The presence of three FUT3 gene single nucleotide polymorphisms (SNPs) (59T > G; 508G > A and 1067T > A) was related to the Lewis phenotype of erythrocytes from 185 individuals of Japanese ancestry living in the town of Tomé-Açu in the Brazilian Amazon region. This relationship was detected using a serological hemagglutination test and the Dot-ELISA assay along with the molecular technique PCR-RFLP. We found that the three SNPs investigated in this study only accounted for a proportion of the Lewis-negative phenotype of the erythrocytes.