Projeto e implementação de um nodo sensor para aquisição de sinais de extensômetros em redes de sensores sem fio aplicado ao monitoramento de deformações em estruturas

Esta dissertação tem como objetivo principal propor um nodo (ou nó) sensor sem fio para ser utilizado em redes de sensores sem fio, em sistemas de aquisição de dados de extensômetros. O sistema de aquisição para os extensômetros é baseado na ponte de Wheatstone e de modo a permitir várias configurações de extensômetros. O processamento e a comunicação sem fio é realizada pelo ATmega128RFA1, composto por um microcontrolador e um transceiver Rádio-Frequência com o padrão Zigbee. O nodo foi projetado para garantir confiabilidade na aquisição de dados e ser totalmente controlado remotamente. Entre os parâmetros controláveis estão: o ganho do sinal e a taxa de amostragem. Além disso, o nodo possui recursos para efetuar o equilíbrio da ponte de Wheatstone automaticamente. A escolha de seus componentes, baseou-se em critérios relacionados ao consumo de energia do mesmo e ao custo. Foi concebida uma placa de circuito impresso (PCI) para o nodo, e sobre ela foram realizadas estimativas sobre o consumo de energia e valor agregado do protótipo, com o objetivo de analisar a sua viabilidade. Além do projeto do nodo sensor, o trabalho apresenta a proposta de integração do mesmo em uma rede de sensores sem fio (RSSF), incluindo a sugestão do hardware complementar e desenvolvimentos dos softwares. Para os testes do nodo sensor, foi construido experimentalmente um transdutor de força.

Modulation of nociceptive-like behavior in zebrafish (Danio rerio) by environmental stressors

Zebrafish have been demonstrated to react consistently to noxious chemical stimuli and present reliable phenotypes of stress, fear, and anxiety. In this article, we describe the modulation of nociceptive-like responses of zebrafish to fear-, stress-, and anxiety-eliciting situations. Animals were exposed to an alarm substance, confinement stress, or a novel environment before being injected with 1% acetic acid in the tail. The alarm substance and confinement stress reduced the display of erratic movements and tail-beating behavior elicited by acetic acid. The novelty of the environment, in contrast, increased the frequency of tail-beating behavior. The results suggest that descending modulatory control of nociception exists in zebrafish, with apparent fear- and stress-induced analgesia and anxiety-induced hyperalgesia.

HLA-Bw4-B*57 and Cw*18 alleles are associated with plasma viral load modulation in HIV-1 infected individuals in Salvador, Brazil

Host genetic factors play an important role in mediating resistance to HIV-1 infection and may modify the course of infection. HLA-B alleles (Bw4 epitope; B*27 and B*57) as well as killer cell immunoglobulin-like receptors have been associated with slow progression of HIV-1 infection. OBJECTIVE: To evaluate the association between serological epitopes HLA-Bw4 and HLA-Bw6 and prognostic markers in AIDS. METHODS: 147 HIV-infected individuals in Bahia, Northeast Brazil, were genotyped for HLA class I locus. HLA class I genotyping was performed by hybridization with sequence-specific oligonucleotide probes following amplification of the corresponding HLA-A, HLA-B and HLA-C genes. Statistical analysis was performed using Fisher's exact and ANOVA tests for categorical and continuous variables, respectively. RESULTS: We detected a significant association (χ2 = 4.856; p = 0.018) between the presence of HLA-Bw4 and low levels of viremia. Eighteen out of the 147 HIV-infected individuals presented viremia <1,800 copies/mL and 129 presented viremia > 2,000 copies/mL. Ninety and four percent (17/18) of all individuals with viremia < 1,800 copies/mL carried HLA-Bw4, compared to 67.4% (87/129) of individuals with viremia > 2,000 copies/mL. Additionally, we found a significantly higher frequency of B*57 (OR = 13.94; 95% CI = 4.19-46.38; p < 0.0001) and Cw*18 (OR = 16.15; 95% CI = 3.46-75.43; p < 0.0001) alleles, favoring the group with lower viremia levels, in comparison with those with higher viral load. CONCLUSION: HLA-Bw4-B*57 and Cw*18 alleles are associated with lower level of viral load in HIV-infected Brazilian patients. These findings may help us in understanding the determinants of HIV evolution in Brazilian patients, as well as in providing important information on immune response correlates of protection for such population.