Molecular analysis of three FUT3 gene single nucleotide polymorphisms and their relationship with the lewis erythrocytary phenotype in a human population of japanese-ancestry living in Tomé Açu, a town in the Brazilian Amazon

The Lewis blood group system involves two major antigens, Le^ª and Le^b. Their antigenic determinants are not primary gene products but are synthesized by the transfer of sugar subunits to a precursory chain by a specific enzyme which is the product of the FUT3 gene (Lewis gene). The presence of three FUT3 gene single nucleotide polymorphisms (SNPs) (59T > G; 508G > A and 1067T > A) was related to the Lewis phenotype of erythrocytes from 185 individuals of Japanese ancestry living in the town of Tomé-Açu in the Brazilian Amazon region. This relationship was detected using a serological hemagglutination test and the Dot-ELISA assay along with the molecular technique PCR-RFLP. We found that the three SNPs investigated in this study only accounted for a proportion of the Lewis-negative phenotype of the erythrocytes.

A constitucionalidade da norma antielisiva no direito tributário brasileiro

Estudo que objetiva demonstrar a constitucionalidade da norma geral antielisiva introduzida no Direito Brasileiro com a alteração do artigo 116, parágrafo único, do Código Tributário Nacional, levando em consideração a atualidade e a interpretação que deve ser realizada em um contexto que necessita observar a Constituição Federal de 1988, com as diversas previsões de direitos sociais. A norma traz à tona a questão do individual frente ao social, da liberdade frente à igualdade, a busca da verdade material sem violação de direitos. Faz-se abordagem do intérprete e a interpretação das normas e princípios, a necessidade da aplicação do princípio da proporcionalidade, com a busca da verdade material e da justiça fiscal sem violação de direitos e garantias fundamentais da pessoa, em especial do contribuinte. Apresentação necessária para desmistificar argumentos positivistas que propositadamente esquecem os fundamentos constitucionais previstos nos artigos 1° e 3° da Constituição Federal de 1988. Paradigmas e preconceitos foram afastados para reconhecer o sentido da norma antielisiva no direito brasileiro. A demonstração da diferença de elisão e evasão fiscal tornou-se indispensável para posteriormente afastar as pedras do caminho, que eram as alegações de inconstitucionalidade da norma antielisão no Brasil, por possível afronta à legalidade e à liberdade. Argumentos foram utilizados com base na Constituição Federal e com o auxílio nas previsões do Novo Código Civil brasileiro. Após a demonstração da constitucionalidade partiu-se para firmar, dentro do contexto, qual o conteúdo do artigo 116, parágrafo único, do CTN, apresentando, ao final, sugestão de procedimentos a serem seguidos para a aplicação da antielisão no Direito brasileiro. Foi realizada pesquisa doutrinária com incursões em leis e na jurisprudência. Propositadamente não foi realizada comparação com legislação estrangeira, fixando como âmbito de estudo, exclusivamente, a aplicação da norma no Brasil.

Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon

The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F_ST coefficients) to the present database ranged from F_ST = 0.0016 between Macapá and Belém to F_ST = 0.0036 between Macapá and the Iberian Peninsula.

Increased risk of venous thrombosis by AB alleles of the ABO blood group and Factor V Leiden in a Brazilian population

Most cases of a predisposition to venous thrombosis are caused by resistance to activated protein C, associated in 95% of cases with the Factor V Leiden allele (FVL or R506Q). Several recent studies report a further increased risk of thrombosis by an association between the AB alleles of the ABO blood group and Factor V Leiden. The present study investigated this association with deep vein thrombosis (DVT) in individuals treated at the Hemocentro de Pernambuco in northeastern Brazil. A case-control comparison showed a significant risk of thrombosis in the presence of Factor V Leiden (OR = 10.1), which was approximately doubled when the AB alleles of the ABO blood group were present as well (OR = 22.3). These results confirm that the increased risk of deep vein thrombosis in the combined presence of AB alleles and Factor V Leiden is also applicable to the Brazilian population suggesting that ABO blood group typing should be routinely added to FVL in studies involving thrombosis.

Molecular analysis of three FUT3 gene single nucleotide polymorphisms and their relationship with the lewis erythrocytary phenotype in a human population of japanese-ancestry living in Tomé Açu, a town in the Brazilian Amazon

The Lewis blood group system involves two major antigens, Le^a and Le^b. Their antigenic determinants are not primary gene products but are synthesized by the transfer of sugar subunits to a precursory chain by a specific enzyme which is the product of the FUT3 gene (Lewis gene). The presence of three FUT3 gene single nucleotide polymorphisms (SNPs) (59T > G; 508G > A and 1067T > A) was related to the Lewis phenotype of erythrocytes from 185 individuals of Japanese ancestry living in the town of Tomé-Açu in the Brazilian Amazon region. This relationship was detected using a serological hemagglutination test and the Dot-ELISA assay along with the molecular technique PCR-RFLP. We found that the three SNPs investigated in this study only accounted for a proportion of the Lewis-negative phenotype of the erythrocytes.