95 resultados para Socio-Spatial Development

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Cet article rassemble des concepts, des théories et des données afin de comprendre comment ils articulent les nouvelles formes de gouvernance du territoire, à travers les stratégies institutionnelles et organisationnelles des acteurs locaux à mobiliser les actifs spécifiques des territoires dans les projets de développement local. Cette étude propose une base théorique pour les études appliquées à essayer de comprendre comment exprimer les organisations et institutions, ses acteurs et ses agents dans l'environnement de la structure de gouvernance territoriale, étant donné les cours divers secteurs socio-spatiale et territoriale au Brésil. Ainsi, les arrangements productifs locaux (APL) et Tours apparaissent comme des stratégies novatrices pour la gouvernance territoriale étant donné le caractère de sa propre configuration de la relation entre l'Etat, privées et autres institutions sociales.

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Pós-graduação em Geografia - IGCE

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Geografia - IGCE

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Since ancient times, the utilization of yeasts by the man has a great impact on the socio-economic development. After the advent of the technology of recombinant DNA, great advances have occurred due to the acquisition of strains of mutant yeasts in the field of applied research, and Saccharomyces cerevisiae has soon been outstanding as an interesting candidate for the expression of heterologous proteins of biotechnological interest. As the time goes by other alternative systems of expression have been shown because they have advantages over Saccharomyces cerevisiae. Among those new systems, Pichia pastoris is outstanding as methylotrophic yeast capable of growing in a culture medium containing methanol as the only source of carbon and energy. The induction of production of glycerol-3-phosphate dehydrogenase (GPD, NAD(+): oxido-redutase EC 1.1. 1.8) by Pichia pastoris was accomplished in the medium containing methanol. One of the most important key parameters in Pichia pastoris expression system is the methanol concentration. Bibliographic reviews on the Pichia pastoris production system have shown that the best culture conditions vary according to the strain used and/or kind of heterologous protein desired to be expressed. Therefore, we have sought to develop a system, involving expression of glycerol-3-phosphate dehydrogenase in the yeast Pichia pastoris, for generating sufficient quantities of the enzyme in order to asses its potential value for use in various food bioanalytical determination. Dehydrogenases have been widely used in the enzymatic assays of diverse composites of industrial interest, being enclosed among them glycerol and a number of important bioanalytical applications.

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Pós-graduação em Educação - IBRC