Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology

A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/ binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LIVIV diagnostic and field epidemiology studies.

Growth of silver nano-particle embedded in tellurite glass: Interaction between localized surface plasmon resonance and Er3+ ions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Surface-plasmon-enhanced frequency upconversion in Pr(3+) doped tellurium-oxide glasses containing silver nanoparticles

A frequency upconversion process in Pr(3+) doped TeO(2)-ZnO glasses containing silver nanoparticles is studied under excitation with a nanosecond laser operating at 590 nm, in resonance with the (3)H(4)-->(1)D(2) transition. The excited Pr(3+) ions exchange energy in the presence of the nanoparticles, originating efficient conversion from orange to blue. The enhancement in the intensity of the luminescence at similar to 482 nm, corresponding to the (3)P(0)-->(3)H(4) transition, is due to the influence of the large local field on the Pr(3+) ions, which are located near the metallic nanoparticles. (C) 2008 American Institute of Physics.

Localized surface plasmon resonance interaction with Er3+-doped tellurite glass

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

SURFACE SOLITARY WAVES IN A DOUBLE DIFFUSIVE SYSTEM

We show that a surface solitary wave governed by the Korteweg-de Vries equation can develop in a fluid acted upon by fluxes of heat and of a second diffusive element. This solitary wave appears as a manifestation of a hydrodynamical instability which sets in only when a certain relation involving the parameters of the system is satisfied.

Tunable plasmon resonance modes on gold nanoparticles in Er 3 +-doped germanium-tellurite glass

Relative to the Er3 +:gold-nanoparticle (Er3 +:Au-NP) axis, the polarization of the gold nanoparticle can be longitudinal (electric dipole parallel to the Er3 +:Au-NP axis) or transverse (electric dipole perpendicular to the Er3 +:Au-NP axis). For longitudinal polarization, the plasmon resonance modes of gold nanoparticles embedded in Er3 +-doped germanium-tellurite glass are activated using laser lines at 808 and 488 nm in resonance with radiative transitions of Er3 + ions. The gold nanoparticles were grown within the host glass by thermal annealing over various lengths of time, achieving diameters lower than 1.6 nm. The resonance wavelengths, determined theoretically and experimentally, are 770 and 800 nm. The absorption wavelength of nanoparticles was determined by using the Frohlich condition. Gold nanoparticles provide tunable emission resulting in a large enhancement for the 2H11/2 → 4I13/2 (emission at 805 nm) and 4S 3/2 → 4I13/2 (emission at 840 nm) electronic transitions of Er3 + ions; this is associated with the quantum yield of the energy transfer process. The excitation pathways, up-conversion and luminescence spectra of Er3 + ions are described through simplified energy level diagrams. We observed that up-conversion is favored by the excited-state absorption due to the presence of the gold nanoparticles coupled with the Er3 + ions within the glass matrix. © 2013 Elsevier B.V.

Large-Area Plasmonic Substrate of Silver-Coated Iron Oxide Nanorod Arrays for Plasmon-Enhanced Spectroscopy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Exploring copper nanostructures as highly uniform and reproducible substrates for plasmon-enhanced fluorescence

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Portable smart films for ultrasensitive detection and chemical analysis using SERS and SERRS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preparação de vidros e vitrocerâmicas de óxidos de metais pesados contendo prata: propriedades ópticas, estruturais e eletroquímicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identification of Rv3852 as a nucleoid-associated protein in Mycobacterium tuberculosis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um sistema de previsão de tempo e de ondas oceânicas para o Atlântico Sul

Um sistema de previsão numérica de tempo e de ondas oceânicas (SPTO) que possa ser operacionalizado no Atlântico Sul é proposto. O SPTO é composto por um modelo atmosférico de área limitada (MAL) e um modelo de ondas de superfície do oceano geradas pelo vento, aplicado em duas versões: uma de malha grossa (MPOMG) e outra de malha fina (MPOMF). O MPOMG abrange uma área de 10(6) km², e tem como finalidade gerar e propagar ondas em regiões remotas à costa brasileira. O MPOMF é aplicado em um domínio 10(4) km² com alta resolução, incorporando irregularidades batimétricas e com as condições iniciais e de fronteiras fomecidas pelo MPOMG. Os modelos utilizam dados de vento à 10 m acima da superfície do oceano. Os arquivos de vento, contendo a evolução espacial e temporais são gerados pelo MAL. Um exemplo de um evento real ocorrido no período de 9 a 11 de agosto de 1988 é apresentado utilizando o acoplamento proposto.

Y2O3:Eu3+ (5 mol%) with Ag nanoparticles prepared by citrate precursor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Plasmonic Coupling in Er3+:Au Tellurite Glass

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nanoscale manipulation of CdSe quantum dots in layer-by-layer films: influence of the host polyelectrolyte on the luminescent properties

Thioglycolic acid-capped Use quantum dots (QDs) were assembled on glass substrates with two distinct polyelectrolytes, viz poly(allylamine hydrochloride) (PAH) and poly(amidoamine) (PAMAM), generation 4 dendrimer, via the layer-by-layer (LbL) technique. Films containing up to 30 polyelectrolyte/QD bilayers were prepared. The growth of the multilayers was monitored with UV-vis spectroscopy, which showed an almost linear increase in the absorbance of the 2.8 nm QDs at 535 nm with the number of deposited bilayers. AFM measurements estimated a film thickness of 3 nm per bilayer for the PAH/Cdse films. The adsorption process and the optical properties of the PAMAM/CdSe LbL films were further analyzed layer-by-layer using surface plasmon resonance (SPR), from which a thickness of 3.2 nm was found for a PAMAM/CdSe bilayer. Photoluminescence measurements revealed higher photooxidation of the quantum dots in PAH/CdSe than in PAMAM/CdSe films. (c) 2004 Elsevier B.V. All rights reserved.