Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Karyotype description of two Neotropical Psittacidae species: the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and the Hawk-headed Parrot, Deroptyus accipitrinus (Psittaciformes: Aves), and its significance for conservation plans

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Chromosomal similarity between the Scaly-headed parrot (Pionus maximiliani), the Short-tailed parrot (Graydidascalus brachyurus) and the Yellow-faced parrot (Salvatoria xanthops) (Psittaciformes: Aves): a cytotaxonomic analysis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

KARYOTYPE STUDIES IN 22 SPECIES OF PARROTS (PSITTACIFORMES, AVES)

The karyotypes of 12 species of Psittaciformes new to cytology are described: Lorius hypoinochrous, L. lory and Phigys solitarius of the Loriidae, and Amazona autumnallis, Aratinga jandaya, Eclectus roratus, Pionus maximiliani, P. menstruus, P. senilis, P. seniloides, Poicephalus senegalus and Polytelis alexandrae of the Psittacidae. The karyotypes of Amazona ochrocephala, Ara ararauna, Ara macao, Psittacula krameri, Psittacus erithacus and Pyrrhura molinae of the Psittacidae have been previously described. For reasons of comparison the karyotypes of Aratinga aurea, Forpus xanthopterygius, Brotogeris sanctithomae and B. versicolorus of the Psittacidae are also described. These karyotypes are compared to those in the literature and the karyological relationships in the Psittaciformes are briefly discussed. Microchromosome fusions and translocations and pericentric inversions probably are responsible for the heterogeneity of karyotypes in the Psittaciformes.

Detecção de Escherichia coli e Salmonella spp. em microbiota intestinal de Psittaciformes em fase de reabilitação para soltura

Escherichia coli is a bacteria of the Enterobacteriacea family and it is part of the enterical microflora of mammals and of many species of birds. Salmonella spp. also belongs to the family Enterobacteriacea, it is responsible for human feed toxinfection outbreaks and usually isolated from domestic and wild birds. The present study analyzed the frequency of both agents in Psittaciformes in rehabilitation process for wildlife reintroduction. In 89 birds analyzed, 19% were infected with E. coli and 1,12% with Salmonella spp. It was carried out an analysis of the profile of antibiotic resistance in which was observed the efficiency of estreptomicin, tetraciclin, trimetoprim and gentamicin over the samples. The samples of E. coli were submitted to the Congo Red Binding test and to the Hemolisis test and 70,6% of positive samples for the first test and 53% for the second one were observed.

Survey on Chlamydophila psittaci in captive ramphastids in São Paulo State, Brazil

Chlamydophila psittaci (C. psittaci) has been detected in 460 avian species, among them the most frequent are the Psittaciformes, Columbiformes, Anseriformes and raptors. In Brazil, the main avian species recognized as healthy carriers belong to the order Psittaciformes and Columbiformes, but very few studies have been done in other bird families. Reports of the occurrence of this disease in the clinical form are rare in the Ramphastids; consequently, they are not commonly evaluated for this agent. The present study reports the investigation of C. psittaci in 25 captive ramphastids from a zoological park in São Paulo State, Brazil. Swabs samples from the cloaca were submitted to semi-nested polymerase chain reaction (semi-nested PCR) for direct detection of the microorganism. Additionally, blood samples obtained from these birds were submitted to the Complement Fixation Test (CFT) for detection of antibodies anti-C. psittaci. The presence of C. psittaci was not detected in the cloacal swab samples tested by the PCR. Nevertheless, 16% (4/25) of the bird's sera were positive by the CFT. Among the species with positive results, there are the saffron toucanet (Pteroglossus bailloni) and black-necked-aracari (Pteroglossus aracari), two species with no descriptions of the survey of C. psittaci published in the literature. Intermittent elimination of C. psittaci is a feature of chronically infected birds; however the absence of a positive-antigen sample did not guarantee that the bird is Chlamydophila-free. The serological results obtained show that the ramphastids tested were previously exposed to the pathogen and developed immune response, but showed no clinical signs of the disease and didn't eliminate regularly the organism in their feces in the moment of the sample collection.

Survey for natural Neospora caninum infection in wild and captive birds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolation of Salmonella enterica Serovar Enteritidis in Blue-Fronted Amazon Parrot (Amazona aestiva)

Avian salmonellosis is a disease caused by bacteria of the genus Salmonella that can cause three distinct diseases in birds: pullorum diseases, fowl typhoid, and paratyphoid infection. Various wildlife species are susceptible to infections by Salmonella, regardless of whether they live in captivity or freely in the wild. The present study verified the presence of Salmonella enterica serovar Enteritidis in three captive specimens of Amazona aestiva. The study involved a total of 103 birds undergoing rehabilitation to prepare for living in the wild, after having been captured from animal traffickers and delivered to the Centrofauna Project of the Floravida Institute in São Paulo, Brazil. This is the first report of Salmonella Enteritidis isolation in A. aestiva that originated from capture associated with animal trafficking; Salmonella was detected during the study by the serologic method of rapid serum agglutination on a plate with bacterial isolate. The antimicrobial profile exam of the isolated samples demonstrated sensitivity to ampicillin, cefaclor, ciprofloxacin, and cloranfenicol. The three samples also presented resistance to more than four antibiotics. The presence of the genes invA and spvC was verified by PCR technique and was associated with virulence and absence of class 1 integron, a gene related to antimicrobial resistance. The commercial antigen for pullorum disease was shown to be a useful tool for rapid detection in the screening of Salmonella of serogroup D(1) in Psittaciformes. New studies on Salmonella carriage in birds involved in trafficking must be performed to better understand their participation in the epidemiologic cycle of salmonellosis in humans and other animals.

Feather mites of Brazil (Acari: Astigmata: Analgoidea and Pterolichoidea)

We present a checklist of feather mites known from native birds in Brazil. The list was compiled from a survey of Brazilian records published in indexing databases (e.g. Zoological Records) and from the available literature. To date, 185 nominal species representing 21 families have been recorded from Brazilian birds. Associations with 15 bird orders were found: Anseriformes, Apodiformes, Caprimulgiformes, Ciconiiformes, Columbiformes, Cuculiformes, Falconiformes, Galliformes, Gruiformes, Opisthocomiformes, Passeriformes, Piciformes, Psittaciformes, Tinamiformes and Trogoniformes. These birds sum to 218 species, which represent only 12.4% of all bird species occurring in Brazil. The feather mite fauna of several species-rich and important bird orders in Brazil remain unexplored, including Cathartiformes, Charadriiformes, Coraciiformes, Galbuliformes and Strigiformes. We estimate that between 900 and 5300 feather mite species are expected to occur on Brazilian birds, which is at least five times greater than current records. The training of researchers with expertise in the taxonomy of this group of mites should be stimulated so that there is a compatible number of taxonomists to discover and describe the almost unexplored feather mite fauna in Brazil.

Interspecies variation in orbital bone structure of psittaciform birds (with emphasis on Psittacidae)

Objectives: This text presents an anatomical study of the normal bony orbital structure of a sample of different bird species belonging to the order Psittaciformes.Procedures: the bony anatomy of Psittaciformes' skulls was examined and described using cadavers of birds that were presented already dead to the Federal University of Parana, Brazil or had been euthanized for humane reasons. Dissections of the orbital cavity were performed under 2-4 x magnification, and descriptions of the orbital bones were made from observations of macerated skulls that had been boiled and cleaned. The present paper discusses the main features of the bony orbit of psittaciform birds, describing known anatomical information but also bringing new information, mainly concerning species differences that might help not only veterinary anatomists but also zoologists, clinicians, researchers, and students of veterinary ophthalmology to better comprehend this order of birds.Results and conclusions: Variations in the anatomic conformation of the bony elements of the orbit were observed in different species of Psittaciformes. Based on these differences, Psittaciformes were classified into two different groups. The first group of Psittaciformes shows an enclosed (complete) bony orbit formed by the junction of the orbital with the postorbital processes, creating a suborbital arch. The second group of Psittaciformes essentially lacked a suborbital arch, presenting an open (incomplete) bony orbit, typical of most modern birds. In the latter group, orbital and postorbital processes are present.

Cryptococcosis outbreak in psittacine birds in Brazil

An outbreak of cryptococcosis occurred in a breeding aviary in São Paulo, Brazil. Seven psittacine birds (of species Charmosyna papou, Lorius lory, Trichoglossus goldiei, Psittacula krameri and Psittacus erithacus) died of disseminated cryptococcosis. Incoordination, progressive paralysis and difficulty in flying were seen in five birds, whereas superficial lesions coincident with respiratory alterations were seen in two birds. Encapsulated yeasts suggestive of Cryptococcus sp. were seen in faecal smears stained with India ink in two cases. Histological examination of the birds showed cryptococcal cells in various tissues, including the beak, choana, sinus, lungs, air sacs, heart, liver, spleen, kidneys, intestines and central nervous system. High titres of cryptococcal antigen were observed in the serum of an affected bird. In this case, titres increased during treatment and the bird eventually died. Yeasts were isolated from the nasal mass, faeces and liver of one bird. Cryptococcus neoformans var. gattii serovar B was identified based on biochemical, physiological and serological tests. These strains were resistant (minimum inhibitory concentration 64 μ g/ml) to fluconazole. This is the first report of C. neoformans var. gattii occurring in psittacine birds in Brazil. © 2004 ISHAM.