Poultry offal meal in chicken: Traceability using the technique of carbon (C-13/C-12)- and nitrogen (N-15/N-14)-stable isotopes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds

The objective of the present study was to trace the inclusion of poultry offal meal (POM) in the diet of meat-type quails reared for a long period using the technique of stable isotopes. A number of 320 quails were randomly distributed into eight treatments: vegetable diet (T1), and a diet containing 8% POM were fed until the end of the experimental period (T2) or replaced by the vegetable diet on day 42 (T3), 56 (T4), 70 (T5), 84 (T6), 98 (T7), and 112 (T8). Breast muscle samples were collected from four birds randomly selected per treatment every 14 days. The obtained isotope results were submitted to multivariate analysis of variance (MANOVA) with the aid of the GLM procedure of statistical SAS program. Treatments were different from T1 when birds were sacrificed at least two weeks after the diet was changed. T2 results were different from T1 in all evaluated periods. It was concluded that it is possible to trace poultry offal meal inclusion in a strictly vegetable diet after the diet was changed for at least 14 days.

Poultry offal meal in broiler chicken feed

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Traceability of poultry offal meal in broiler feeding using isotopic analysis (delta C-13 and delta N-15) of different tissues

Our goal was to trace the inclusion of poultry offal meal (OM) in diets by using carbon (13C/12C) and nitrogen (15N/14N) isotopic ratios of different tissues in order to contribute for the development of an independent technology for the certification of the feeding of broilers reared on diets with no addition of animal ingredients. Eighty one-day-old chicks were randomly distributed into five experimental treatments, that is, diets containing increasing levels of OM inclusion (0, 2, 4, 8 and 16% OM), with four replicates of four birds each. At 42 days of age, four birds per treatment (n=4) were randomly selected, weighed, and sacrificed to collect breast muscle (Pectoralis major), keel and tibia samples to determine their isotopic ratios (13C/12C e 15N/14N). It was observed that 13C and 15N enrichment increased as a function of increasing OM inclusion in all diets. The analyses of the Pectoralis major showed that that only treatments with 8 and 16% OM dietary inclusion were different form those in the control group (0% OM). on the other hand, when the keel and tibia were analyzed, in addition to 8 and 16% OM), the treatment with 4% OM inclusion was also different from the control group. The use of isotopic ratios of stable carbon and nitrogen isotopes is an alternative to trace OM inclusion in broiler diets as it is capable of tracing OM levels below those usually practiced by the poultry industry in Brazil.

Poultry offal meal traceability in meat quail tissues using the technique of stable carbon (13C/12C) and nitrogen (15N/14N) isotopes

Studies on the detection of animal by-products in poultry meat are rare, and non-existent on quail meat. This study aimed at detectiong increasing levels of poultry offal meal (POM) in quail meat, using carbon (13C/12C) and nitrogen (15N/14N) stable isotopes technique. Sixty four on-day-old male quails derived from a commercial farm were randomly distributed into seven different groups, which were fed experimental diets containing 0, 1.5, 3.0, 4.5, 6.0, 7.5, and 15% of POM. Diets were formulated to contain equal energy, protein, and amino acid levels. Four individuals per treatment were sacrificed at 42 days of age for breast muscle (Pectoralis major), keel, and tibia collection, which were subsequently submitted to analyses. Isotopic δ13C and δ15N enrichment was observed in all analyzed tissues, with the lowest detection level of 3% dietary inclusion of poultry offal meal.

Effect of poultry viscera meal inclusion in broiler diets in different rearing periods on performance, carcass and parts yields

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Chemical composition and apparent digestibility of the ostrich offal meal for Nile tilapia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Rastreabilidade de subprodutos de origem animal em dietas com levedura e trigo para frangos

O objetivo deste trabalho foi rastrear a inclussão de farinhas de origem animal em rações para frango de corte com ou sem levedura de cana-de-açúcar e farelo de trigo, por meio da análise do músculo peitoral das aves pelas técnicas dos isótopos estáveis de carbono e nitrogênio. Foram utilizados 210 pintos machos (Cobb), com um dia de idade, distribuídos aleatoriamente em sete tratamentos de 30 aves cada, tendo sido um tratamento controle (dieta vegetal) e seis com inclusão de farinha de carne e ossos bovina ou farinha de vísceras de aves na dieta, com ou sem levedura de cana-de-açúcar e farelo de trigo. Aos 42 dias de idade, foram abatidas quatro aves, por tratamento, escolhidas ao acaso, cujo músculo peitoral foi retirado para análise da razão isotópica. Os resultados obtidos foram submetidos à análise multivariada. Os tratamentos experimentais diferiram do tratamento controle, e foi identificada a inclusão de farinha de origem animal, pelas técnicas dos isótopos estáveis, mesmo com inclusão de levedura ou farelo de trigo na dieta.

Traceability of animal byproducts in quail (Coturnix coturnix japonica) tissues using carbon (13C/12C) and nitrogen (15N/14N) stable isotopes

Consistent information on meat products consumed by the public is essential. The technique of stable isotopes is a powerful tool to recover consumers' confidence, as it allows the detection of animal byproduct residues in poultry meat, particularly in quail meat. This study aimed at checking the presence of poultry byproduct mixtures in quail diets by applying the technique of carbon (13C/12C) and nitrogen (15N/14N) stable isotopes in quail breast muscle, keel, and tibia. Sixty four one-day-old male quails were obtained from a commercial farm. Birds were housed in an experimental house from one to 42 days of age, and were randomly distributed into 8 experimental treatments, and fed diets containing poultry offal meal (POM), bovine meat and bone meal (MBM) or poultry feather meal (PFM), or their mixtures. Four birds per treatment were slaughtered at 42 days of age, and breast (Pectoralis major), keel, and tibia were collected for analyses. The inclusion of animal byproducts in quail diets was detected by 13C e 15N analyses in the tissues of the birds; however, it was not possible to specify which byproducts were used. It was concluded that quail meat can be certified by the technique of stable isotopes.

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Traceability of animal by product meals in broilers fed sugarcane yeast using stable isotopes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Salmonella spp. e Clostridium perfringens em farinhas de origem animal utilizadas na fabricação de rações e avaliação de aditivo na inibição de patógeno

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção de farinha de vísceras nas fases de alimentação em frangos de corte pela técnica de isótopos estáveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sunflower meal in commercial layer diets formulated on total and digestible amino acids basis

An experiment was conduced to evaluate the inclusion of sunflower meal (SBM) in commercial layer diets formulated on total or digestible amino acids basis. One hundred forty-four 41-week-old Lohmann LSL layers were distributed in a completely randomized experimental design in a 2 x 4 factorial arrangement with three replications of six birds each. Treatments consisted of a combination of four SBM inclusion levels SBM(0%, 4%, 8%, and 12%) and feed formulation according two amino acid recommendations (total or digestible). The experimental period was divided into five periods of fourteen days. Performance parameters (egg production, feed intake, feed conversion, egg mass) were evaluated for each period. In the last two days of each period, three eggs per replication were collected to evaluate egg quality (Haugh units, specific gravity, egg weight, eggshell thickness, and eggshell percentage). Hens fed on total amino acid recommendation presented the highest values for egg weight. Diets formulated on digestible amino acids basis showed an improvement in eggshell percentage and egg specific gravity. SBM addition in commercial layer diets did not influence performance; however, increasing SBM dietary levels SBM improved eggshell quality.

Utilização de subprodutos de origem animal em dietas para frangos de corte com base no conceito de proteínas bruta e ideal, no período de 43 a 49 dias de idade

Foi conduzido um experimento para avaliar a utilização de subprodutos de origem animal em rações para frangos de corte, formuladas com base nas proteínas bruta e ideal no período de 43 a 49 dias de idade. Foram utilizados 600 frangos machos distribuídos em arranjo fatorial 2x2+1, com duas fontes de proteína de origem animal (farinha de vísceras de aves e farinha de sangue bovino), dois conceitos de formulação (proteínas bruta e ideal) e uma ração testemunha à base de milho e de farelo de soja, com quatro repetições cada. As características avaliadas foram ganho de peso, consumo de ração, conversão alimentar, rendimento de carcaça, peito e porcentagem de gordura abdominal. Os melhores ganho em peso e conversão alimentar foram obtidos quando a dieta à base de milho e farelo de soja foi utilizada. As farinhas de vísceras e de sangue e os conceitos de formulação não exerceram influência sobre as características avaliadas.