Isolation and growth of the symbiotic fungus of Atta capiguara (Hymenoptera: Formicidae)

This work's objectives were to isolate and evaluate the growth of the symbiotic fungus of Atta capiguara Gonçalves on artificial medium, under different pH and temperature conditions. Isolation was accomplished using the following media: Sabouraud, oat-agar, PDA, and PDA with the addition of extracts from the grasses Paspalum sp. Flügge and Hyparrhenia rufa (Nees) Stapf.. The medium used in the growth study was PDA with the addition of a Paspalum sp. (0.22%, w/v) extract at initial pH values of 4.5, 6.0, and 7.5. Mycelium disks were transferred to plates containing the culture medium. The plates were maintained at temperatures of 20, 23, and 26 ± 1°C. Mycelial radial growth evaluations were performed at 7, 14, 21, 28, and 35 days of incubation. Fungus isolation was obtained in all media studied. The highest radial means were obtained at initial pH values of 6.0 and 7.5 and temperatures of 23 and 26± 1°C. Greater plot losses occurred at the initial pH condition of 7.5. In general, A. capiguara fungi can be grown in the medium studied, at an initial pH of 6.0 and temperatures of 23 or 26± 1°C. Radial growth evaluations at 14 and 28 days of incubation can be recommended for substrate studies involving the symbiotic fungus.

Desenvolvimento e composição mineral do picão-preto sob diferentes níveis de pH

Visando estudar a tolerância do picão-preto (Bidens pilosa) a diferentes níveis de pH do substrato, conduziu-se um ensaio em condições de câmara de crescimento e utilizando-se a técnica de hidroponia, no qual três plântulas foram transplantadas para recipientes contendo solução nutritiva de Hoagland & Arnon, com pH ajustado para 3,5, 4,0, 5,0, 6,0, 7,0 e 8,0. O delineamento experimental utilizado foi o inteiramente casualizado com três repetições. Ao final do período experimental, 42 dias após o transplante, observou-se que as plantas que se desenvolveram nos pHs 3,5 e 8,0 apresentaram-se menores, com menor comprimento e volume do sistema radicular, menor número de folhas vivas e, consequentemente, menor peso de matéria seca das folhas, caule e raízes. Os teores de N e S nas raízes diminuiram com o aumento do pH do substrato, enquanto os de Ca e Mg aumentaram e os de K e P diminuiram no pH 5,0 a 6,0. No caule, apenas o teor de Mg foi afetado pelo pH, mostrando resposta positiva. Na folha obteve-se os maiores teores de P, S, Ca e Mg quando a planta se desenvolveu em pH 6,0. Não se constatou diferença significativa entre os efeitos dos pHs 5,0, 6,0 e 7,0 nos parâmetros avaliados, sendo que foi no pH 6,0 que se estimou, através de análise de regressão, melhor desenvolvimento do picão-preto. Concluiu-se que o picãopreto apresenta melhor desenvolvimento em pHs próximo ao neutro, com certa tolerância a pHs extremos (3,5 e 8,0).

Screening of culture condition for xylanase production by filamentous fungi

The objective of this research was to investigate xylanase production by filamentous fungi (Trichoderma viride) to determine the best cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of this fungus was separately evaluated in two solid substrates (wheat and soybean bran) and in Vogel medium, pure and by adding other carbon sources. Temperature effects, cultivation time, and spore concentrations were also tested. The best temperature and carbon source for enzyme and biomass production was 25 C and sorbitol, respectively. Maximum xylanase activity was achieved when the fungus was cultivated in wheat bran along with sorbitol (1%, w/v), using a spore concentration of 2 x 10(6) spores. mL(-1), pH 5.0, for 144 h cultivation. The study demonstrated not only the importance of the nature of the substrate in obtaining a system resistant to catabolic repression, but also the importance of the culture conditions for biosynthesis of this enzyme. T. viride showed a high potential for xylanase production under the conditions presented in these assays.

Resin Bonding to a Feldspar Ceramic After Different Ceramic Surface Conditioning Methods: Evaluation of Contact Angle, Surface pH, and Microtensile Bond Strength Durability

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Adesão de um cimento resinoso a uma cerâmica feldspática: avaliação da resistência adesiva, ângulo de contato e ph, em função de diferentes técnicas de condicionamento

Pós-graduação em Odontologia Restauradora - ICT

Fadiga axial do aço inoxidável 15-5 PH tratado pela implantação iônica por imersão em plasma

When materials for application in aircraft structural components are studied, it must be considered that they will be submitted to cyclic loading, and this is an important parameter to design the study in fatigue life of the materials. Whereas, for example, a landing gear operation, the study of fatigue life and corrosion in the materials used in it is essential, especially when you want to use new techniques for surface treatments. The objective is to study the influence of surface treatment of immersion ion implantation nitrogen plasma, in axial fatigue of Stainless steel 15-5 PH in 39-42 HRC condition. Stainless steel 15-5 PH was tested in axial fatigue and corrosion in salt spray. It was also performed microindentation tests, optical microscopy for microstructural analysis and scanning electron microscopy for fractographic analysis. It was observed that the 3IP had no effect on the thickness of the material and not the hardness of it, and still provided a significant increase in fatigue life of the material

Avaliação do pH na amostra isolada e na urina de 24h de pacientes litiásicos em uso de citrato de potássio

Pós-graduação em Bases Gerais da Cirurgia - FMB