Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Peregrinação, conquista e povoamento. Mito e realismo desencantado numa hagiografia medieval portuguesa

O texto pretende realizar uma interpretação da narrativa de viagem ao Paraíso Terrestre cistercience Vida de Santo Amaro, à luz da história portuguesa entre os séculos XIII e XV, apontando as relações existentes entre pensamento histórico e pensamento mítico na passagem do processo de Reconquista para os Descobrimentos, vivenciados ao longo da história das diferentes versões escritas da fonte analisada.

Geografia: o olhar e a imagem pictórica

Este artigo objetiva apresentar, de forma introdutória, a possibilidade e a necessidade de diálogo entre a linguagem pictórica e o discurso científico da Geografia. Para tal, apresenta alguns elementos do alfabeto das pinturas de imagens a partir tanto da semiótica quanto da gestalt, analisadas por Fayga Ostrower e Donis Dondis. O exercício interpretativo dá-se a partir do conceito de paisagem aplicado sobre as imagens elaboradas artisticamente por grandes pintores da humanidade, contextualizando-as a partir dos elementos técnicos e tecnológicos que existiam na época e no lugar, e da forma como esses interferem nos processos de elaboração e interpretação das obras. Para exemplificar isso, utilizam-se três pinturas elaboradas em diferentes períodos, a partir da Europa medieval até o mundo moderno, apresentando, assim, os aspectos gerais que levavam à mudança de leitura espacial do território produzido a partir da consolidação do mundo urbano, industrial e baseado na lógica da mercadoria.

A história portuguesa medieval: preservação, ordenação e esquecimento

Os últimos séculos da Idade Média, nomeadamente os séculos XIII, XIV e XV, são marcados na Europa por uma crescente preocupação em fixar por escrito os diversos saberes e eventos, preocupação que, ligada às tentativas de sistematizar a organização dos reinos, contribui significativamente para a ascensão das línguas vulgares como línguas dos mais variados gêneros - de documentos jurídicos e administrativos a textos de caráter filosófico e histórico. em Portugal, o empenho em deixar registrado o passado numa língua acessível tem início no século XIV e culmina no século XV, quando se procura organizar a memória através da escrita e se começa a construir uma perspectiva portuguesa sobre o passado. O objetivo do presente texto é mapear alguns índices que revelam a importância que os portugueses dos séculos XIV e XV conferem à ordenação do passado, sobretudo a partir da escolha do que devia e do que não podia cair no esquecimento.

Chromosomal evolution in Gekkonidae. I. Chromosome painting between Gekko and Hemidactylus species reveals phylogenetic relationships within the group

Geckos are a large group of lizards characterized by a rich variety of species, different modes of sex determination and diverse karyotypes. In spite of many unresolved questions on lizards' phylogeny and taxonomy, the karyotypes of most geckos have been studied by conventional cytogenetic methods only. We used flow-sorted chromosome-specific painting probes of Japanese gecko (Gekko japonicus), Mediterranean house gecko (Hemidactylus turcicus) and flat-tailed house gecko (Hemidactylus platyurus) to reveal homologous regions and to study karyotype evolution in seven gecko species (Gekko gecko, G. japonicus, G. ulikovskii, G. vittatus, Hemidactylus frenatus, H. platyurus and H. turcicus). Generally, the karyotypes of geckos were found to be conserved, but we revealed some characteristic rearrangements including both fissions and fusions in Hemidactylus. The karyotype of H. platyurus contained a heteromorphic pair in all female individuals, where one of the homologues had a terminal DAPI-negative and C-positive heterochromatic block that might indicate a putative sex chromosome. Among two male individuals studied, only one carried such a polymorphism, and the second one had none, suggesting a possible ZZ/ZW sex determination in some populations of this species. We found that all Gekko species have retained the putative ancestral karyotype, whilst the fission of the largest ancestral chromosome occurred in the ancestor of modern Hemidactylus species. Three common fissions occurred in the ancestor of Mediterranean house and flat-tailed house geckos, suggesting their sister group relationships. PCR-assisted mapping on flow-sorted chromosome libraries with conserved DMRT1 gene primers in G. japonicus indicates the localization of DMRT1 gene on chromosome 6.

Color alteration of the paint used for iris painting in ocular prostheses

The purpose of this study was to assess color alteration of the paints used for iris painting in artificial eyes. Five disks of heat cured acrylic resin were confectioned by microwave energy for each paint analyzed, in a total of 40 specimens. Each specimen consisted of a colorless acrylic resin disk and another of equal size, of scleral white colored acrylic resin, with the painting interposed between the two disks. The specimens were submitted to an accelerated aging process in a chamber under ultraviolet radiation for 1,008 hours. To assess color variation, a reflective spectrophotometer was used. The results were statistically analyzed by ANOVA and the Tukey test (p < 0.05). All the paints underwent chromatic alteration. The oil paint presented the highest resistance to accelerated aging.

Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting

The stingless bee Partamona helleri in southeast Brazil shows the regular chromosome number 2n = 34 and a variable number of up to four minute B1 or B2 chromosomes. Previous cytogenetic analyses have indicated morphological similarities between the B1 chromosome and chromosome segments in the regular karyotype. In this study, microdissection and chromosome painting were employed along with C banding, NOR banding, and base-specific fluorochrome staining to investigate the origin of the B1 chromosome in P. helleri. B1-generated probe hybridized exclusively to B1 chromosomes. This result suggests an independent origin from the regular karyotype or, alternatively, that the B chromosome may have suffered substantial genetic alterations along its independent evolution. The absence of higher dosages of these small B chromosomes in this population of P. helleri may be related to the existence of either a genetic or cytogenetic constraint in the establishment of such high numbered karyotypes. © 2012 INRA, DIB and Springer-Verlag, France.

Chromosome painting of Z and W sex chromosomes in Characidium (Characiformes, Crenuchidae)

Some species of the genus Characidium have heteromorphic ZZ/ZW sex chromosomes with a totally heterochromatic W chromosome. Methods for chromosome microdissection associated with chromosome painting have become important tools for cytogenetic studies in Neotropical fish. In Characidium cf. fasciatum, the Z chromosome contains a pericentromeric heterochromatin block, whereas the W chromosome is completely heterochromatic. Therefore, a probe was produced from the W chromosome through microdissection and degenerate oligonucleotide-primed polymerase chain reaction amplification. FISH was performed using the W probe on the chromosomes of specimens of this species. This revealed expressive marks in the pericentromeric region of the Z chromosome as well as a completely painted W chromosome. When applying the same probe on chromosome preparations of C. cf. gomesi and Characidium sp., a pattern similar to C. cf. fasciatum was found, while C. cf. zebra, C. cf. lagosantense and Crenuchus spilurus species showed no hybridization signals. Structural changes in the chromosomes of an ancestral sexual system in the group that includes the species C. cf. gomesi, C. cf. fasciatum and Characidium sp., could have contributed to the process of speciation and could represent a causal mechanism of chromosomal diversification in this group. The heterochromatinization process possibly began in homomorphic and homologous chromosomes of an ancestral form, and this process could have given rise to the current patterns found in the species with sex chromosome heteromorphism. © 2013 Springer Science+Business Media Dordrecht.

Comparative analysis of sex chromosomes in Leporinus species (Teleostei, Characiformes) using chromosome painting

Background: The Leporinus genus, belonging to the Anostomidae family, is an interesting model for studies of sex chromosome evolution in fish, particularly because of the presence of heteromorphic sex chromosomes only in some species of the genus. In this study we used W chromosome-derived probes in a series of cross species chromosome painting experiments to try to understand events of sex chromosome evolution in this family.Results: W chromosome painting probes from Leporinus elongatus, L. macrocephalus and L. obtusidens were hybridized to each others chromosomes. The results showed signals along their W chromosomes and the use of L. elongatus W probe against L. macrocephalus and L. obtusidens also showed signals over the Z chromosome. No signals were observed when the later aforementioned probe was used in hybridization procedures against other four Anostomidae species without sex chromosomes.Conclusions: Our results demonstrate a common origin of sex chromosomes in L. elongatus, L. macrocephalus and L. obtusidens but suggest that the L. elongatus chromosome system is at a different evolutionary stage. The absence of signals in the species without differentiated sex chromosomes does not exclude the possibility of cryptic sex chromosomes, but they must contain other Leporinus W sequences than those described here. © 2013 Parise-Maltempi et al.; licensee BioMed Central Ltd.

Da geografia medieval às origens da geografia moderna: contrastes entre diferentes noções de natureza, espaço e tempo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

De versos e trovas: análise de aspectos fonoestilísticos do português medieval por meio das “Cantigas de Santa Maria”

Pós-graduação em Linguística e Língua Portuguesa - FCLAR

Rumores da escrita, vestígios do passado: uma interpretação fonológica das vogais do português arcaico por meio da poesia medieval

A proposta do presente livro é oferecer ao leitor uma descrição fonológica das qualidades vocálicas vigentes na primeira fase (período trovadoresco) do português arcaico a partir da análise das rimas e da grafia das Cantigas de Santa Maria, de Afonso X, o Sábio, Rei de Leão e Castela, elaboradas na segunda metade do século XIII.