Reduction capability of soil humic substances from the Rio Negro basin, Brazil, towards Hg(II) studied by a multimethod approach and principal component analysis (PCA)

This paper characterizes humic substances (HS) extracted from soil samples collected in the Rio Negro basin in the state of Amazonas, Brazil, particularly investigating their reduction capabilities towards Hg(II) in order to elucidate potential mercury cycling/volatilization in this environment. For this reason, a multimethod approach was used, consisting of both instrumental methods (elemental analysis, EPR, solid-state NMR, FIA combined with cold-vapor AAS of Hg(0)) and statistical methods such as principal component analysis (PCA) and a central composite factorial planning method. The HS under study were divided into groups, complexing and reducing ones, owing to different distribution of their functionalities. The main functionalities (cor)related with reduction of Hg(II) were phenolic, carboxylic and amide groups, while the groups related with complexation of Hg(II) were ethers, hydroxyls, aldehydes and ketones. The HS extracted from floodable regions of the Rio Negro basin presented a greater capacity to retain (to complex, to adsorb physically and/or chemically) Hg(II), while nonfloodable regions showed a greater capacity to reduce Hg(II), indicating that HS extracted from different types of regions contribute in different ways to the biogeochemical mercury cycle in the basin of the mid-Rio Negro, AM, Brazil. (c) 2007 Published by Elsevier B.V.

Fotossíntese, reservas orgânicas e rebrota do capim-mombaça (Panicum maximum Jacq.) sob diferentes intensidades de desfolha do perfilho principal

Buscando-se avaliar morfofisiologicamente a rebrota do capim-mombaça, quatro desfolhas foram impostas ao perfilho principal, sendo estudado o comportamento da planta em termos da taxa de expansão da área foliar, crescimento do sistema radicular, nível de carboidratos totais não estruturais (CTNE) da raiz e do colmo, taxa de crescimento relativo (TCR), taxa de assimilação líquida (TAL) e razão de área foliar (RAF) às idades de 2, 5, 9 e 16 dias após as desfolhas, bem como da taxa fotossintética máxima às idades de 2, 6 e 13 dias das folhas remanescentes à desfolha. As desfolhas foram as seguintes: remoção de todas as lâminas foliares (desfolha total), a remoção da lâmina da folha adulta mais jovem (desfolha superior), a remoção das lâminas das duas folhas adultas mais velhas (desfolha inferior) e controle (sem desfolha), juntamente com o corte dos demais perfilhos, realizado a 8 cm do solo. Foram observadas cinco repetições por tratamento, segundo o delineamento inteiramente casualizado. As folhas adultas não diferiram quanto às taxas fotossintéticas máximas, que exibiram aumento nos primeiros dias após a desfolha, e queda aos 13 dias. A desfolha reduziu os teores de CTNE da base do colmo, principalmente nas plantas sob desfolha total. Comprometimento do crescimento do sistema radicular e do teor de CTNE das raízes foi observado nas plantas sob desfolha total, que também tiveram sua TCR reduzida nos primeiros dias de rebrotação. Entretanto, o aumento na RAF possibilitou a estas plantas recuperação da TCR e alta taxa de expansão da área foliar, igualando a área foliar das demais plantas aos 16 dias de rebrota.

Short communication: Principal components and factor analytic models for test-day milk yield in Brazilian Holstein cattle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Participação do colmo principal e dos afilhos na produtividade do arroz irrigado, em função da densidade de semeadura

Entre as práticas culturais a serem consideradas na implantação da cultura do arroz destaca-se a densidade de semeadura, que deve estabelecer, em grande parte, a participação do colmo principal e dos afilhos nos componentes da produção, possibilitando a obtenção da máxima produtividade. O trabalho teve como objetivo avaliar a participação do colmo principal e dos afilhos na produtividade de grãos de arroz, cv. IAC 102, no sistema irrigado por inundação, em função da densidade de semeadura. O experimento foi desenvolvido sob túnel plástico, em Botucatu (SP), em caixas d'água de cimento amianto de 500 L, contendo Neossolo Flúvico Ta Eutrófico, com profundidade de 30 cm. O delineamento experimental foi o inteiramente casualizado, com quatro repetições. As densidades de semeadura foram: 100, 200, 300, 400, 500 e 600 sementes viáveis por m², em 4 linhas de 1 m por caixa, espaçadas com 20 cm. A elevação da densidade de semeadura diminui o afilhamento e proporciona a maior participação dos colmos principais, porém, não resultando em incremento de produtividade, devido à plasticidade das plantas de arroz, que proporciona o ajustamento dos componentes da produção.

Caracterização e hidrólise in vitro da globulina principal de grão-de-bico (Cicer arietinum L.), var. IAC-Marrocos

No presente estudo procedeu-se ao isolamento e caracterização da fração globulina majoritária (11 S) de grão-de-bico, var. IAC-Marrocos. A globulina majoritária extraída foi isolada por cromatografia de filtração em gel e de troca-iônica mostrando apenas uma banda de proteína na eletroforese em gel de poliacrilamida. A globulina majoritária, após passagem em coluna de Sephadex, revelou duas bandas protéicas de 55 e 52,5kDa e três bandas menores em gel de poliacrilamida dodecilsulfato de sódio. Na presença de 2-mercaptoetanol 6 polipeptídios na faixa de 18 a 42kDa foram revelados na eletroforese. A globulina isolada foi submetida à ação da tripsina e quimotripsina onde a forma nativa mostrou-se resistente à ação enzimática enquanto o aquecimento (96 e 121°C/15min) não foi suficiente para aumentar a susceptibilidade à hidrólise, significativamente. Adição de NaCl 0,3M levou a um aumento da estabilidade estrutural com menor susceptibilidade à digestão proteolítica, fato em parte perdido com o aquecimento. As hidrólises foram acompanhadas por eletroforese em gel de poliacrilamida dodecilsulfato de sódio.

Morphological Features of the Apical Region in the Principal Cells of Mongrel Dog Epididymis

The epithelial principal cells are the predominant cell type of the epididymis. These cells have been shown to be both secretory and endocytic cells. The apical region of the cytoplasm of principal cells in the mongrel dog are located close to the cell apex and tubular lumen, and shown microvilli at the luminal border and present a endocytic apparatus, that consists of coated pits and vesicles, endosomes of varying size, multivesicular bodies, and lysosomes. The endosomes, multivesicular bodies and lysosomes contained the electron-dense patches. These results suggest that principal cells of the epididymis in the dog as possess a highly developed endocytic apparatus play a role in endocytosis. These cells function are similarly to the related in other mammals, in performing endocytosis.

Mapping quantitative trait loci in Gallus gallus using principal components

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Representation and analysis of soccer players' actions using principal components

This paper proposes the use of the Principal Components Analysis (PCA) method to represent and to analyse soccer players' actions distribution in the pitch. The seven games of the Brazilian National Team during the 2002 World Cup were analysed. The player's position actions were measured from videotapes in a computer interface. The results were: a) the graphical representation, given by two orthogonal segments in the two directions of maximal variability and centred at the mean of each player's actions position; b) the eccentricity measurement, given by the variability ratio and c) the actions zone area, given by variability product. The results showed that the individual characteristics of acting were well represented by the PCA, allowing comparisons among games and providing insights related to the tactical organisation of the team.

Chemosystematic studies of natural compounds isolated from Asteraceae: characterization of tribes by principal component analysis

This work describes an application of principal component analysis (PCA) on a database of secondary metabolites from the Asteraceae family. The numbers of occurrences of metabolites in 11 chemical classes for the different vibes of the family were used as variables, PCA allows the identification of chemical classes that contribute most to the subgroups classification within the family. Relationships between chemical composition and botanical classification were made. (C) 2001 Elsevier B.V. B.V. All rights reserved.

A partial least squares and principal component regression study of quinone compounds with trypanocidal activity

A quantitative structure-activity relationship (QSAR) study of 19 quinone compounds with trypanocidal activity was performed by Partial Least Squares (PLS) and Principal Component Regression (PCR) methods with the use of leave-one-out crossvalidation procedure to build the regression models. The trypanocidal activity of the compounds is related to their first cathodic potential (Ep(c1)). The regression PLS and PCR models built in this study were also used to predict the Ep(c1) of six new quinone compounds. The PLS model was built with three principal components that described 96.50% of the total variance and present Q(2) = 0.83 and R-2 = 0.90. The results obtained with the PCR model were similar to those obtained with the PLS model. The PCR model was also built with three principal components that described 96.67% of the total variance with Q(2) = 0.83 and R-2 = 0.90. The most important descriptors for our PLS and PCR models were HOMO-1 (energy of the molecular orbital below HOMO), Q4 (atomic charge at position 4), MAXDN (maximal electrotopological negative difference), and HYF (hydrophilicity index).

Alcohol effects on the principal and clear cells of the caput epididymis of albino rats

Ultrastructural observations of principal cells of the epithelium lining of the proximal caput epididymis in experimental alcoholic albino rats at 180 days of treatment showed pyknotic nuclei, ill-defined cellular organelles and clusters of electrondense bodies, perhaps lysosomes. It was also verified for a progressive accumulation of lipid droplets initially in the basal and perinuclear cytoplasm and finally in the apical cytoplasm of principal cells at 60, 120 and 180 days of experimentation, respectively. The clear cells of alcoholic rats at 180 days showed the cytoplasm totally filled with lipid droplets. These findings were taken comparatively with the morphological features of the same epididymal cells in control (normal) rats.

Estimates of genetic parameters, and cluster and principal components analyses of breeding values related to egg production traits in a White Leghorn population

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The use of principal components and univariate charts to control multivariate processes

In this article, we evaluate the performance of the T2 chart based on the principal components (PC chart) and the simultaneous univariate control charts based on the original variables (SU X̄ charts) or based on the principal components (SUPC charts). The main reason to consider the PC chart lies on the dimensionality reduction. However, depending on the disturbance and on the way the original variables are related, the chart is very slow in signaling, except when all variables are negatively correlated and the principal component is wisely selected. Comparing the SU X̄, the SUPC and the T 2 charts we conclude that the SU X̄ charts (SUPC charts) have a better overall performance when the variables are positively (negatively) correlated. We also develop the expression to obtain the power of two S 2 charts designed for monitoring the covariance matrix. These joint S2 charts are, in the majority of the cases, more efficient than the generalized variance |S| chart.