Protein-Ion Binding Process on Finite Macromolecular Concentration. A Poisson-Boltzmann and Monte Carlo Study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of Antibacterial Agents on Dental Pulps of Monkeys Mechanically Exposed and Contaminated.

The purpose of this study was to compare the effectiveness of antibacterial agents and mineral trioxide aggregate in the healing of bacterial contaminated primate pulps. Study Design: The experiment required four adult male primates (Cebus opella) with 48 teeth prepared with buccal penetrartions into the pulpal tissues. The preparations (Cebus opella) with 48 teeth prepared with buccal penetrations into the exposed to cotton pellets soaked in a bacterial mixture consisting of microorganisms normally found in human pulpal abscesses obtained from the Endodontic Clinic of UNESP. Following bacterial inoculation (30 minute exposure), the pulpal tissue was immediately treated with either sterile saline, Cipro HC Otic solution (12), diluted Buckley formecresol solution (12) or Otosporin otic solution (12) for 5 minutes. After removal of the pellet, hemostasis was obtained and a ZOE base applied to the DFC treated pulps and the non-treated controls (12). After hemostasis, the other exposed pulps were covered with mineral trioxide aggregate (ProRoot). The pulpal bases were all covered with a RMGI (Fuji II LC). The tissue samples were collected at one day, two days, one week and over four weeks (34 days). Results: Following perfusion fixation, the samples were demineralized, sectioned, stained and histologically graded. After histologic analysis, presence of neutrophilic infiltrate and areas of hemorrhage with hyperemia were observed . The depth of the neutrophilic infiltrate depended on the agent or material used. The pupal tissue treated with Otic suspensions demonstrated significantly less inflammation (Kruskal Wallis non parametric analysis, H=9.595 with 1 degree of freedom; P=0.0223) than the formocresol and control groups. The hard tissue bridges formed over the exposure sites were more organized in the MTA treatment groups than in the control and ZOE groups (Kruskal Wallis non parametric analysis, H=18.291 with 1 degree of freedom; P=0.0004). Conclusions: Otic suspensions and MTA are effective in treating bacterial infected pulps and stimulate the production of a hard tissue bridge over the site of the exposure.

Uso de la inmunohistoquímica como herramienta epidemiológica para el diagnóstico de rabia bovina a partir de casos no conclusivos

Objective. Identify the rabies virus in cases of nervous disease archived in the laboratory with diagnosis of nonspecific encephalitis. Materials and methods. Twelve samples of bovine brain suspected of rabies, were processed by indirect immunoperoxidase technique using polyclonal antibodies against the viral agent. Results. Was demonstrated the presence of viral antigens in three cases in the form of small aggregates in the cytoplasm of neurons, with a pattern of round or oval and a variable number of viral inclusion bodies. We discussed the importance of the results in Colombia, the usefulness of the technique in the difficult conditions for sending samples to the laboratory, plus the possible relationship of the negative cases with bovine herpesvirus 5. Conclusions. The use of immunohistochemical technique to demonstrate rabies virus antigens in formalin fixed bovines tissues can help in the construction of an epidemiological map of rabies disease in Colombia and may reduce the high under-diagnosis of diseases of the nervous system of cattle, reported in some regions.

Factors affecting somatic cell counts and their relations with milk and milk constituent yield in buffaloes

Data concerning daily milk yield (MY), percentage of milk fat (%F), protein (%P), lactose (%LT), and total solids (%TS), and somatic cell counts (SCC) for a herd of 222 Murrah buffalo reared in the state of São Paulo, Brazil, were collected monthly from 1997 to 2000 in order to study the factors affecting SCC and their relation to milk production and constituents during lactation. SCC decreased in the second month of lactation and increased thereafter, up to the ninth month of lactation. The interaction of month of lactation x order of calving was significant. Mean MY observed during the first month of lactation was 6.87 kg, which increased to 7.65 kg during the second month, and then decreased until the ninth month of lactation (3.83 kg). During the different months of lactation, %F, %P, %LT, and %TS ranged from 6.28 to 8.38%, 4.05 to 4.59%, 4.96 to 5.34%, and 16.94 to 18.55%, respectively. Calving year, calving order, and order of month of lactation significantly affected MY, %F, %P, %LT, and %TS. The regression coefficients of transformed SCC on MY and %LT were negative and significant during all months of lactation, showing that milk and lactose yield decreased with increased transformed SCC, causing losses to buffalo milk producers.

Adsorption of polyelectrolytes onto oppositely charged cylindrical macroions

In this work we investigate the adsorption of polyelectrolyte chains onto uniformly charged cylindrical macroions by means of the Metropolis Monte Carlo simulations and weighted histogram analysis method. Adopting a simplified model for macromolecules and treating the electrolytic solution in the Debye-Hückel level, conformational properties of the adsorbed chain, such as the radius of gyration and the thickness of the adsorbed layer, are provided as a function of ionic strength and macroion charge density. By analysis of the free energy profile as a function of the radius of gyration it was possible to identify first-order-like transitions between adsorbed and desorbed states and obtain a macroion charge density dependence of the critical ionic strength in good agreement with experiments. © 2013 AIP Publishing LLC.

Cidades pequenas e indústria: contribuição para a análise da dinâmica econômica na região de Presidente Prudente-SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo dos aspectos eletrostáticos da interação entre polieletrólitos e macroíons

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tradição narrativa e a ação cotidiana na explicitação de atos de fala em narrativas de escolares

Discutem-se condições nas quais se dá a explicitação de atos de fala em narrativas produzidas por alunos de 5a série do 1B grau. Além disso, apresentam-se sugestões para o trabalho com atos de fala em narrativas.

Indícios de subjetividade na fala de uma criança psicótica: um olhar para as hesitações

This study, performed under a linguistic-discoursive prism, aimed to: (1) describe the moments in which occurred hesitations in the utterances of a psychotic child diagnosed with Language Disorder; and (2) determine the extent to which these moments (also) can indicate aspects of a subjectivity trying to emerge in this child. Data were extracted from a speech therapy session with a ten year old female child. Concerning the fi rst goal, from a total of 362 utterances produced by the child, only 74 (20%) had hesitation traces, while 288 (80%) did not. Concerning the second goal, the utterances with hesitation traces occurred in situations of: topic development, especially in the form of complementarity; introduction of new topic; return to the previous topic; refuses to the topic; enunciative incompleteness. The high percentage of utterances without hesitation traces (80%) is explained because they are highly predictable from the context, mostly in situations of ritualized adjacent pairs, oftentimes in situations of immediate specularity. The reduced percentage of utterances with hesitation traces are explained precisely by the fact that, unlike those without traces, in these ones, signs of a subjectivity that tries to emerge and show itself in the discourse production are detected. With the development of this study, we tried to emphasize the view at the hesitations as marks of subjectivity – in other words, evidences of confl icting relationships between the subject and the others that constitute the utter. The concern was also about bringing to the fi eld of Speech Pathology discoursive linguistic refl ections based on data extracted from symptomatic contexts of language.

Distribuição de pausas na interpretação de atores

Introduction: The literature about the work with the voice of actors in the theater focuses the research on organic issues involved in the vocal process as “misuse” or “vocal abuse”. To a lesser amount, there are studies that highlight issues of interpretation and expressive resources, as well as others that highlight the linguistic resources of interpretation. This work shows a linguistic feature – the pause – and its collaboration for the theatrical interpretation. Objective: to verify the extent to which similarities and differences occur between the points judged as occurrence of pauses in interpretations of a theatrical text. Method: Subjects of this study were four theater actors, who played individually a same theatrical text. Later, a group of ten judges judged the points where pauses occurred. Based on a criterion of 70% agreement between judges, were searched similarities and differences between the points judged as pause of each recording. Results: the four subjects showed different amounts of pause in the stretch in common that they interpreted: S1= ten occurrences; S2= 14 occurrences; S3= 6 occurrences; and S4= 9 occurrences. In this inter-subject variation, five points of pause in common were detected. Conclusion: we have seen, then, that this variability (featuring subjectivity in interpretation) is built inside given possibilities, with lesser or greater flexibility, by the structure of the theatrical text.

Aquisição da linguagem: as crianças e sua atitude responsiva a perguntas abertas

Introduction: Since their first month of life, babies already show alternation in their communication, in which adults have an important role, assuming interaction turns with the child through questions known as eliciting questions. Verifying this alternation incited us to analyze children’s responsive attitudes toward the questions of the adult interlocutor. Objetives: (1) describe and characterize the kinds of responsive attitudes children have to open questions; (2) verify if there are any differences between the developed and non-developed kinds in the answers. Material and method: data were extracted from 28 interviews (recorded both in audio and in video) with four male children (5-6 years-old) who attended a public Kindergarten. Results: regarding the first objective, 88.7% of the attitudes were answers to the questions, 4.7% were non-responses and 6.6% were confirmation requests. Regarding the second objective, 48.2% of the answers were developed and 51.3%, non-developed. Conclusion: Although the high percentage of answers indicates that the children showed themselves sensitive to the demands of the adult interlocutor, the small percentage difference between developed and non-developed answers also indicates that children mostly depend on their assistance to develop their utterance since they oscillate between restricting themselves to the demand of the interlocutor and expanding it.

Pausas na interpretação teatral: delimitação de constituintes prosódicos

We intend to observe the function of a linguistic resource – the pause – in theatrical interpretation. Connected to the field of speech therapy, we search for theoretical support in the Linguistics field, mainly in prosodic phonology – specifically, we highlight intonational phrase and phonological utterance, prosodic constituents –, proposing a dialogue between these fields, regarding the work with actors. In speech therapy literature, the work with actors focuses, centrally, in organic issues involved in the vocal process, such as “misuse” or “voice abuse”. To a smaller extent, we find, in this literature, researches that emphasize issues regarding interpretation and expressive resources, besides a few emphasizing the importance of linguistic resources in interpretation. Differently, in linguistics literature, the pause is approached, to a larger extent, from the phonetic perspective, related to several language levels. In this research, we analyzed audio recordings of four actors from a same theatrical group, acting the theatrical text Brutas flores, focused on these aims: (1) detect the place where pauses happen in the interpretation of a single text by four actors; (2) survey physical characteristics of length of these pauses; (3) check to what extent the length of a pause is related to the place where it happens, regarding the prosodic limits of intonational phrases (I) and phonological utterance (U). We could observe that, although the interpretation is characterized by the subjectivity of the actor, the interpretation is constructed based in the possibilities offered by the prosodic organization of the text itself, being more or less flexible.We were also able to confirm, by considering the length of VVs units containing pauses, the prosodic hierarchy proposed by Nespor & Vogel, once the length of these units in U's limits was significantly higher than the length in I's limits. Thus, our results reinforce the premise that a linguistic structure overlaps the subjectivity of the actor, i. e., the premise that the strength of linguistic structure organization acts on the possible individual operation/style.

Flutuação na segmentação de palavras: relações entre constituintes prosódicos e convenções ortográficas na escrita infantil

In this paper, we analyzed situations where (a) one vocabular structure showed in two different ways in the same text or (b) had erasures. These structures fluctuations were extracted from texts written by children that, when the registers were done, were second graders of elementary school. Concerning the results, we verified: (1) that more than one prosodic constituent showed in the basis of fluctuation of vocabulary structures; and (2) that at least one of the limits of orthographic words was maintained in fluctuation structures. These results point to the recuperation done by writers with information they have access due to their insertion in (1) oral practices and (2) literacy practices.