Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa

The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival.

Restauración onlay usando técnica semi-directa con resina compuesta: reporte de un caso

Posterior teeth restorations have changed the contemporary treatment philosophy influenced by the aesthetic demand of patients, progress of adhesive material science and techniques for preservation and rehabilitation of affected teeth by dental caries and trauma. The development of Onlay restorations with semi-direct technique in endodontically teeth treated aims to preserve the remaining surfaces, to reduce the possibility of fracture and polymerization shrinkage. In addition, better restoration adaptation and marginal seal, resistance to wear and dimensional stability are achieved. This case reports the rehabilitation of an endodontically treated permanent maxillary first molar in a 13 years old- patient who attended the Araraquara School of Dentistry, Brazil, using Miris 2 Composite resin with semi-direct technique and obtaining an aesthetic and functional restoration in a single appointment. The fundaments and clinical guidelines of the procedure are detailed, based on the review of the literature that supports this conservative treatment.