Production of transgalactosylated oligosaccharides (TOS) by galactosyltransferase activity from Penicillium simplicissimum

Ingestion of transgalactosylated oligosaccharides (TOS) and other non-digestible oligosaccharides (NDOs) induces a significant increase in Bifidobacterium, Lactobacillus and some desirable species of Streptococcus populations in the gut of human and other animals (prebiotic effect). This change in the intestinal flora is responsible for several beneficial physiological effects such as a decrease of putrefactive products in the feces, lower blood cholesterol content, higher Ca2+ absorption, a smaller loss of bone tissue in ovariotomized female rats and a lower incidence of colon cancer. beta-Galactosidase from Penicillium simplicissimum, a strain isolated from soil, showed high galactosyltransferase activity when incubated with a highly concentrated lactose solution. Optimum pH and temperature ranges for hydrolytic activity were 4.0-4.6 and 55-60 degrees C, respectively, for a lactose concentration of 5.0% (w/v). Maximal galactosyltransferase activity was obtained at pH 6.5 and 50 degrees C and TOS synthesis was positively associated with lactose concentration in the reaction medium. Thus, when 50 ml of a 60% (w/v) lactose solution was incubated with 26.6 U of beta-galactosidase under the best pH and temperature conditions for transferase activity, a final product with 30.5% TOS (183 mg ml(-1)), 27.5% residual lactose and 42.0% monosaccharides was obtained. (C) 1999 Elsevier B.V. Ltd. All rights reserved.

Properties of a new fungal b-galactosidase with potential application in the dairy industry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Purification and properties of an alkaline protease of Aspergillus clavatus

An extracellular alkaline serine protease has been purified from a strain of Aspergillus clavatus, to apparent homogeneity, by ammonium sulfate precipitation and chromatography on Sephadex G-75. Its molar mass, estimated by SDS-PAGE, was 35 kDa. Maximum protease activity was observed at pH 9.5 and 40 degrees C. The enzyme was active between pH 6.0 and 11.0 and was found to be unstable up to 50 degrees C. Calcium at 5 mM increased its thermal stability. The protease was strongly inhibited by PMSF and chymostatin as well as by SDS, Tween 80 and carbonate ion. Substrate specificity was observed with N-p-Tos-Gly-Pro-Arg-p-nitroanilide and N-Suc-Ala-Ala-Ala-p-nitroanilide being active substates. Parts of the amino acid sequence were up to 81% homologous with those of several fungal alkaline serine proteases.

Síndrome do desfiladeiro torácico - ressecção de costela cervical por videotoracoscopia

A base do tratamento cirúrgico da Síndrome do Desfiladeiro Torácico (SDT) é a ressecção da primeira costela, podendo associar-se à escalenectomia ou ainda à ressecção de costela cervical. Esta última é feita tradicionalmente por meio de um acesso supraclavicular ou mesmo axilar, o qual é tecnicamente mais trabalhoso. Pode ser realizada também por meio de acesso paraescapular. Embora tecnicamente atrativa e associada à menor invasividade e maior segurança, com ótimo resultado estético, a ressecção da primeira costela torácica, por intermédio de cirurgia videoassistida transaxilar ou pela técnica videotoracoscópica, é pouco relatada na literatura, e nenhuma referência foi encontrada sobre ressecção de costela cervical mediante essa técnica. Neste artigo, apresentamos essa inovação cirúrgica realizada com sucesso para ressecção de costela cervical em duas pacientes.

Alocação ótima de reguladores de tensão em sistemas de distribuição de energia elétrica radiais usando uma formulação linear inteira mista. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de similaridades e diferenças no uso de marcadores de reformulação e padrões lexicais em Family ties, The apple in the dark e Soulstorm, de Clarice Lispector, e The red house, de Lya Luft

Pós-graduação em Estudos Linguísticos - IBILCE

Fontes de lipídios na reprodução e larvicultura de Tilápia-do-Nilo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise de similaridades e diferenças no uso de marcadores de reformulação e frases lexicais, em um corpus paralelo, constituído de contos e romances de Claice Lispector, e as respectivas traduções para o inglês e italiano

Pós-graduação em Estudos Linguísticos - IBILCE

Interações tróficas entre espécies pelágicas do Atlântico Sudoeste: utilizando isótopos estáveis e inferência bayesiana

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Análise dos níveis de motivação e estresse em atletas do campeonato regional de basquetebol feminino sub-21

This descriptive study aimed to investigate what changes of emotional states more sensitive to internal and external influences and to investigate the motivating factors and stressors in basketball players of a Regional Championship of the Interior . Study subjects were 56 (fifty - six) athletes, members of 05 (five) highest ranked teams in the competition. As a measuring tool used the Frester ‟sP sychicLoad Test (1972, apud SAMULSKI, CHAGAS, 1992) . Data analys used descriptive statistics. The findings showed that the facts considered as motivators during the competition were: the audience, the fact of constantly thinking on achieving the desirable aims, and the establishment of very high aims; the stressful factors were: disagreements and conflicts with the coach, partners or family, bad sleeping the night before the competition, excessive nervousness and to be prejudiced by the referees . Therefore, we can conclude: the facts that influence the interest can be of motivating or stressful order , depending on the personal and situational perception; most athletes characterized the competiti ve sport as more stressful than motivating, and this assessment influenced by constant pressure of intrinsic and extrinsic factors that athletes suffers during the competitive environment

Desempenho em tarefas computacionais em função do uso de diferentes periféricos

With the popularization of information technology and government incentives for inclusion in the digital era, an important issue for science and for industry is the design of tool s for entrance of input to the device, that is , the peripherals at tached to the computer that are reaching the consumer. In order to minimize the impact of repetitive use of these tools for entry, whether when playing games or working, more studies are in need to developper ipherals that enable comfort and effi ciency. This study examined the per formance of individuals with differents izes of hand (i.e., large and small ) in performance of three task s on the computer us ing mouses of small and large sizes. For thi s study, 19 young adul ts were volunteers and asked to per form activities with greater precision and speed. In addition, al l participants were asked on the use of computers. The results showed no statistically significant differences in the performance related to the size of the mouse between subjects with small and large hands. However, an interest ingresult as observed with respect to the performance of individuals with a large hand to perform tas ks more quickly. The results of this study suggest that with both, the small and the large mouse, individuals with big hands have a fas terper formancein tasks of dragging and clicking when compared with the performance of indi viduals with small hand.

Projeto de controlador gain scheduling usando realimentação derivativa via LMI

Pós-graduação em Engenharia Elétrica - FEIS

Deteccão de complexos QRS em eletrocardiogramas baseada na decomposição em valores singulares em multirresolução

Pós-graduação em Engenharia Elétrica - FEIS