A molecular mechanism for Lys(49)-phospholipase A(2) activity based on ligand-induced conformational change

Agkistrodon contortrix laticinctus myotoxin is a Lys(49)- phospholipase A(2) (EC 3.1.1.4) isolated from the venom of the serpent A contortrix laticinctus (broad-banded copperhead). We present here three monomeric crystal structures of the myotoxin, obtained under different crystallization conditions. The three forms present notable structural differences and reveal that the presence of a ligand in the active site (naturally presumed to be a fatty acid) induces the exposure of a hydrophobic surface (the hydrophobic knuckle) toward the C terminus. The knuckle in A contortrix laticinctus myotoxin involves the side chains of Phe(121) and Phe(124) and is a consequence of the formation of a canonical structure for the main chain within the region of residues 118-125. Comparison with other Lys(49)-phospholipase A(2) myotoxins shows that although the knuckle is a generic structural motif common to all members of the family, it is not readily recognizable by simple sequence analyses. An activation mechanism is proposed that relates fatty acid retention at the active site to conformational changes within the C-terminal region, a part of the molecule that has long been associated with Ca2+-independent membrane damaging activity and myotoxicity. This provides, for the first time, a direct structural connection between the phospholipase active site and the C-terminal myotoxic site, justifying the otherwise enigmatic conservation of the residues of the former in supposedly catalytically inactive molecules.

Expressando-se: uma interface do ensino de artes visuais com a musicalidade

Pós-graduação em Artes - IA

O brinquedo do parque: um conceito lúdico como arte do reaproveitamento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O culto da deusa Ísis entre os romanos no século II: representações nas Metamorfoses de Apuleio

Pós-graduação em História - FCLAS

Comparação entre sutura e cola de fibrina derivada do veneno de serpente para fixação de enxerto de tecido conjuntivo na correção de recessões teciduais marginais

Pós-graduação em Bases Gerais da Cirurgia - FMB

Religião e silêncio: representações e práticas mortuárias entre nikkeis em Assaí por meio de túmulos (1932 – 1950)

Pós-graduação em História - FCLAS

Tempo de festas: homenagens a Santa Bárbara, N. S. da Conceição e Sant'Ana em Salvador (1860-1940)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O SLA funk de Fernanda Abreu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Um olhar sócio-histórico sobre a beleza: das amarras à alteridade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Religião Romana nas Fronteiras da Romanidade: um estudo sobre a construção e manutenção de identidades romanas durante o principado Júlio-Claudiano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

São Paulo 2012: Um sonho, um projeto, uma visão

Millennia ago, through the myths of greeks gods and demi-gods, comes a series of celebrations called Pan-Hellenic Games. Among these festivals, the most famous was certainly the Olympic Games - the Olympics. Forbidden by Rome in the third century AD, they are considered to worship the pagan gods, were rescued in 1894 by Pierre de Coubertin. Over 113 years and 29 Olympiads, the event took gigantic proportions and the right to host the Games has become one of the closest racing of the modern world. Catalyst for urban development, the Olympic Games were the subject of dream for the city of Sao Paulo in the 2000’s, and an internal dispute was fought with Rio de Janeiro to get the right to host the 2012 Games. A team of Paulo Mendes da Rocha and associates was responsible for designing the plan. Eliminated from contention, Sao Paulo forgot the Olympic dream, and in October 2009 the city of Rio was finally chosen to host the Olympics in 2016. This project aims to rescue the look of architectural and urban plan Sao Paulo 2012 and with a critical and poetic eye create a visual identity for a fictitious victory in the election of São Paulo in 2003. Not aiming to create a corporate identity program - because this is not the mission of the architect - but look at Sao Paulo and the Olympics with poetry and criticism in the true Olympic spirit of peace and hope.

As Virgens Marias da Nova Espanha e do Arcebispado de Lima durante os séculos XVI e XVII: entre a idolatria e a santidade

Pós-graduação em História - FCHS

Corporalidade neopentecostal: análise e concepção de corpo na Igreja Bola de Neve de Marília, SP

Pós-graduação em Ciências Sociais - FFC

Semana Ilustrada, o Moleque e o Dr. Semana: imprensa, cidade e humor no Rio de Janeiro do 2º Reinado

Pós-graduação em História - FCLAS

A linguagem do consenso nos marcos da regressão cultural: o fim da consciência e a construção do neocontratualismo de Jürgen Habermas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)