Atributos físicos nos tempos de adoção de manejos em Latossolo cultivado com soja

A condução das operações de preparo de forma inadequada ocasiona sérios problemas de conservação do solo, destacando-se a compactação, que acarreta a redução do espaço poroso, principalmente dos macroporos, e altera os atributos físico-hídricos. Este trabalho teve como objetivo verificar a influência dos diferentes sistemas e tempos de adoção de manejos em Latossolo Vermelho de Jaboticabal, Estado de São Paulo, por meio da densidade máxima, e correlacioná-la com a produtividade da soja, a densidade relativa e a umidade crítica de compactação. O delineamento experimental foi o inteiramente casualizado com parcelas subdivididas (cinco sistemas de uso e três camadas), com quatro repetições. Os cinco sistemas de uso foram: plantio direto por cino anos (SPD5), plantio direto por sete anos (SPD7), plantio direto por nove anos (SPD9), preparo convencional (SPC) e uma área adjacente de mata nativa (MN). As camadas do solo avaliadas foram as de 0-0,10, 0,10-0,20 e 0,20-0,30 m, nas quais foram determinados a densidade máxima do solo (Ds máx), a umidade crítica de compactação (Ugc), a densidade relativa do solo (Dsr), a composição granulométrica, a porosidade e o teor de matéria orgânica do solo. Os resultados mostraram que o comportamento das curvas de compactação do solo foi o mesmo em todas as camadas dos diferentes manejos e que os teores de matéria orgânica não justificaram as pequenas alterações da Ds máx. Para o Latossolo Vermelho, as operações mecanizadas nos sistemas de manejo podem ser executadas na faixa de 0,13 a 0,19 kg kg-1 de umidade sem causar degradação física. Verificou-se que a Dsr ótima e a umidade crítica de compactação foram de 0,86 e 0,15 kg kg-1, respectivamente, embora os diferentes sistemas e tempos de adoção de manejo tenham apresentado comportamento semelhante quanto à produtividade da soja.

Alterações nos atributos químicos de um Latossolo distroférrico decorrentes da granulometria e doses de calcário em sistemas plantio direto e convencional

Atualmente, percebe-se o interesse na calagem superficial, sem prévia incorporação, para instalação do sistema plantio direto (SPD). Dessa forma, objetivou-se determinar os efeitos de granulometria e doses de calcários no SPD, em fase de implantação, e no sistema de plantio convencional (SPC) sobre o pH, H + Al, Ca2+ e Mg2+. O experimento foi realizado no ano agrícola de 1998/99, na FCA/UNESP-Botucatu (SP), em Latossolo Vermelho. O delineamento experimental foi de blocos ao acaso, com parcelas subsubdivididas e quatro repetições. As parcelas representaram os sistemas de plantio (SPD e SPC); as subparcelas, a granulometria dos calcários [grosso (PRNT = 56 %) e fino (PRNT = 90 %)], e as subsubparcelas, as doses de 2, 4 e 6 t ha-1 (calcário grosso) e 1,2; 2,4 e 3,6 t ha-1 (fino). O solo foi amostrado, a 0-5, 5-10, 10-20 e 20-40 cm de profundidade, 1, 3 e 12 meses após a aplicação dos corretivos. A análise de variância não detectou interação tripla entre os fatores. A aplicação de calcário superficial no SPD, independentemente da granulometria e da dose, alterou positivamente os atributos químicos do solo (0-5 e 5-10 cm), 12 meses após a calagem. O corretivo continuou reagindo, independentemente do sistema de plantio, de forma intensa, mesmo após três meses. A aplicação de doses mais elevadas de calcário, com maior granulometria, sugeriu efeito residual prolongado.

Study of the diclofenac/phospholipid interactions with liposomes and monolayers

The interaction of diclofenae sodium (SD) with soya phosphatidylcholine (SPC) has been studied with floating Langmuir monolayers and liposomes. SD was either introduced into the subphase of SPC monolayers or co-spread with SPC on an aqueous subphase. In both cases, SD caused the surface pressure isotherm to become more expanded, thus demonstrating the affinity between SD and SPC. The incorporation of SD caused SPC liposomes to have a decreased diameter according to light scattering experiments. When SPC liposomes were injected into an aqueous subphase, their destruction yielding surface-active monomers could be monitored by changes in surface pressure. SD-loaded liposomes displayed a much faster kinetics when the surface density of surface-active monomers was plotted against time, with rate constants increasing significantly with the SD concentration. The kinetic profile can be quantitatively analyzed by plotting In[1 - (Gamma/Gamma(infinity))] versus t(1/2) (C) 2004 Elsevier B.V. All rights reserved.

Structure and viscoelastic behavior of pharmaceutical biocompatible anionic microemulsions containing the antitumoral drug compound doxorubicin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural Health Monitoring in Smart Structures Through Time Series Analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fracture strength of incisor crowns after intracoronal bleaching with sodium percarbonate

Objectives: To compare the fracture resistance of bovine teeth after intracoronal bleaching with sodium percarbonate (SPC) or sodium perborate (SP) mixed with water or 20% hydrogen peroxide (HP). Materials and methods: Fifty extracted bovine teeth were divided into four experimental groups (G1G4) and one control (n = 10) after endodontic treatment. Following root canal obturation, a glass ionomer barrier was placed at the cementoenamel junction. After that, the pulp chambers were filled with: G1 SP with water; G2 SP with 20% HP; G3 SPC with water; and G4 SPC with 20% HP. No bleaching agent was used in the control group. Coronal access cavities were sealed with glass ionomer and specimens were immersed in artificial saliva. The bleaching agents were replaced after 7 days, and teeth were kept in artificial saliva for an additional 7 days, after which the pastes were removed and the coronal access cavities were restored with glass ionomer. Crowns were subjected to compressive load at a cross head speed of 0.5 mm min-1 applied at 135 degrees to the long axis of the root by an EMIC DL2000 testing machine, until coronal fracture. Data were statistically analysed by anova and Tukey test. Results: No differences in fracture resistance were observed between the experimental groups (P > 0.05). However, all experimental groups presented lower fracture resistance than the control group (P < 0.05). Conclusion: SPC and SP led to equal reduction on fracture resistance of dental crowns, regardless of being mixed with water or 20% HP.

Exotic snakes in São Paulo City, southeastern Brazil: Why xenophobia ?

Introduced exotic species cause environmental changes and threat public health in target sites. Illegal trade has enhanced this problem. To first report these risks in Brazil, exotic snakes found in São Paulo City (SPC) (23degrees32' S, 46degrees38' W), southeastern Brazil, and sent to Instituto Butantan between 1995 and 2000, were listed and characterized by their biological attributes. Seventy-six individuals of sixteen alien species were collected. Euriecians snakes, mainly booids, were predominant. Using multivariate techniques, their ecological niches were compared to those of 26 native species, as a way to point out the resource's availability. To evaluate the potential of successful implantation, two species absent in SPC and considered as problem snakes are included in these analyses: the brown treesnake Boiga irregularis and the habu Trimeresurus flavoviridis. There were niche similarities between these pest snakes, exotic booids and native viperids largely due to the similarities in the chosen prey (mammals), diel activity (nocturnal), color pattern (variegated) and body size (medium to large). To avoid predictable undesirable effects of implanted pest snakes, traffic control and punishment should be improved, as well as parallel environmental education programs.

Doxorubicin biocompatible O/W microemulsion stabilized by mixed surfactant containing soya phosphatidylcholine

Microemulsions (ME) containing soya phosphatidylcholine (SPC/polyoxyethylenglycerol trihydroxystearate 40 (EU)/sodium oleate (SO) as surfactant cholesterol (CHO) as oil phase and aqueous buffer were studied. Pseudo-ternary phase diagrams of the investigated systems were obtained at constant SPC/EU/SO weight ratio 3.5:3.5:3.0 by titration, in order to characterize the proportions between the components to form clear systems. The dynamic light scattering results showed that the size of the oil droplets decreases significantly with the ratio of surfactant/oil phase added to system. Depending on the composition ME system could exhibit a thixotropic behavior. The apparent viscosity increased 25- and 13-folds with cholesterol concentration for drug-free and drug-load ME, respectively. It was also verified that the octanol/aqueous buffer partition coefficient (K-O/B) of doxorubicin (DOX) was pH dependent increasing abruptly above pH 6.0. It was possible to incorporate 2.24 mg/ml of DOX into ME. The incorporation of DOX in the ME systems increased the droplets size for all surfactant concentrations used in the system. The results suggest that DOX interacts with the microstructure of the ME at the studied pH increasing significantly the drug solubility. It was possible to conclude that the investigated ME can be a very promising vehicle as drug-carrier for administration of doxorubicin. (c) 2006 Elsevier B.V. All rights reserved.

Relationship between structural features and in vitro release of doxorubicin from biocompatible anionic microemulsion

In this work structural features of anionic microemulsions, containing the pharmaceutical biocompatible components soya phosphatidylcholine (SPC), eumulgin HRE 40 (EU) and sodium oleate (SO) as surfactant, cholesterol (CHO) as oil phase and aqueous buffer were studied. Microemulsions were formulated with and without the antitumor drug doxorubicin (DOX). The various microstructures characterized in the pseudo-temary phase diagram were analyzed by polarized light microscopy, small-angle X-ray scattering (SAXS) and X-ray diffraction (XRD) as well as by their ability to incorporate and release DOX. The experimental results demonstrated a correlation between the composition, the structural features and drug delivery. It was found that at higher cholesterol contents, the crystallization of CHO polymorph phases changed the mobility of DOX molecules. Droplets were formed with short-range spatial correlation from a microemulsion (ME) with a low surfactant:oil ratio. More ordered structures with lamellar arrangements formed by the increasing of the CHO proportions in the formulation may be due to CHO crystallization. The in vitro release of DOX showed that the presence of a high content of crystalline CHO prolongs the release of DOX from ME. The retention of DOX in the internal oil phase of the ME may modulate the drug release for a prolonged time. These results clearly demonstrate the potential of ME as a drug-delivery system. (c) 2007 Elsevier B.V. All rights reserved.

Structural changes of biocompatible neutral microemulsions stabilized by mixed surfactant containing soya phosphatidylcholine and their relationship with doxorubicin release

Depending on the composition, the mixture of surfactant, oil and water, may form supramolecular aggregates with different structures which can significantly influence the drug release. In this work several microemulsion (ME) systems containing soya phosphatidylcholine (SPC) and eumulgin HRE40 (TM) (EU) as surfactant, cholesterol (O) as oil phase, and ultra-pure water as an aqueous phase were studied. MEs with and without the antitumoral drug doxorubicin (DOX) were prepared. The microstructures of the systems were characterized by photon correlation spectroscopy, rheological behavior, polarized light microscopy, small-angle X-ray scattering (SAXS) and X-ray diffraction (XRD). The results reveal that the diameter of the oil droplets was dependent on the surfactant (S) amount added to formulations. The apparent viscosity was dependent on the O/S ratio. High O/S ratio leads to the crystallization of cholesterol polymorphs phases which restricts the mobility of the DOX molecules into the ME structure. Droplets with short-range spatial correlation were formed from the ME with the low O/S ratio. The increase of the cholesterol fraction in the O/S mixture leads to the formation of ordered structures with lamellar arrangements. These different structural organizations directly influenced the drug release profiles. The in vitro release assay showed that the increase of the O/S ratio in the formulations inhibited the constant rate of DOX release. Since the DOX release ratio was directly dependent on the ratio of O/S following an exponential decay profile, this feature can be used to control the DOX release from the ME formulations. (C) 2008 Elsevier B.V. All rights reserved.

Qualidade da colheita mecanizada de feijão (Phaseolus vulgaris) em dois sistemas de preparo do solo

Dentre as etapas de produção do feijoeiro a colheita é uma das mais importantes, porque pode interferir de maneira decisiva na qualidade e no custo de produção. Assim, objetivou-se avaliar a qualidade da operação da colheita mecanizada de feijão (Phaseolus vulgaris), cultivado sob preparo convencional e plantio direto. As variáveis analisadas foram: o nível de ruído emitido, calculado através de um medidor de pressão sonora; o desempenho operacional, sendo monitorado o consumo de combustível, a patinagem dos rodados e a velocidade de deslocamento do conjunto coletados em uma central digital (datalogger); e a operação de colheita quanto à matéria seca e densidade de palhada, e as perdas na colheita. A velocidade e os consumos horário e operacional apresentaram distribuição normal dos dados, enquanto que o nível de ruído apresentou distribuição assimétrica. As perdas na colheita mecanizada de feijão e a densidade de palhada apresentaram baixa variabilidade e distribuição normal. Assim, apenas o consumo horário e a produção de matéria seca de palhada apresentaram comportamento instável em relação ao controle estatístico de processo, enquanto os demais indicadores mostraram condições de manter a qualidade da operação de colheita tanto no preparo convencional de solo quanto no plantio direto.

Quality of mechanized coffee harvesting in circular planting system

A colheita mecanizada de café se destaca como um dos principais avanços tecnológicos em benefício dos produtores, porém, problemas com planejamento no plantio e desenvolvimento da cultura, bem como falhas na regulagem da colhedora, podem acarretar em redução na eficiência da operação, tornando-a mais estressante para a cultura que a colheita manual. Diante disso, o presente trabalho foi realizado no município de Patos de Minas, MG, com o objetivo de avaliar os danos na parte vegetativa e a qualidade da colheita mecanizada do café cultivado em plantio circular sob pivô central, com duas frequências de vibração das hastes, sob o controle estatístico do processo. Observou-se que a bienalidade negativa sobre a cultura reduziram a carga inicial de café, eliminando o efeito das condições de insolação. Os índices de qualidade da colheita apresentaram distribuição assimétrica, com grande variabilidade nas amostras, sendo apenas a eficiência de derriça influenciada pelos eixos avaliados, e se mostrando estável pelo controle estatístico. Os danos causados às plantas pela colheita apresentaram valores desejáveis, não variando em função dos fatores analisados, mas sendo considerado sob controle estatístico.

Biossorção de cobre, manganês e cádmio por biomassas de Saprolegnia subterranea(Dissmann) R.L. Seym. e Pythium torulosumCoker & P. Patt. (Oomycetes)

Dried biomass of the zoosporic fungi Saprolegnia subterranea and Pythium torulosum was evaluated for copper, manganese and cadmium biosorption from aqueous solutions using the "q" (mg of adsorbed metal per g of biomass) and the "R%" (percent removal) indices. The highest q values were observed when the biomass was placed in contact with high metal concentrations, whereas the highest R% values were observed at low concentrations (p< 0.05). S. subterranea SPC 1244 biomass surpassed the others for copper biosorption (q = 7.48 mg/ g; R% = 49.03), P. torulosum SPC 1425 biomass was the best for manganese biosorption (q = 4.13 mg/g; R% = 26.71), and S. subterranea SPC 1431 biomass was the best for cadmium biosorption (q = 6.75 mg/g; R% = 42.26). This is the first report on copper, manganese and cadmium biosorption by the biomass of these zoosporic fungi, indicating the potential to remove ions from diluted solutions.

Passive Immunity of Progeny from Broiler Breeders Vaccinated with Oil-Emulsion Bacterin Against Salmonella Enteritidis

Young poultry are very susceptible to Salmonella Enteritidis (SE) infections because of the absence of complete intestinal flora colonization and an immature immune system. This study evaluated the role of passive immunity on the resistance of young birds against early infections caused by SE. The progeny of broiler breeders vaccinated with an oil-emulsion bacterin was compared to the progeny of unvaccinated birds. Efficacy was determined by challenging birds at 1 and 14 days of age with SE Nal Spc strain, phage type 4. After challenge at 1 day of age, the progeny of vaccinated birds presented a significantly lower number (log(10)) of SE Nal Spc reisolation (P < 0.05) in liver (2.21), spleen (2.31), and cecal contents (2.85) compared with control groups (2.76, 3.02, and 6.03, respectively). The examination of the internal organs, 3 days after infection, revealed that 28% of the birds (7/25) from vaccinated breeders were positive, whereas 100% (25/25) of the chicks derived from unvaccinated birds were positive. Birds challenged at 14 days of age presented a lower number of positive samples compared with those challenged at 1 day of age, and the progeny of vaccinated birds presented statistically lower numbers (log(10)) of colony-forming units/ml of SE Nal Spc only in the cecal contents compared with nonvaccinated breeder progeny (2.11 vs. 2.94). Age seems to influence the susceptibility of birds to SE infections: in control groups, the number of positive birds at 14 days of age (9/25) was lower when compared with the group infected at 1 day of age (25/25). The number of positive fecal samples of the progeny of vaccinated birds was significantly lower (36) than those of the control group (108) after challenge at 1 day of age. Unchallenged progeny of vaccinated birds presented passive antibodies detectable by enzyme-linked immunosorbent assay (ELISA) up to 21 days of age. on the other hand, antibodies of the control group were detected by ELISA 14 days after challenge. These results show a significant contribution of breeder vaccination by increasing the resistance of the progeny against early SE infections. However, the bacteria were not completely eliminated, suggesting that additional procedures are needed to effectively control SE infections.