13 resultados para Roseau commun

em Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho"


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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SMase I, a 32 kDa sphingomyelinase found in Loxosceles laeta venom, is responsible for the major pathological effects of spider envenomation. This toxin has been cloned and functionally expressed as a fusion protein containing a 6 x His tag at its N-terminus to yield a 33 kDa protein [Fernandes-Pedrosa et al. (2002), Biochem. Biophys. Res. Commun. 298, 638 - 645]. The recombinant protein possesses all the biological properties ascribed to the whole L. laeta venom, including dermonecrotic and complement-dependent haemolytic activities. Dynamic light-scattering experiments conducted at 291 K demonstrate that the sample possesses a monomodal distribution, with a hydrodynamic radius of 3.57 nm. L. laeta SMase I was crystallized by the hanging-drop vapour-diffusion technique using the sparse-matrix method. Single crystals were obtained using a buffer solution consisting of 0.08 M HEPES and 0.9 M trisodium citrate, which was titrated to pH 7.5 using 0.25 M sodium hydroxide. Complete three-dimensional diffraction data were collected to 1.8 Angstrom at the Laboratorio Nacional de Luz Sincrotron (LNLS, Campinas, Brazil). The crystals belong to the hexagonal system ( space group P6(1) or P6(5)), with unit-cell parameters a = b = 140.6, c = 113.6 Angstrom. A search for heavy-atom derivatives has been initiated and elucidation of the crystal structure is currently in progress.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Educação - FFC

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Pós-graduação em Ciências Sociais - FFC

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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In the 90s, the Brazilian Secretariat of the Special Education Department has expanded the democratization of education and pupils’ access to schooling, by redefi ning the operation of special education services. With this inclusion policy, the education system has been restructured. An important action, conducted during the period de 2005 to 2010, was to propose, deploy and implement Multifunctional Resources Rooms (Sala de recursos multifuncionais, or SRM), from two different types (type I and II). The challenge was to promote educational inclusion, in a program entitled Inclusive Education: the right to diversity. In a seek for new identity of teacher training and professional practice in special education, the great educational innovation is to better articulate the work of “ordinary“ and specialized teachers, via the SRM, and to develop collective and participatory actions between common spaces and support spaces. In this context, the school and the family represent an important source of partnership.

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The energy crisis has affected many countries. With the growing warning with the emission in the atmosphere and the lack of resources, the seek for sustainable sources for energy genaration have become even bigger. Some Countries, as Germany, started first in this journey, creating an incentive program to self-generation with renewable sources (wind, photovoltaics, biomass, etc.), giving priority for smaller plants. In Germany the program called EEG started in 2004. In Brazil, since the beggining of 2012, the self-generators did not know how they could be beneficted for self-generation, and self-generation didn't become commun in the country. However, with NR 482, of April 17th, 2012, the parameters were defined, and the self-generator could have a guideline. Therewith, studyies can be redirected for a better knowlegde of the conditions the self-generator will be sujected, in addition to Germany's case as reference to compare with Brazil's case. In this paper these studies are made, focused in wind power (wind turbines) and photovoltaic panels