Systematics of spiny-backed treefrogs (Hylidae: Osteocephalus): An Amazonian puzzle

Spiny-backed tree frogs of the genus Osteocephalus are conspicuous components of the tropical wet forests of the Amazon and the Guiana Shield. Here, we revise the phylogenetic relationships of Osteocephalus and its sister group Tepuihyla, using up to 6134 bp of DNA sequences of nine mitochondrial and one nuclear gene for 338 specimens from eight countries and 218 localities, representing 89% of the 28 currently recognized nominal species. Our phylogenetic analyses reveal (i) the paraphyly of Osteocephalus with respect to Tepuihyla, (ii) the placement of 'Hyla' warreni as sister to Tepuihyla, (iii) the non-monophyly of several currently recognized species within Osteocephalus and (iv) the presence of low (<1%) and overlapping genetic distances among phenotypically well-characterized nominal species (e.g. O. taurinus and O. oophagus) for the 16S gene fragment used in amphibian DNA barcoding. We propose a new taxonomy, securing the monophyly of Osteocephalus and Tepuihyla by rearranging and redefining the content of both genera and also erect a new genus for the sister group of Osteocephalus. The colouration of newly metamorphosed individuals is proposed as a morphological synapomorphy for Osteocephalus. We recognize and define five monophyletic species groups within Osteocephalus, synonymize three species of Osteocephalus (O. germani, O. phasmatus and O. vilmae) and three species of Tepuihyla (T. celsae, T. galani and T. talbergae) and reallocate three species (Hyla helenae to Osteocephalus, O. exophthalmus to Tepuihyla and O. pearsoni to Dryaderces gen. n.). Furthermore, we flag nine putative new species (an increase to 138% of the current diversity). We conclude that species numbers are largely underestimated, with most hidden diversity centred on widespread and polymorphic nominal species. The evolutionary origin of breeding strategies within Osteocephalus is discussed in the light of this new phylogenetic hypothesis, and a novel type of amplexus (gular amplexus) is described. © 2013 The Norwegian Academy of Science and Letters.

Lâminas de irrigação e cobertura do solo na produção e qualidade de frutos da gravioleira

The crop of soursop has been known for presenting great potential for the domestic market and good prospects for export. In this context, an experiment was conducted in the municipality of Remigio in the state of Paraiba, in order to evaluate the effects of irrigation depths applied weekly on the productive behavior and postharvest quality of fruits of soursop 'Morada' in the soil with and without mulching with crop residues. Treatments were arranged in randomized blocks with three replications and three plants per plot, using a factorial arrangement 5×2, referring to the soil with and without mulching with crop residues, and five irrigation depths of 0, 4.3, 8.6, 12.9 and 17.2 mm plant1. Irrigation was performed manually, once a week from September 2008 to March 2009. The presence of mulch on the soil and increasing depths of irrigation elevated the values of mean mass, production per plant and yield of soursop 'Morada', and decreased the levels of soluble solids.

High Levels of Diversity Uncovered in a Widespread Nominal Taxon: Continental Phylogeography of the Neotropical Tree Frog Dendropsophus minutus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utilização do método AHP para análise de alterações de projetos, em instalações hospitalares

Customer focus , high competitiveness and striving for excellence in quality of service are some of the main features of the current market . Planned and make decisions with greater certainty is no longer an option a few years . The current managers are seeking ways to eliminate waste and increase financial and productive profitability of your company in order to achieve higher performance at a lower cost . The AHP is one of the main concepts of this goal by providing an analysis on multiple criteria , enhancing decision-making and enhancing the gains , with a technique that is designed to take into account all the key criteria for choosing an alternative , according to the perspective of the designer as both suppliers and customers . Emerging as a new way to minimize errors , and make an informed and consistent decision. The use of this method in construction projects is a recent phenomenon , which has much to be explored , an intense analysis of the processes is required to enable it to consider the concepts and present a consistent and grounded proposal