The QUEST for Brazilian space research

This paper describes how QUEST 1 (QUaternion ESTimator algorithm) influenced Brazilian space research activities. Indeed, we present a short survey paper on researches in attitude determination and propagation in Brazil arising from the influence of the author of QUEST. We show how Brazilian researchers started implementing QUEST, tasting it, and later deriving other applications based on it. Some Brazilian researchers worked out further investigations through direct interaction with the QUEST author, Dr. Malcolm Shuster, addressing attitude alignment and calibration problems. Further related researches show the influence of Dr. Shuster's work on Brazilian space research.

The quest for an intermediate-scale accidental axion and further ALPs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effects of inter-crop cultivation Between rows of citrus crop on spreading of Guignardia citricarpa Ascospores and in the citrus black spot occurrence

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da tricostatina A sobre a acetilação de histonas, proliferação celular e diferenciação de células tronco embrionárias murinas

Background: Embryonic stem cells are cells derived from early-stage embryos that are characterized by pluripotency and self-renewal capacity. The in vitro cultured murine embryonic stem cells can indefinitely propagate in an undifferentiated state in the presence of leukemia inhibitory factor (LIF). However, when stimulated, these cells can differentiate into cell lines derived from all three embryonic germ layers. The trichostatin A (TSA) is an epigenetic modifier agent and several studies have used the TSA to stimulate cellular differentiation. However, most of these studies only assessed one TSA concentration. Therefore, this study aimed to evaluate the effects of different TSA concentrations on histone hyperacetylation during in vitro cell differentiation of murine pluripotent embryonic stem cells, cultured with or without LIF, in the quest of to standardize their application on early cultures of embryonic stem cells.Materials, Methods & Results: Undifferentiated murine embryonic stem cells were plated in the presence of different TSA concentrations (0 nM, 15 nm, 50 nM and 100 nM) in the presence or absence of LIF. Thus, the treatments were evaluated in undifferentiated embryonic stem cells cultured in the presence of LIF (Control group: 0 nM LIF(+); Group 15 nM LIF+; Group 50 nM LIF+ and Group 100 nM LIF+), and in embryonic stem cells cultured in the absence of LIF (Control group: 0 nM LIF; Group 15 nM LIF(-); Group 50 nM LIF(-) and Group 100 nM LIF-). Treatment with TSA was performed for 24 h. After that the medium was replaced with fresh medium without TSA. Samples were collected at 0, 12, 24, 36 and 48 h after the beginning of the experiment. Three replicates were performed in each experimental group. The relative amount of Histone H3 lysine 9 acetylation was analyzed in all groups, as well as the cell proliferation in the embryonic stem cells cultured in the presence of LIF. In the control group (0 nM), the absence of LIF resulted in higher levels (P < 0.05) of H3lys9ac compared to the cultures supplemented with LIF. In the embryonic stem cells cultured in the presence of LIF, the 50 nM and 100 nM treatments resulted in higher levels (P < 0.05) of H3lys9ac when compared with 0 nM and 15 nM treatments. Evaluating the Hoechst area in the 0 nM group, it was observed that the number of cells increased (P < 0.05) according to the time of culture. Treatment with 15 nM also reflected a similar distribution, but the Hoechst area in 15 nM group was lower (P < 0.05) at 24 and 48h when compared to the observed in the control group. In the 100 nM treatment, was observed that the area of Hoechst was lower (P < 0.05) to that obtained in the control group at 12, 24 and 48h. In addition, it was observed that treatment with TSA induces greater cellular differentiation when compared to control groups in stem cells cultured in the presence of LIF as well as in the absence of LIF.Discussion: In the present study it was observed that TSA treatment increased the levels of histone acetylation in murine embryonic stem cells at a 50 nM concentration, making it possible to reduce the concentration recommended in the literature (100 nM). In addtion, it was concluded that the lower TSA concentrations utilized (15 nm and 50 nM) was less harmful to cellular proliferation than the 100 nM TSA concentration.

Trabalho e atividade: categorias de análise na psicologia histórico-cultural do desenvolvimento

Este artigo é resultado de estudos teóricos e pretende apresentar algumas contribuições ao estudo da psicologia histórico-cultural do desenvolvimento, mais especificamente no que se refere à relação existente entre as categorias de trabalho e atividade. Estudos desta natureza são importantes para psicólogos, pedagogos e demais profissionais envolvidos direta ou indiretamente com a prática educativa, na busca por compreender o problema da periodização do desenvolvimento em uma perspectiva não naturalizante. Nesta direção, a categoria de atividade principal mostra-se central como força motriz do processo de desenvolvimento humano, portanto encontra-se estreitamente ligada ao lugar que cada indivíduo ocupa na sociedade de classes bem como às condições objetivas de sua existência material. O estudo do desenvolvimento coincide com o estudo da pessoa concreta, imersa numa trama de relações sociais e num sistema político e econômico; enfim, outra coisa não é senão o estudo da história objetivada em cada indivíduo particular.

Filmes na formação de futuros professores: educar o olhar

Neste artigo, trago parte de minha experiência como professora de futuros professores de Matemática quando ministrei a disciplina Didática, no ano de 2010. Socializo aqui algumas das práticas instituídas nesse contexto e que resgatam a potencialidade dos filmes e da escrita no processo formativo dos alunos. Problematizo a importância de assistir juntos a um filme, já que essa experiência estética possibilita promover o conhecimento, o sentimento e a ação. Trago a voz dos meus alunos, documentada nos registros semanais elaborados por eles, para dizer das marcas que os filmes deixaram no percurso formativo de cada um como futuros professores de Matemática. Ao resgatar essa prática, enfatizo a importância de educar o olhar na busca pela constituição de um professor comprometido com uma educação e uma sociedade mais justa e democrática.

A RIEMANN INTEGRAL APPROACH TO FEYNMANS PATH-INTEGRAL

It is a well known result that the Feynman's path integral (FPI) approach to quantum mechanics is equivalent to Schrodinger's equation when we use as integration measure the Wiener-Lebesgue measure. This results in little practical applicability due to the great algebraic complexibity involved, and the fact is that almost all applications of (FPI) - ''practical calculations'' - are done using a Riemann measure. In this paper we present an expansion to all orders in time of FPI in a quest for a representation of the latter solely in terms of differentiable trajetories and Riemann measure. We show that this expansion agrees with a similar expansion obtained from Schrodinger's equation only up to first order in a Riemann integral context, although by chance both expansions referred to above agree for the free. particle and harmonic oscillator cases. Our results permit, from the mathematical point of view, to estimate the many errors done in ''practical'' calculations of the FPI appearing in the literature and, from the physical point of view, our results supports the stochastic approach to the problem.

Gene discovery in Boophilus microplus, the cattle tick - the transcriptomes of ovaries, salivary glands, and hemocytes

The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.

Development of a topical formulation containing S. Lutea extract: Stability, in vitro studies and cutaneous permeation

Flavonoids have been widely incorporated into cosmetic and dermatological formulations, affording benefits such as antioxidant action, improved skin tone and fewer lines and wrinkles. Brazil has a huge biodiversity, with one of the richest flora in the world, and existing studies justify the quest for greater research efforts in this area. The cajazeira (Spondias lutea L.), a plant of the Anacardiaceae family from tropical America, is widely disseminated in Brazil. This plant was chosen because of the presence of flavonoids that exhibit antioxidant activity. The purpose of this research was to develop a stable topical formulation containing Spondias lutea extract with the aim of preventing skin diseases caused by UV radiation. Hydro ethanolic extract of Spondias lutea fruit was prepared and assayed for its the flavonoids content. The antioxidant activity was estimated by DPPH and superoxide assay. The physicochemical stability and skin permeation of the cream containing 8% (w/w) of extract were assessed. The results showed that the S. lutea extract possessed antioxidant activity, and that it is possible to obtain a stable cosmetic containing the extract, which is able to penetrate the skin. Thus, it is possible to use this extract to produce an anti-aging cosmetic.

Obtenção de micro/nanopartículas poliméricas para o encapsulamento do fármaco bromidrato de galantamina

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Rastreamento de leveduras autóctonas para a produção de pectinase, tanase e invertase

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

A legibilidade do visível: as imagens de Oliviero Toscani, um processo de ruptura

Pós-graduação em Comunicação - FAAC

Bressonianos: comprovando a influência do fotógrafo francês Henri Cartier-Bresson sobre fotojornalistas brasileiros atuais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A política agrícola no estado contemporâneo: obrigações jurídicas atinentes ao desenvolvimento rural sustentável

Pós-graduação em Direito - FCHS

A guitarra clássico-romântica: história dos métodos de um instrumento

Pós-graduação em Música - IA