Twelve loci from HSA10, HSA11 and HSA20 were comparatively FISH-mapped on river buffalo and sheep chromosomes

Ten type I loci from HSA 10 (IL2RA and VIM), HSA11 (HBB and FSHB) and HSA20 (THBD, AVP/OXT, GNAS1, HCK and TOP1) and two domestic cattle type II loci (CSSM30 and BL42) were FISH mapped to R-banded river buffalo (BBU) and sheep (OAR) chromosomes. IL2RA (HSA 10) maps on BBU 14q13 and OAR13q13, VIM (HSA 10) maps on BBU14q15 and OAR13q15, HBB (HSA11) maps on BBU16q25 and OAR15q23, FSHB (HSA11) maps on BBU16q28 and OAR15q26. THBD (HSA20) maps on BBU 14q15 and OAR13q15 while AVP/OXT. GNAS1, HCK, and TOP I (HSA20) as well as CSSM30 and BL42 map on the same large band of BBU 14q22 and OAR13q22. All loci were mapped on the same homologous chromosomes and chromosome bands of the two species, and these results agree with those earlier reported in cattle homologous chromosomes 15 and 13. respectively, confirming the high degree of both banding and physical map similarities among the bovid species. Indirect comparisons between physical maps achieved on bovid chromosomes and those reported on HSA10, HSA11 and HSA20 were performed. Copyright (C) 2001 S. Karger AG, Basel.

Restriction fragment analysis of the ribosomal DNA of Paratelmatobius and Scythrophrys species (Anura, Leptodactylidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B-bubalis) chromosome 20

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atlas ambiental como subsídio aos estudos de sustentabilidade - a bacia do Araçuaí no Vale do Jequitinhonha - MG

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mapeamento de genes da família das defensinas no genoma do búfalo

Pós-graduação em Zootecnia - FCAV

Optimization of the sampling scheme for maps of physical and chemical properties estimated by kriging

The sampling scheme is essential in the investigation of the spatial variability of soil properties in Soil Science studies. The high costs of sampling schemes optimized with additional sampling points for each physical and chemical soil property, prevent their use in precision agriculture. The purpose of this study was to obtain an optimal sampling scheme for physical and chemical property sets and investigate its effect on the quality of soil sampling. Soil was sampled on a 42-ha area, with 206 geo-referenced points arranged in a regular grid spaced 50 m from each other, in a depth range of 0.00-0.20 m. In order to obtain an optimal sampling scheme for every physical and chemical property, a sample grid, a medium-scale variogram and the extended Spatial Simulated Annealing (SSA) method were used to minimize kriging variance. The optimization procedure was validated by constructing maps of relative improvement comparing the sample configuration before and after the process. A greater concentration of recommended points in specific areas (NW-SE direction) was observed, which also reflects a greater estimate variance at these locations. The addition of optimal samples, for specific regions, increased the accuracy up to 2 % for chemical and 1 % for physical properties. The use of a sample grid and medium-scale variogram, as previous information for the conception of additional sampling schemes, was very promising to determine the locations of these additional points for all physical and chemical soil properties, enhancing the accuracy of kriging estimates of the physical-chemical properties.

The definition of potential infiltration areas in Guaratingueta watershed, Paraiba do Sul Basin, Southeastern Brazil: an integrated approach using physical and land-use elements

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Topological signatures in CMB temperature anisotropy maps

We propose an alternative formalism to simulate cosmic microwave background (CMB) temperature maps in Lambda CDM universes with nontrivial spatial topologies. This formalism avoids the need to explicitly compute the eigenmodes of the Laplacian operator in the spatial sections. Instead, the covariance matrix of the coefficients of the spherical harmonic decomposition of the temperature anisotropies is expressed in terms of the elements of the covering group of the space. We obtain a decomposition of the correlation matrix that isolates the topological contribution to the CMB temperature anisotropies out of the simply connected contribution. A further decomposition of the topological signature of the correlation matrix for an arbitrary topology allows us to compute it in terms of correlation matrices corresponding to simpler topologies, for which closed quadrature formulas might be derived. We also use this decomposition to show that CMB temperature maps of (not too large) multiply connected universes must show patterns of alignment, and propose a method to look for these patterns, thus opening the door to the development of new methods for detecting the topology of our Universe even when the injectivity radius of space is slightly larger than the radius of the last scattering surface. We illustrate all these features with the simplest examples, those of flat homogeneous manifolds, i.e., tori, with special attention given to the cylinder, i.e., T-1 topology.

Finding critical exponents for two-dimensional Hamiltonian maps

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fitting hyperbolic universes to Cayon-Smoot spots in COBE maps

On the possibility that the universe's matter density is low (Ohm(0) < 1), cosmologies can be considered with the metric of Friedmann's open universe but with closed hyperbolic manifolds as the physical three-space. These models have nontrivial spatial topology, with the property of producing multiple images of cosmic sources. Here a fit is attempted of 10 of these models to the physical cold and hot spots found by Cayon & Smoot in the COBE/DMR maps. These spots are interpreted as early, distant images of much nearer sources of inhomogeneity. The source for one of the cold spots is seen as the seed of a known supercluster.

Developing a research agenda for promoting physical activity in Brazil through environmental and policy change

Objective. To identify the highest priorities for research on environmental and policy changes for promoting physical activity (PA) in Brazil; to uncover any gaps between researchers' and practitioners' priorities; and to consider which tools, methods, collaborative strategies, and actions could be useful to moving a research agenda forward.Methods. This was a mixed-methods study (qualitative and quantitative) conducted by Project GUIA (Guide for Useful Interventions for Activity in Brazil and Latin America) in February 2010-January 2011. A total of 240 individuals in the PA field (186 practitioners and 54 researchers) were asked to generate research ideas; 82 participants provided 266 original statements from which 52 topics emerged. Participants rated topics by "importance" and "feasibility;" a separate convenience sample of 21 individuals categorized them. Cluster analysis and multidimensional scaling were used to create concept maps and pattern matches.Results. Five distinct clusters emerged from the concept mapping, of which " effectiveness and innovation in PA interventions" was rated most important by both practitioners and researchers. Pattern matching showed a divergence between the groups, especially regarding feasibility, where there was no consensus.Conclusions. The study results provided the basis for a research agenda to advance the understanding of environmental and policy influences on PA promotion in Brazil and Latin America. These results should stimulate future research and, ultimately, contribute to the evidence-base of successful PA strategies in Latin America.

Spatial variability of infiltration and its relationship to some physical properties

Water infiltration into soil is one of the basic factors for estimating irrigation intensity according to the plants' requirements; this is aimed at avoiding problems of surface run-off and degradation. The purpose of the present investigation was to determine the spatial variation of infiltration and its relationship to some physical properties of soil by means of geostatistical techniques in Typic Plinthaquult soils having average texture and flat relief. A 113 point mesh was designned, having a regular distance of 10 m between points, samples being taken from 0 to 0.20 meters depth. Sand, silt and clay content, bulk density, macroporosity, microporosity and total porosity were determined. Infiltration tests were carried out in the field by means of a 15 cm diameter ring. Descriptive statistics and geostatistics were used for analysing the data. Infiltration, silt and microporosity data did not fit a normal distribution curve. Infiltration had high variability, having an average 36.03 mm h(-1). Total porosity was 56.73%, this being the only property that did not show spatial dependency. The smallest ranges were observed for bulk density, macroporosity and microporosity, having values of less than 40 m. The smallest degrees of spatial dependence were observed for infiltration, silt and clay, evidence also being shown of the influence of silt and clay on infiltration rate. Contour maps were constructed; fitting them to the semivariogram models, together with studying the correlations, led to establishing relationships between the properties.