Altered Glucose Homeostasis and Hepatic Function in Obese Mice Deficient for Both Kinin Receptor Genes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of different intensities of physical exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice

Objetivo: Investigar os efeitos do exercício físico agudo com diferentes intensidades sobre a sensibilidade à insulina e a atividade da proteína quinase B/Akt no músculo esquelético de camundongos obesos. Método: Foram utilizados camundongos Swiss, divididos aleatoriamente em quatro grupos, que receberam dieta padrão (grupo controle) ou dieta hiperlipídica (grupos obeso sedentário e grupos obesos exercitados 1 e 2), por período de 12 semanas. Dois diferentes protocolos de exercício foram utilizados: natação durante 1 hora com ou sem sobrecarga de 5% da massa corporal. O teste de tolerância à insulina foi realizado para estimar a sensibilidade à insulina. E os níveis protéicos da proteína quinase B/Akt e de sua fosforilação foram determinados no músculo esquelético dos camundongos, através da técnica de Western blot. Resultado: Uma sessão de exercício físico foi capaz de inibir a resistência à insulina em decorrência de uma dieta hiperlipídica. Foi possível demonstrar um aumento na fosforilação da proteína quinase B/Akt, melhora da sinalização da insulina e redução da glicemia de jejum nos camundongos que realizaram 1 hora de natação sem sobrecarga adicional e nos camundongos que realizaram 1 hora de natação com sobrecarga adicional de 5% de sua massa corporal. Entretanto, não houve diferença significativa entre os grupos que realizaram o exercício em diferentes intensidades. Conclusão: Independente da intensidade, o exercício físico aeróbio conseguiu aumentar a sensibilidade à insulina e a fosforilação da proteína quinase B/Akt, revelando ser uma boa forma de tratamento e prevenção do diabetes tipo 2.

Exercise training decreases mitogen-activated protein kinase phosphatase-3 expression and suppresses hepatic gluconeogenesis in obese mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Preventive Effects of Chitosan Coacervate Whey Protein on Body Composition and Immunometabolic Aspect in Obese Mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Macadamia Oil Supplementation Attenuates Inflammation and Adipocyte Hypertrophy in Obese Mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Moderate Exercise Reduces Food Consumption in Obese Mice for Activate Jak-2/Stat-3 Pathway in the Hypothalami

It is very known that due to inflammatory processes the obesity leads to resistance to leptin, it reduces phosphorylation via JAK-2/STAT-3, which generates lower STAT-3 activity in the cell nucleus, and it leads to decrease the number of transcription of anorexigenic neurons (POMC/CART) and allowing transcription of orexigenic (NPY/AgRP). PURPOSE: The present study aimed to evaluate the effects of moderate aerobic training on food intake of obese mice through analysis of activity of hypothalamic proteins JAK-2/STAT-3. METHODS: It were used 30 Swiss mice (30 days old) divided into 3 groups: Control Group (C): sedentary animals fed with balanced diet ; Obese (OB) sedentary animals fed with high-fat diet throughout the experiment and Trained Obese (TOB) : animals fed with high fat diet throughout the experiment , kept sedentary during the first half of the experiment (8 weeks) and submitted to physical training protocol during the second half of the experiment (8 weeks). The exercise program consisted of treadmill running 1h, 5 days/week during 8 weeks at a speed equivalent to 60 % of maximum potency determined at the beginning of training period. To assess the leptin resistance, after rats were deprived of food for 6h with free access to water, they received i.p injection with leptin (2.0µl, 10-6M), after this, the chow was returned and food intake was determined by measuring the quantity and Kcal consumed at the end of 2h. The hypothalami was removed for determination of JAK-2 and STAt-3 activity. RESULTS: Our results showed that moderate physical exercise was effective in improving the JAK/STAT signaling pathway in the hypothalamus of obese animals. This has made these obese animals had reduced food intake and consequently lower body mass gain. CONCLUSION: It can be concluded that physical exercise, for restoring leptin signaling in the hypothalamus, controls the synthesis of neurons responsible for appetite and thus is an important tool in the treatment of obesity.

Oxidative stress and inflammatory mediators contribute to endothelial dysfunction in high-fat diet-induced obesity in mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Role of PKC and CaV1.2 in Detrusor Overactivity in a Model of Obesity Associated with Insulin Resistance in Mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Corrective effects of acerola (Malpighia emarginata DC.) juice intake on biochemical and genotoxical parameters in mice fed on a high-fat diet

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Obesity enhances eosinophilic inflammation in a murine model of allergic asthma

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação preliminar dos efeitos de Brassica campestris sobre a obesidade induzida por dieta hipercalórica

Obesity along with overweight has been considered one of the most serious public health problems in the world, especially because they are the main risk factors for many chronic diseases such as coronary heart disease, type II diabetes mellitus, hypertension and some types of tumors, which are associated with high mortality rates. The use of functional foods and appropriate diets to promote health has grown as a mechanism for prevention, control and treatment of chronic diseases such as obesity. Several ethnopharmacological studies indicate plants species for the treatment of disorders associated with obesity with a major attraction of this regimen is perceived as safer and more effective for health than the traditional treatment with appetite suppressants. Whereas both food intake and the oral treatment with different compounds can cause changes in gene expression and that a proper diet has been valuable as a mechanism for maintaining the body's vital functions, the objective of this project was to evaluate the effects of standardized extract of Brassica campestris L. in the decrease in weight and food intake control. In vivo trials of this product were conducted and studies of its effects on energy metabolism in non-obese mice and with obesity induced by hypercaloric diet. After induction of obesity by 8 weeks, animals were treated for 21 days with the extract orally. After 21 days the animals were killed and the effects of this product were evaluated on the daily feed intake and on body weight. According to the results obtained, the extract of Brassica campestris was not effective in reducing body weight of obese animals, and did not reduce food intake

Avaliação preliminar dos efeitos de Coffea arabica sobre a obesidade induzida por dieta hipercalórica

Obesity along with overweight, has been considered one of the most serious public health problems in the world, especially because they are the main risk factors for many chronic diseases, such as coronary heart disease, type II diabetes mellitus, hypertension and some types of tumors, which are associated with high mortality rates. The use of functional foods and appropriate diets to call health promotion has grown as a mechanism for prevention, control and treatment of chronic diseases such as obesity. Several ethnopharmacological studies indicate plant species for the treatment of disorders associated with obesity with a major attraction of this regimen is perceived as safer and more effective health than the traditional treatment with appetite suppressants. This work aims to develop and standardize models of biological assays in stress and obesity, also aims to evaluate the effect of oil green beans of Coffea arabica in the regulation of body weight and energy balance in mice. To this end, trials were made in vivo studies of this product and their effects on energy metabolism in non-obese mice with obesity induced by hypercaloric diet. After induction of obesity by 8 weeks, animals were treated for 21 days with the extracts orally. After 21 days the animals were killed to evaluate the effects of these products on daily feed intake and on body weight. The group treated with the oil of Coffea arabica L. showed significant weight loss and feed intake high. According to the results, we conclude that the standardized extract of Coffea arabica L. decreased body weight without restriction or decrease the amount of food ingested

Efeitos do treinamento físico aeróbio sobre o processo inflamatório e apoptótico em neurônios hipotalâmicos de controle da fome em camundongos obesos induzidos por dieta hiperlipídica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)