ATIVIDADE DE PEROXIDASES (EC 1.11.1.7) E TEOR DE PROLINA em FEIJOEIRO Phaseolus vulgaris L. CULTIVADO em CONDIÇÕES DE SALINIDADE

Alterações nos níveis de prolina e na atividade da peroxidase foram estudadas em folhas e raízes de feijoeiro em função da resposta à salinidade do meio. A atividade da peroxidase (E.C. 1.11.1.7) e o teor de prolina das folhas foi maior do que nas raízes. Os resultados mostraram que a atividade da peroxidase diminuiu, enquanto os teores de prolina aumentaram gradualmente nas plantas cultivadas em meio salino. Ocorreu diminuição do teor de potássio e aumento de sódio nos órgãos analisados, com o aumento da concentração salina.

ATIVIDADE DE PEROXIDASES (EC 1.11.1.7) E TEOR DE PROLINA NO EMBRIÃO E COTILÉDONES DE FEIJOEIRO Phaseolus vulgaris L. em CONDIÇÕES DE SALINIDADE

Os efeitos da salinidade sobre a atividade de peroxidases foram acompanhadas no embrião e em cotilédones de feijoeiro Phaseolus vulgaris L. cv. Carioca, observando-se em ambos, aumento na atividade das enzimas. Com relação ao teor de prolina, ocorreu decréscimo constante no embrião de feijoeiro cultivado em condições salinas, entretanto, nos cotilédones, verificou-se aumento progressivo em condições de presença ou ausência de salinidade.

SISTEMAS QUIMILUMINESCENTES COM PEROXIDASE (EC:1.11.1.7) E SUAS APLICACOES EM ANALISES CLINICAS

The enzyme horseradish peroxidase HRP (EC:1.11.1.7), has both acid and basic isoenzymes, catalyses a wide range of reactions (acting as an oxiredutase or an oxidase) and is thought capable of one- or two-electrons oxidations depending on the substrate employed. Today, the methodology for these assay can be chemiluminescent reactions and enhanced chemiluminescent. The enhanced chemiluminescent assay with system HRP, luminol, peroxide and an enhancer has provided the basis for a convenient and sensitive assay for peroxidase and peroxidase conjugates, DNA probe and blotting assay. It is particularly more advantageous than the others, because is very rapid, more sensitive (attomoles), easy to do and technically simple, and is relatively specific for HRP (reduces the effect of the interference).

Peroxidase enzyme (EC.1.11.1.7) activity as an indicator of water stress in sweet pepper plants

The present work was carried out at the Faculdade de Ciências Agronômicas - UNESP, Botucatu, SP. The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. Sweet pepper plants were grown for 230 days after transplanting of seedlings. The experiment was arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated, as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving the plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.

Atividade da enzima conversora de angiotensina e os níveis plasmáticos de peptídeos do sistema renina-angiotensina (Ang I, Ang II e Ang-(1-7)) em mulheres normotensas e hipertensas no climatério

Pós-graduação em Ciências da Motricidade - IBRC

Interação entre a Angiotensina II, Angiotensina-(1-7) e ECA2 em mulheres

A hipertensão arterial sistêmica é uma doença crônica degenerativa de etiologia multifatorial e poligênica, caracterizada pela presença de níveis tensionais elevados, sendo considerada um dos mais importantes fatores de risco para o desenvolvimento das doenças cardiovasculares. Estudos demonstram que cerca de um bilhão de indivíduos apresentam HAS em todo mundo, sendo esta patologia responsável por 7,1 milhões de morte a cada ano. Diversos fatores estão associados ao aumento da incidência da hipertensão arterial, como obesidade, dislipidemias, dieta rica em sal, alterações nos níveis hormonais e sedentarismo. Após o período da menopausa, observa-se significativa elevação nos valores de pressão arterial dessa população, sendo estes similares ou até maiores aos dos homens para esse mesmo período. O sistema renina-angiotensina (SRAA) desempenha importante papel no controle dos sistemas cardiovascular e renal e a angiotensina II é um potente vasoconstritor desse sistema, formado a partir da ação da Enzima Conversora de Angiotensina (ECA). Por outro lado, a recente descoberta da Enzima Conversora de Angiotensina 2 (ECA2), um homólogo da ECA, mudou todo o conceito do SRAA. A ECA 2 é responsável pela formação do peptídeo angiotensina- (1-7) a partir da clivagem do último aminoácido (fenilalanina) da angiotensina II. Recentes evidências têm demonstrado efeitos benéficos da angiotensina-(1-7) para as doenças cardiovasculares através da vasodilatação, efeito anti-arritmogênico, melhora na função contrátil pós-isquemia e inibição da proliferação celular do músculo liso vascular, sendo esses efeitos antagônicos àqueles observados pela angiotensina II. Dessa forma, o objetivo desse estudo foi analisar a atividade da ECA2, concentrações plasmáticas...(Resumo completo, clicar acesso eletrônico abaixo)

Análises celulares e moleculares dos efeitos do peptídeo vasoativo angiotensina-(1-7) no processo tumoral pulmonar e a atuação do microrna 21-5P

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Endurance training improves responsiveness to insulin and modulates insulin signal transduction through the phosphatidylinositol 3-kinase/Akt-1 pathway

Background: Endurance training increases insulin-stimulated muscle glucose transport and leads to improved metabolic control in diabetic patients.Objective: To analyze the effects of endurance training on the early steps of insulin action in muscle of rats. Design: Male rats submitted to daily swimming for 6 weeks were compared with sedentary controls. At the end of the training period, anesthetized animals received an intravenous (i.v.) injection of insulin and had a fragment of their gastrocnemius muscle excised for the experiments.Methods: Associations between insulin receptor, insulin receptor substrates (IRS)-1 and -2 and phosphatidylinositol 3-kinase (PI3-kinase) were analyzed by immunoprecipitation and immunoblotting. Akt-1 serine phosphorylation and specific protein quantification were detected by immunoblotting of total extracts, and IRS-1/IRS-2-associated PI3-kinase activity were determined by thin-layer chromatography.Results: Insulin-induced phosphorylation of IRS-1 and IRS-2 increased respectively by 1.8-fold (P < 0.05) and 1.5-fold (P < 0.05), whereas their association with PI3-kinase increased by 2.3-fold (P < 0.05) and 1.9-fold (P < 0.05) in trained rats as compared with sedentary controls, respectively. The activity of PI3-kinase associated with IRS-1 and IRS-2 increased by 1.8-fold (P < 0.05) and 1.7-fold (P < 0.05) respectively, in trained rats as compared with their untrained counterparts. Serine phosphorylation of Akt-1/PKB increased 1.7-fold (P < 0.05) in trained rats in response to insulin. These findings were accompanied by increased responsiveness to insulin as demonstrated by a reduced area under the curve for insulin during an i.v. glucose tolerance test, by increased glucose disappearance rate during an insulin tolerance test, and by increased expression of glucose transporter-4.Conclusions: the increased responsiveness to insulin induced by chronic exercise in rat skeletal muscle may result, at least in part, from the modulation of the insulin signaling pathway at different molecular levels.

Hydrodechlorination of 1,2-dichloroethane by rhodium catalysts under water gas shift reaction conditions

Homogeneous catalysts prepared from rhodium trichloride in aqueous aromatic amines have been shown to reduce C-CI bonds under mild water gas shift conditions (T=100 degrees C, P-CO = 1.0 atm). In a 4-picoline/water solvent mixture, 1,2-dichloroethane is reduced to ethylene and ethane in yields compatible with the consumption of the reducing agent CO and with the formation of CO2. Variation of the catalyst solutions by using different substituted pyridines shows a pattern of catalytic activity parallel to that reported previously for H-2 production from the shift reaction, There is a moderate dependence of activity on the basicity of the aromatic amine, but a methyl group at the alpha-position exercises a strong negative steric effect. Long term studies show decrease of the activity with the time perhaps due to the build up of chloride in the medium. (C) 1999 Elsevier B.V. B.V. All rights reserved.

Measurement of the p(p)over-bar -> WZ+X cross section at root s=1.96 TeV and limits on WWZ trilinear gauge couplings

We present measurements of the process p (P) over bar -> WZ + X -> l 'nu(l ')l (l) over bar at root s = 1:96 TeV,where l and l ' are electrons or muons. Using 1 fb(-1) of data from the D0 experiment, we observe 13 candidates with an expected background of 4.5 +/- 0.6 events and measure a cross section sigma(WZ) = 2.7(-1.3)(+1.7) pb. From the number of observed events and the Z boson transverse momentum distribution, we limit the trilinear WWZ gauge couplings to -0: 17 <= lambda(Z) <= 0.21 (Delta k(Z) <= 0.29(lambda(Z) = 0) at the 95% C.L. for a form factor scale Lambda = 2 TeV. Further, assuming that Delta g(1)(Z) = Delta k(Z), we find -0.12 <= Delta k(Z) <= 0.29(lambda(Z) = 0) at the 95% C. L. These are the most restrictive limits on the WWZ couplings available to date.

Properties of l=1 B-1 and B*(2) Mesons

This Letter presents the first strong evidence for the resolution of the excited B mesons B-1 and B-2(*) as two separate states in fully reconstructed decays to B+(*())pi(-). The mass of B-1 is measured to be 5720.6 +/- 2.4 +/- 1.4 MeV/c(2) and the mass difference Delta M between B-2* and B-1 is 26.2 +/- 3.1 +/- 0: 9 MeV/c(2), giving the mass of the B-2* as 5746.8 +/- 2.4 +/- 1.7 MeV/c(2). The production rate for B-1 and B-2* mesons is determined to be a fraction (13.9 +/- 1.9 +/- 3.2)% of the production rate of the B+ meson.

Search for the Higgs boson in H -> WW(*) decays in p(p)over-bar collisions at root(s)=1.96 TeV

We present a search for the standard model Higgs boson in H -> WW(*) decays with e(+)e(-), e(+/-)mu(-/+), and mu(+)mu(-) final states in p (p) over bar collisions at a center-of-mass-energy of root s = 1.96 TeV. The data, collected from April 2002 to june 2004 with the D0 detector, correspond to an integrated luminosity of 300-325 pb(-1), depending on the final state. The number of events observed is consistent with the expectation from backgrounds. Limits from the combination of all three channels on the Higgs boson production cross section times branching ratio sigma x BR(H -> WW(*)) are presented.

Search for Randall-Sundrum Gravitons in the Dielectron and Diphoton Final States with 5.4 fb(-1) of Data from p(p)over-bar Collisions at root s=1.96 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Observation of ZZ Production in p(p)over-bar Collisions at root s=1.96 TeV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Search for CP violation in B-s(0) -> mu(+) D-s(-) X decays in p(p)over-bar collisions at root s=1.96 TeV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)