Molecular and biochemical characterization of the Neurospora crassa glycogen synthase encoded by the gsn cDNA

Glycogen synthases catalyze the transfer of a glucosyl moiety from a nucleotide phosphosugar to a nascent glycogen chain via an alpha1-->4 linkage. Although many genes coding for glycogen synthases have been described, the enzymes from rabbit and yeast are the best characterized. The fungus Neurospora crassa accumulates glycogen during exponential growth, and mobilizes it at the onset of stationary phase, or when placed at high temperature or starved for carbon. Through a PCR methodology, the gsn cDNA coding for the N. crassa glycogen synthase was isolated, and the amino acid sequence of the protein was deduced. The product of the cDNA seems to be the only glycogen synthase present in N. crassa. Characterization of the gsn cDNA revealed that it codes for a 706-amino acids protein, which is very similar to mammalian and yeast glycogen synthases. Gene expression increased during exponential growth, reaching its maximal level at the end of the exponential growth phase, which is consistent with the pattern of glycogen synthase activity and glycogen level. Expression of the gsn is highly regulated at the transcriptional level. Under culture conditions that induce heat shock, conidiation, and carbon starvation, expression of the gsn gene was decreased, and glycogen synthase activity and glycogen content behaved similarly.

Activity of the Ovarian Germinal Epithelium in the Freshwater Catfish, Pimelodus maculatus (Teleostei: Ostariophysi: Siluriformes): Germline Cysts, Follicle Formation and Oocyte Development

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do potencial genotóxico e mutagênico de águas e sedimentos do rio Guaecá, São Sebastião - SP, após impacto de oleoduto da região

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Qualidade da água ao longo do Rio Capivara no município de Botucatu - SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização hídrica em função das condições de uso e manejo do solo na microbacia hidrográfica do córrego da Fazenda da Glória, Taquaritinga, S.P

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Simulação do enovelamento de proteínas desnaturadas utilizando o método de crescimento de cadeias em rede tetraédrica

Pós-graduação em Biofísica Molecular - IBILCE

A endemia da malária em Porto Velho (RO): um estudo baseado na análise estatítica espacial de dados multivariados

Pós-graduação em Geociências e Meio Ambiente - IGCE

Análise de um conjunto de procedimentos metodológicos para a delimitação de corredores verdes (Greenways) ao longo de cursos fluviais

Pós-graduação em Geociências e Meio Ambiente - IGCE

O integralismo pliniano: autoritarismo e ordem na defesa da nação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A crise financeira de 2008: os discursos e as estratégias do governo e dos fundos de pensão

This article focuses on the financial crisis beginning in 2008. Drawing on the work of Lebaron (2010; 2011) and (Grün 2010), the study seeks to grasp the cognitive dimension of the crisis through the discourses produced (and reproduced) by members of the Brazilian government involved in controlling the crisis and by the pension fund sector and its strategies. The method was based on analysis of documents produced by the pension fund sector and the Lula Administration in 2008 and the spinoffs of the discourses and strategies. The text indicates the construction of a discourse emphasizing the importance of state regulation (as opposed to market self-regulation) and the central role of pension funds during the process, since they partially abandoned government bonds and migrated to productive investment, in alliance with the private equity sector, especially in financing construction works under the Growth Acceleration Program.

eIF5A has a function in the cotranslational translocation of proteins into the ER

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prática e consciência das regras no jogo de Bola Queimada: contribuições para a educação física

Pós-graduação em Educação - FFC

Single-cell expression profiling reveals a dynamic state of cardiac precursor cells in the early mouse embryo

In the early vertebrate embryo, cardiac progenitor/precursor cells (CPs) give rise to cardiac structures. Better understanding their biological character is critical to understand the heart development and to apply CPs for the clinical arena. However, our knowledge remains incomplete. With the use of single-cell expression profiling, we have now revealed rapid and dynamic changes in gene expression profiles of the embryonic CPs during the early phase after their segregation from the cardiac mesoderm. Progressively, the nascent mesodermal gene Mesp1 terminated, and Nkx2-5+/Tbx5+ population rapidly replaced the Tbx5low+ population as the expression of the cardiac genes Tbx5 and Nkx2-5 increased. At the Early Headfold stage, Tbx5-expressing CPs gradually showed a unique molecular signature with signs of cardiomyocyte differentiation. Lineage-tracing revealed a developmentally distinct characteristic of this population. They underwent progressive differentiation only towards the cardiomyocyte lineage corresponding to the first heart field rather than being maintained as a progenitor pool. More importantly, Tbx5 likely plays an important role in a transcriptional network to regulate the distinct character of the FHF via a positive feedback loop to activate the robust expression of Tbx5 in CPs. These data expands our knowledge on the behavior of CPs during the early phase of cardiac development, subsequently providing a platform for further study.

Recuperação de área de preservação permanente através da nucleação como subsídio à adequação legal da atividade de extração de argila em pequena propriedade

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)