Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type

The complete nucleotide sequence of the genomic RNA 1 (8745 nt) and RNA 2 (4986 nt) of Citrus leprosis virus cytoplasmic type (CiLV-C) was determined using cloned cDNA. RNA 1 contains two open reading frames (ORFs), which correspond to 286 and 29 kDa proteins. The 286 kDa protein is a polyprotein putatively involved in virus replication, which contains four conserved domains: methyltransferase, protease, helicase and polymerase. RNA 2 contains four ORFs corresponding to 15, 61, 32 and 24 kDa proteins, respectively. The 32 kDa protein is apparently involved in cell-to-cell movement of the virus, but none of the other putative proteins exhibit any conserved domain. The 5' regions of the two genomic RNAs contain a 'cap' structure and poly(A) tails were identified in the 3'-terminals. Sequence analyses and searches for structural and non-structural protein similarities revealed conserved domains with members of the genera Furovirus, Bromovirus, Tobravirus and Tobamovirus, although phylogenetic analyses strongly suggest that CiLV-C is a member of a distinct, novel virus genus and family, and definitely demonstrate that it does not belong to the family Rhabdoviridae, as previously proposed. Based on these results it was proposed that Citrus leprosis virus be considered as the type member of a new genus of viruses, Cilevirus.

Design of novel iron compounds as potential therapeutic agents against tuberculosis

In the search for new therapeutic tools against tuberculosis two novel iron complexes, [Fe(L-H)3], with 3-aminoquinoxaline-2-carbonitrile N(1),N(4)-dioxide derivatives (L) as ligands, were synthesized, characterized by a combination of techniques, and in vitro evaluated. Results were compared with those previously reported for two analogous iron complexes of other ligands of the same family of quinoxaline derivatives. In addition, the complexes were studied by cyclic voltammetry and EPR spectroscopy. Cyclic voltammograms of the iron compounds showed several cathodic processes which were attributed to the reduction of the metal center (Fe(III)/Fe(II)) and the coordinated ligand. EPR signals were characteristic of magnetically isolated high-spin Fe(III) in a rhombic environment and arise from transitions between m(s) = +/- 1/2 (geff-9) or m(s) = +/- 3/2 (g(eff)similar to 4.3) states. Mossbauer experiments showed hyperfine parameters that are typical of high-spin Fe(III) ions in a not too distorted environment. The novel complexes showed in vitro growth inhibitory activity on Mycobacterium tuberculosis H(37)Rv (ATCC 27294), together with very low unspecific cytotoxicity on eukaryotic cells (cultured murine cell line J774). Both complexes showed higher inhibitory effects on M. tuberculosis than the "second-line" therapeutic drugs. (C) 2010 Elsevier B.V. All rights reserved.

The GHEP-EMPOP collaboration on mtDNA population data-A new resource for forensic casework

Mitochondrial DNA (mtDNA) population data for forensic purposes are still scarce for some populations, which may limit the evaluation of forensic evidence especially when the rarity of a haplotype needs to be determined in a database search. In order to improve the collection of mtDNA lineages from the Iberian and South American subcontinents, we here report the results of a collaborative study involving nine laboratories from the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) and EMPOP. The individual laboratories contributed population data that were generated throughout the past 10 years, but in the majority of cases have not been made available to the scientific community. A total of 1019 haplotypes from Iberia (Basque Country, 2 general Spanish populations, 2 North and 1 Central Portugal populations), and Latin America (3 populations from São Paulo) were collected, reviewed and harmonized according to defined EMPOP criteria. The majority of data ambiguities that were found during the reviewing process (41 in total) were transcription errors confirming that the documentation process is still the most error-prone stage in reporting mtDNA population data, especially when performed manually. This GHEP-EMPOP collaboration has significantly improved the quality of the individual mtDNA datasets and adds mtDNA population data as valuable resource to the EMPOP database (www.empop.org). (C) 2010 Elsevier B.V. All rights reserved.

Research of new mixed-chelate copper complexes with quinoxaline N 1,N 4,-dioxide derivatives and alanine as ligands, potential antimycobacterial agents

Three new mixed-chelate copper complexes with 3-aminoquinoxaline-2-carbonitrile N 1,N 4-dioxide derivatives and alanine as ligands were synthesized in solid state. The spectroscopic characterization (FTIR, EPR, UV-Vis) showed that copper coordinated through the amine and the N-oxide groups of the quinoxaline derivatives and the amine and carboxylate moieties from alanine forming a dimeric species. The tree complexes showed in vitro activity against M. tuberculosis H 37Rv (ATCC 27294) similar to that of ethambutol while they are inactive against E. coli and S. aureus.

Mangá: uma nova gênese : análise da história em quadrinhos Neon Genesis Evangelion

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Discussões sobre políticas para mulher e família na Rússia revolucionária

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mito, lenda e história em Matrinchã do Teles Pires: a construção do terreno ficcional

Pós-graduação em Letras - IBILCE

Efeitos da Fisioterapia Respiratória na Mecânica Respiratória de Pacientes com Acidente Vascular Encefálico Ventilados Mecanicamente

Patients who went through a Stroke may require mechanical ventilation (MV) in the acute phase of the disease and, on MV, they may show alterations in the respiratory mechanic. Physiotherapy techniques are applied in patients on MV to improve their respiratory mechanic. Thus, the purpose of this study was to evaluate the effects of the Respiratory Physiotherapy into the dynamic compliance (Cdyn), static compliance (Cst) and airway resistance (Rwa) in Stroke patients on MV. In order to do that, patients with Stroke diagnostic on MV were evaluated, before and after the use of Physiotherapy techniques (manual rib-cage compression, thoracic decompression, zero end expiratory pressure and suctioning). Cdyn, Cst and Rwa were the researched variables. Statistical Analysis was made using Paired T-Test with statistical significance with p-values no greater than 5%. Eleven patients were part of the study, with an average of 64,6±12,5 years old. Cdyn increased after physiotherapy from 29,3±16,1 ml/cmH2O to 33,8±16,7 ml/cmH2O (p=0,03). Cst also increased from 44,4±20,7 ml/cmH2O to 54,0±26,6 ml/cmH2O (p=0,024). No significant difference was detected for Rwa between the before and after moments (Before – 8,0±3,2 cmH2O/l/s, After – 7,3±2,25 cmH2O/l/s; p=0,45). It was possible to conclude that both dynamic and static compliance increased after physiotherapy, but the airway resistance did not increase in patients with stroke on mechanical ventilation.

Ações judiciais de Plínio, o jovem, no Tribunal dos Centúnviros e na Corte Senatorial (séculos I-II d.C.)

Pós-graduação em História - FCHS

Retratos da Espanha em Os fantasmas de Goya

1792, Madri. After years of inactivity, the Spanish Inquisition is born again with the mission of containing the laic winds that blow from the revolutionary France. Inês Bilbatúa, a rich merchant’s daugther, is victim of the inquisitorial machinery which tortures and violates her, through one of their abetters, the Dominican monk Lorenzo. Before being arrested, the young lady had served as model for the painter Francisco of Goya, who had also portrayed the monk Lorenzo. The Aragonese painter’s figure serves as narrative conductor of a history that narrates the young Inês’ via crucis and, at the same time, it recreates the historical scenery of the Napoleonic invasion (1808), through a basic element, the painting. Our work intends to analyze the relationships among movie, painting and history present in “Goya’s ghosts” (2006), of the Czech director Milos Forman (1932) - whose script was adapted to a homonymous book in 2007 -, a movie that is based on the artistic production of Francisco of Goya y Lucientes (1746 -1828), official painter of Carlos’ IV (1788 -1808) court and the most lucid columnist of his time, that knew how to capture in his works the religious fanaticism, the populist fervour, the governor’s hypocrisy and the horror and the violence of the war.

Aquisição da informação visual: controle da postura e do olhar

Postural control is needed to perform various daily activities, from staying in one posture, standing, to sports situations. Many studies have shown that sensory systems help to maintain posture stable; acquisition of perceptual information, particularly through head and eye movements, allows static and dynamic equilibrium. Research related to both motor behavior and physical activities such as gymnastics have contributed to a better understanding of complexities involved in human postural control. The purpose of this study was to review the related literature, searching for possible answers on how everyday and sports actions are performed, with respect to the control and maintenance of posture. Its focus was on how the human body acquires information from the sensory systems, vision in special, and how this information acts to support the control of posture and gaze