Morte e delírio em Théophile Gautier e Villiers de L'Isle-Adam

Pós-graduação em Estudos Literários - FCLAR

A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus

The effects of mildly acidic conditions on the free energy of unfolding (Delta G(u)(buff)) of the pore-forming alpha-hemolysin (alpha HL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography during urea denaturation, Decreasing the pH from 7.0 to 5.0 reduced the calculated Delta G(u)(buff) from 8.9 to 4.2 kcal moI(-1), which correlates with an increased rate of pore formation previously observed over the same pH range, It is proposed that the lowered surface pH of biological membranes reduces the stability of alpha HL thereby modulating the rate of pore formation. (C) 1999 Federation of European Biochemical Societies.

A apostasia comunista: a subjetividade como política

Edgard Morin (Auto-critique), Annie Kriegel (Ce que j'ai cru comprendre) e Claude Roy (Moi, je) percorrem passo a passo, desde a infância, as trilhas que o levaram ao PCF; os três participaram da resistência anti-nazista ao lado da União Soviética, ocasião da entrada em comunismo. Seguem-se as descrições de uma lenta asfixia moral em que os depoimentos fazem perceber como a rede das necessidades internas à subjetividade. Este artigo, analisando as autobiografias de três intelectuais franceses no momento em que dão criticamente conta de seu passado comunista procura apreender a figura histórica toda negativa da relação a si nas assim chamadas contra-sociedades comunistas durante o período stalinista.

A leitura do fantástico nos contos Ligeia de Edgard Allan Poe, e Véra, de Villiers de L'Isle-Adam

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de Lecanicillium lecanii em meios líquidos, sólidos e combinados em sistema bifásico de cultivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caos e cosmo na floresta: o outro e o mal na Amazônia de Darcy Ribeiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A representação feminina na obra de Virginia Woolf: um diálogo entre o projeto político e o estético

Pós-graduação em Estudos Literários - FCLAR

A obra do poeta politradutor Armand Robin: marcas de uma existência em fuga

Armand Robin was a prodigious translator, that is to say: his works were translated into 22 different languages, not to mention his missing texts. During his bulletin on the radio, it was also possible to notice that 18 idioms were fluently received. This poet was notable not only because of his capacity of knowing several languages, but also because of his conception about translation. According to Robin, the meaning was not enough; a target text (TT) must be based on the recreation of the source text (ST), signification by signification; sound by sound, language by language. Since his first book, Ma Vie sans Moi, was released, Robin always wanted his translated texts were presented as works of his own, creating the idea that there was no difference between ST and TT, before giving priority to translation, either in publications or in radio transmissions. In 1942, ÉditionsGallimard published his single “novel”, Le temps qu’ilfait, which was object of studies related to the poet as a translator, emphasizing the pulse of life, which was visible in his TTs. However, in our paper, we are going to observe his production as an author, more specifically his writings, his novel and some of his fragments, in order to deepen the knowledge about his experience and also understand the dramatic and peculiar speech which is present in his writings.

A memória do mundo e a confiança em Yves Bonnefoy

Cette communication prétend parcourir quelques poèmes de Les planches courbes (2001) d’Yves Bonnefoy à partir de l’opposition entre mémoire et oubli. Cette réflexion se trouve esquissée dans l’essai “Sous l’horizon du langage” du livre homonyme, lorsqu’il affirme que la poésie est “ce qui, dans l’espace des mots, notre monde, a mémoire du surcroît de ce qui est sur ses représentations” (2002 : 8). Il s’agit d’une mémoire établie moins à partir d’un lieu perdu que retrouvé. Ce à elle que le moi se confie: dans le “oui”, dans la paix, ces deux mots qui apparaissent dans ses livres les plus récents. Le poète apporterait une confiance qui reprendrait, d’une certaine façon, ce que Paul Ricoeur a caractérisé comme le sens des inscriptions-affections: “elles seraient le dépositaire de la signification la plus dissimulée, mais la plus originaire du verbe “demeurer”, synonyme de “durer”. Dans Les planches courbes, il s’agit également d’une mémoire de l’enfance et des mythes. Ce sont des événements qui prennent place dans un temps primordial. Espoir/confiance, donc, de remonter à une force plus ancienne de la langue, de soi-même, de l’être, de ses rapports. Remonter à une unité dans le simple, en reliant une appréhension du monde et de soi-même dans un acte de suffisance, dans lequel celui-ci se conjugue avec une espèce de dépossession: de l’idée, du concept, de l’image.

A travessia do interlocutor de Cecília Meireles em: Viagem, Vaga música, Mar absoluto e outros poemas e Retrato natural

Pós-graduação em Letras - FCLAS

Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

Objective To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. Results A multilevel factorial design was used to quantify effects of temperature (33–37 C), fresh medium addition after the viral adsorption step (100–200 % with respect to the initial cell suspension volume before infection) and harvest time (8–40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml-1 ) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml-1 ). Conclusion These results establish the basis for designing bioprocess to produce RVGP.

Jules Laforgue e Carlos Drummond de Andrade: a ironia e a construção do gauche

Pós-graduação em Estudos Literários - FCLAR

Sentimento de culpa na obra freudiana: universal e inconsciente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ambiente térmico y el bienestar de los cerdos en el período de descanso previo al sacrificio

The research aimed to evaluate the relationship between the thermal environment of the rest pens in a pig slaughterhouse and the surface temperature (ST) of the animals as well as the effect of intermittent water spraying on thermal comfort. There were two visits to slaughter industrial being in each selected three pens of rest, one for control treatment (no spray throughout the period) and two for the treatment of intermittent spraying water (30 minutes intervals with constant spray followed by 30 minutes then turned off). Assessments began at the time of discharging animals in which are taken the thermographic images from lots using an infrared thermographic camera. After housing in the pens, were registered the thermographic images and the respiratory frequency (RF) every five minutes for four hours. Were evaluated the temperature, relative humidity and temperature-humidity index (THI). Considering the values of THI was observed that the animals remained in a condition of comfort only at the time of his arrival at the slaughterhouse. The ST and RF of the animals kept in the pens without sprinkler system (control) showed a linear increase in function of time of day, with increasing temperature. The treatment with intermittent sprinkling of water was observed oscillatory behavior of the ST and RF, however it was always lower than the control treatment, even in times when the water sprinklers were turned off. The time required of spray to reduction and stabilization of the ST of the animals was limited to the combination of temperature and relative humidity, being estimated at 25 minutes at times of higher THI. The maximum environmental temperature during evaluation was 28.4 oC.