Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus

The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most frequent (35.15%) followed by the trimeric CCG/CGG (12.81%). From a random sample of 1,217 sequences, 343 microsatellites in 265 SSR-containing sequences were identified. Approximately 48% of these ESTs containing microsatellites were homologous to proteins with known biological function. Most of the microsatellites detected in Eucalyptus ESTs were positioned at either the 5 or 3 end. Our next priority involves the design of flanking primers for codominant SSR loci, which could lead to the development of a set of microsatellite-based markers suitable for marker-assisted Eucalyptus breeding programs.

Aquisição da coda vibrante: o estabelecimento de pistas fonético-acústicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Digital monitoring of broiler breeder behavior for assessment of thermal welfare

The assessment of welfare issues has been a challenge for poultry producers, and lately welfare standards needs to be reached in order to agree with international market demand. This research proposes the use of continuous behavior monitoring in order to contribute for assessing welfare. A software was developed using the language Clarium. The software managed the recording of data as well as the data searching in the database Firebird. Both software and the observational methodology were tested in a trial conducted inside an environmental chamber, using three genetics of broiler breeders. Behavioral pattern was recorded and correlated to ambient thermal and aerial variation. Monitoring video cameras were placed on the roof facing the used for registering the bird's behavior. From video camera images were recorded during the total period when the ambient was bright, and for analyzing the video images a sample of 15min observation in the morning and 15 min in the afternoon was used, adding up to 30 min daily observation. A specific model so-called behavior was developed inside the software for counting specific behavior and its frequency of occurrence, as well as its duration. Electronic identification was recorded for 24h period. Behavioral video recording images was related to the data recorded using electronic identification.. Statistical analysis of data allowed to identify behavioral differences related to the change in thermal environment, and ultimately indicating thermal stress and departure from welfare conditions.

Comparação entre métodos de estimativa da evapotranspiração de referência (ETo) em regiões citrícolas paulistas

As regiões escolhidas para o estudo são importantes no cenário citrícola irrigado, tanto em expansão quanto em atuação. Os dados meteorológicos utilizados para as estimativas são integrantes do banco de dados climáticos do software IRRIPLUS®. Com este software, estimou-se a evapotranspiração de referência (ETo) diária, utilizando as metodologias propostas por Penman-Monteith (EToPM), Hargreaves & Samani (EToHG) e Blaney-Criddle-FAO (EToBC), para o período de um ano. O objetivo deste estudo foi comparar os métodos de estimativa com EToPM, utilizando parâmetros de regressão (β0, β1), coeficiente de determinação (r²), e correlação (r), estimativa do erro-padrão (SEE), índices de concordância (d) e confiança (c). Os resultados indicam tendência de EToHG à superestimativa dos valores de ETo, exceto na região de Jaú, e subestimativa por EToBC, para todas as regiões estudadas. Nos valores mensais de ETo, os três métodos apresentam a mesma distribuição. O método de EToHG apresenta-se como uma excelente alternativa na estimativa de consumo de água pela cultura.

Técnicas de análise de dados distribuídos em áreas

Pós-graduação em Biometria - IBB

Projeto trilha de letras: uma análise do software como recurso didático no processo ensino-aprendizagem da leitura e da escrita

Pós-graduação em Educação - FCT

Mediação do processo ensino-aprendizagem com o jogo de empresas mercado virtual: uma pesquisa de opinião

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gestão de recursos humanos em projetos de software: proposta integrada ao processo pessoal

The software quality represents a more and more important attribute for the survival and growth of software industries. In order to ensure the quality of products manufactured, various practices have been incorporated into the development process. In this context, some successful software organizations have invested in an approach to human resource management, which consists in integrating the management activities with a personal process called Personal Software Process - PSP, which has led to the maintenance of greater discipline and control over all development phases. Given this, this work presents a proposal for integration of techniques set out in the PSP with a web system previously developed, which is called System to Aid Project Management - SAPM.

Avaliação da hipermídia no processo de ensino e aprendizagem da física: o caso da gravitação

Neste artigo são apresentados os principais resultados obtidos em uma atividade realizada para avaliar um software hipermídia destinado ao ensino e à aprendizagem da Física. Para a realização desta pesquisa, foi desenvolvido, aplicado e avaliado um sistema hipermídia para o ensino de Gravitação, com base nas pesquisas em ensino de Ciências. Foram considerados também os princípios fundamentais que caracterizam a hipermídia enquanto linguagem que permite o acesso não-linear à informação e a apresentação desta com a utilização dos recursos gráficos, sonoros, interativos e de animação do computador, e ainda suas implicações para as práticas de ensino. A maior parte dos professores e dos estudantes envolvidos na pesquisa avaliou positivamente o software quanto a seus aspectos técnicos, pedagógicos e motivacionais. O estudo forneceu evidências de que a hipermídia contribui para a aprendizagem de Física de modo motivador e significativo para os alunos do ensino médio.

HTRIdb: an open-access database for experimentally verified human transcriptional regulation interactions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Definição de requisitos de software baseada numa arquitetura de modelagem de negócios

Não é uma tarefa fácil definir requisitos para os sistemas de software que darão suporte a um negócio, dada a dinâmica de mudanças nos processos. O levantamento de requisitos tem sido feito de forma empírica, sem o apoio de métodos sistematizados que garantam o desenvolvimento baseado nos reais objetivos do negócio. A engenharia de software carece de métodos que tornem mais ordenadas e metódicas as etapas de modelagem de negócios e de levantamento de requisitos de um sistema. Neste artigo é apresentada uma metodologia de desenvolvimento de software resultante da incorporação de atividades propostas para modelagem de negócios e levantamento de requisitos, baseadas em uma arquitetura de modelagem de negócios. Essas atividades tornam o desenvolvimento de software mais sistemático e alinhado aos objetivos da organização, e podem ser incorporadas em qualquer metodologia de desenvolvimento baseada no UP (Unified Process - Processo Unificado).

miRNApath: A database of miRNAs, target genes and metabolic pathways

MicroRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression and hence play important roles in metabolic pathways. Recent studies have evidenced the interrelation of miRNAs with cell proliferation, differentiation, development, and diseases. Since they are involved in gene regulation, they are intrinsically related to metabolic pathways. This leads to questions that are particularly interesting for investigating medical and laboratorial applications. We developed an miRNApath online database that uses miRNA target genes to link miRNAs to metabolic pathways. Currently, databases about miRNA target genes (DIANA miRGen), genomic maps (miRNAMap) and sequences (miRBase) do not provide such correlations. Additionally, miRNApath offers five search services and a download area. For each search, there is a specific type of input, which can be a list of target genes, miRNAs, or metabolic pathways, which results in different views, depending upon the input data, concerning relationships between the target genes, miRNAs and metabolic pathways. There are also internal links that lead to a deeper analysis and cross-links to other databases with more detailed information. miRNApath is being continually updated and is available at http://lgmb.fmrp.usp.br/mirnapath. ©FUNPEC-RP.

A medical information system to manage a cancer database

Cancer is the second main cause of death in Brazil, and according to statistics disclosed by INCA - National Cancer Institute 466,730 new cases of the disease are forecast for 2008. The storage and analysis of tumour tissues of various types and patients' clinical data, genetic profiles, characteristics of diseases and epidemiological data may provide more precise diagnoses, providing more effective treatments with higher chances for the cure of cancer. In this paper we present a Web system with a client-server architecture, which manages a relational database containing all information relating to the tumour tissue and their location in freezers, patients, medical forms, physicians, users, and others. Furthermore, it is also discussed the software engineering used to developing the system.

SKPDB: A structural database of shikimate pathway enzymes

Background: The functional and structural characterisation of enzymes that belong to microbial metabolic pathways is very important for structure-based drug design. The main interest in studying shikimate pathway enzymes involves the fact that they are essential for bacteria but do not occur in humans, making them selective targets for design of drugs that do not directly impact humans.Description: The ShiKimate Pathway DataBase (SKPDB) is a relational database applied to the study of shikimate pathway enzymes in microorganisms and plants. The current database is updated regularly with the addition of new data; there are currently 8902 enzymes of the shikimate pathway from different sources. The database contains extensive information on each enzyme, including detailed descriptions about sequence, references, and structural and functional studies. All files (primary sequence, atomic coordinates and quality scores) are available for downloading. The modeled structures can be viewed using the Jmol program.Conclusions: The SKPDB provides a large number of structural models to be used in docking simulations, virtual screening initiatives and drug design. It is freely accessible at http://lsbzix.rc.unesp.br/skpdb/. © 2010 Arcuri et al; licensee BioMed Central Ltd.