Partial purification, immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus

Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.

Fauna helmintológica de ovinos provenientes da microrregião de Jaboticabal, estado de São Paulo, Brasil

This study evaluated the prevalence and counting parasitism of different species of helminths of sheep from the micro-region of Jaboticabal of Sao Paulo state. For this, 66 animals naturally infected, four to 36 months of age, raised in pasture, were selected. The results of necropsy revealed the presence of seven genera and 12 species with the following prevalence and mean count: Haemonchus contortus: 100.0% (2947.2); Trichostrongylus colubriformis: 90.9% (3048.8); Cooperia curticei: 56.0% (256.5); Oesophagostomum columbianum: 48.4% (36.0); Cooperia punctata: 30.3% (94.5); Trichostrongylus axei: 22.7% (26.5); Strongyloides papillosus: 19.6% (83.0), Haemonchus contortus (L4): 7.5% (17.2), Cooperia pectinata: 10.6% (12.9), Trichuris ovis: 10.6 % (0.6); Cooperia spatulata 4.5% (0.3); Capillaria bovis: 4.5% (0.1). The mean parasitism of helminthswas 6524.7 per animal. Haemonchus contortus (adults and L4) and Trichostrongylus colubriformis corresponded to 45.4% and 46.7% of the average worm burden totally, respectively. Based in the results obtained in this study, can be concluded that the two most abundant species of helminths and important, the micro-region of Jaboticabal are Trichostrongylus colubriformis and Haemonchus contortus, and these two species amounted to 92.1% of the distribution percentage of helminths collected from all animals. These results demonstrate the importance of conducting a counts of eggs per gram of feces (EPG) in the herds of this region when FAMACHA is used on a particular property, since this method control does not allow to diagnostic the damage/clinical signs in animals infected by T. colubriformis, because this specie does not have hematophagism habit on animals.

Characterization and Quantification of the Compounds of the Ethanolic Extract from Caesalpinia ferrea Stem Bark and Evaluation of Their Mutagenic Activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Short-term sustained hypoxia induces changes in the coupling of sympathetic and respiratory activities in rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Degradabilidade de grãos secos e ensilados de sorgo com e sem tanino, submetidos à granulometrias

Due to its nutritional characteristics sorghum has been indicated as substitute cereal to corn and wheat. Besides, in Brazil sorghum is not used for human feeding unlike corn, witch reduces its costs in almost 20% in comparison to corn costs. This research was conducted at UNESP, in Botucatu and Jaboticabal Campus, São Paulo, Brazil, with the purpose to evaluate the grinding effect on two sorghum genotypes grain (with and without tannin) preserved dry or ensiled humid above the degradability in situ of the dry matter, crude protein and starch. Three rumen fistulated sheep were used, distributed in an experimental block design, at 2 x 2 x 3 factorial outline, with two sorghum genotypes (with and without tannin), two forms of conservation (dry grain and high moisture grains silage) and three grinding ways (undivided, thick grinding and fine grinding) and the time as sub-portion. According to the results the grinding is recommended to sorghum grains because it increases the ruminal and effective degradability of the dry matter, crude protein and starch. The presence of tannin in sorghum grain changed the degrability potential of crude protein and starch. The thick grinding in dry grain without tannin was the better treatment.

Metáforas e metamorfoses na poesia de cláudio manuel da costa

This paper explains the physical space allegorization as the main figurative device of the human actor dishumanization theme, in Cláudio Manuel da Costa poetry.

Constância da retórica, mudança de Estilo: a obra acadêmica de Cláudio Manuel da Costa

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A correspondência de Cláudio Manuel da Costa dirigida aos acadêmicos renascidos: informes de uma sociedade letrada

In this article we intend to discuss the type of letters produced by Cláudio Manuel da Costa when he was member of Academia Brasílica dos Renascidos basing the analyses on the ancient rhetoric, used as a model by scholars between sixteenth and eighteenth century. These documents show his filiation with academicism practices, presents evidence of his biography and his domain of two writing styles: the poetic style for which he is known, and the academic style, employed in the exercise of his function under the literate associations, confirming his actions, both in the political and intellectual framework of that time.

Caracterização da polpa de pêssegos produzidos em São Manuel-SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Captura incidental de um tubarão-baleia, Rhincodon typus (Orectolobiformes, Rhincodontidae), na costa do Ceará, Nordeste do Brasil

A whale shark, Rhincodon typus, was incidentally captured off Ceará on January 2009, during gillnet fishery. This is the second occurrence record for this species off the State and the first including material deposited in a scientific collection.