The status of soil phosphate fractions and the ability of fungi to dissolve hardly soluble phosphates

Brazilian soils predominantly consist of iron and aluminum oxides and have a low phosphorus content. The present study was carried out in order to assess the status of phosphate fractions in pasture, forest and agricultural soils and the ability of soil fungi to solubilize iron and aluminum phosphates. The abundance of P fractions in the soils studied occurred in the following order: Fe-P > reductant-soluble Fe-P > occluded Fe-P > occluded Al-P > Al-P > Ca-P. of the 481 fungi isolated, 33 showed the ability to solubilize the inorganic phosphates in culture. of these, 14 were considered to be high or very high solubilizers based on a solubilization capacity > 1000 mu g PO43- ml(-1). Isolate F-111 was the only one that dissolved all the insoluble phosphates used. Nine isolates solubilized both Al-P and Ca-P, and four other isolates only solubilized Ca-P. The highest number of isolates with high solubilization capacity were detected in pasture soil, followed by tropical rain forest and forest patch soils. Pasture soil presented both the largest contents of insoluble phosphates and the largest number of fungal isolates with phosphate-solubilizing ability. The range and size of P fractions influenced the number of fungi and their ability to solubilize hardly soluble phosphates. (c) 2004 Elsevier B.V. All rights reserved.

Crescimento aéreo e radicular de arroz de terras altas em função da adubação fosfatada e bioestimulante

The use of plant regulators that stimulate root growth can increase phosphorus uptake by upland rice. The objective of this study was to evaluate shoot and root growth of upland rice fertilized with different phosphorus doses with and without biostimulant. The experiment was carried out in greenhouse in the Faculdade de Ciencias Agronomicas-UNESP, in Botucatu-SP. The treatments consisted of six phosphorus doses applied in sowing (0, 12,5, 25, 50, 100 and 200 mg dm(-3)), with and without Stimulate (R) applied in the seeds (cv. Primavera). The plants were grown for 78 days and then cut at soil level to evaluate leaf area and leaves and collar dry matter. Root samples that were harvested on the same day had their root diameter and dry matter evaluated. The experimental design was the completely randomized, with three replications, arranged as a factorial 2x6. Variance analysis and regression were used to data evaluation. Linear and quadratic equations were adjusted at a probability level of 5%, using those with higher determination coefficient (R(2)). The increase on the phosphorus dose contributed to the lower matter production and leaf area of the plants when the biostimulant was applied. For shoot phosphorus accumulation and root evaluations, the same behavior was observed. It was concluded that the use of Stimulate (R) in seeds, for fitomass production or root system evaluation, was only efficient in low phosphorus doses.

Adubação fosfatada, componentes de produção, produtividade e qualidade fisiológica em sementes de feijão

O trabalho teve como objetivo avaliar o efeito de doses de fósforo aplicadas via solo, sobre os componentes de produção, produtividade e qualidade fisiológica de sementes de feijão, cv. IAC Carioca. Foram avaliadas seis doses de fósforo (0, 30, 60, 90, 120 e 150kg.ha-1 de P2O5) sob o delineamento experimental em blocos casualizados, com quatro repetições, em Latossolo Vermelho com baixo teor de fósforo (6mg.dm-3), em condições de campo. No momento da colheita, avaliaram-se estande final de plantas, altura da inserção da primeira vagem, comprimento das vagens, número de vagens/planta, número de sementes/vagem, número de lóculos/vagem, número lóculos vazios/vagem, número de lóculos com sementes/vagem e massa de 100 sementes. A produtividade de sementes foi determinada com base na produção das duas linhas centrais de cada parcela, com teor de água corrigido para 13%. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação, primeira contagem de germinação, envelhecimento acelerado, condutividade elétrica, emergência de plântulas no campo e massa de matéria seca de plântulas. A adubação fosfatada com 150kg.ha-1 de P2O5 aumentou o número de vagens/planta e o número de sementes/planta. Os demais componentes de produção e a produtividade de sementes não foram alterados pela aplicação de fósforo. As doses de fósforo não alteraram a qualidade fisiológica das sementes de feijão, cv. IAC Carioca.

Absorção e translocação de fósforo em dois cultivares de mamoneira (Ricinus communis L.), 'Campinas' e 'Guarani', com uso de 32p

Com o objetivo de estudar a absorção e translocação do fósforo na cultura da mamoneira, instalou-se um ensaio em vasos com os cultivares 'Campinas' e 'Guarani' e empregou-se o fósforo radioativo (32p) como traçador. Os quatro tratamentos constaram da aplicação ao solo de 65 ppm de P contendo 32p, quando as plantas apresentavam seis, nove, doze e quatorze folhas, respectivamente e cortadas com nove, doze e quatorze folhas e as do quarto tratamento após 21 días da aplicação do fertilizante marcado. Através dos dados obtidos, pode-se concluir que, neste solo com baixo teor de fósforo, a aplicação deste elemento, na época da emissão de ínflorescências primárias, não influi no desenvolvimento das inflorescências, uma vez que se formam as expensas de fósforo previamente acumulado no pecíolo e no limbo, e que para lá se desloca a fim de participar do metabolismo de formação e desenvolvimento. em outros termos, o fósforo, quando aplicado tardiamente, não incrementará a produção dos cachos primários e, consequentemente, não pode esperar que o potencial máximo do cultivar seja atingido, pois os cachos primários representam, no mínimo, 30% da produção total da mamoneira.

Efeito de diferentes níveis de sódio e fósforo sobre o desempenho e a qualidade da casca dos ovos de poedeiras comerciais

Conduziu-se um experimento com o objetivo de verificar o efeito de diferentes níveis de sódio e fósforo (P) sobre o desempenho e a qualidade da casca dos ovos e de determinados constituintes sangüíneos, em 144 galinhas Hy-Line W36, com 60 semanas de idade, distribuídas em 36 unidades experimentais com quatro aves cada. O delineamento utilizado foi o de blocos ao acaso em arranjo fatorial (3 x 3), níveis de sódio (0,16; 0,20; e 0,24%) e níveis de fósforo total (0,35; 0,45; e 0,55%), totalizando nove tratamentos, dois blocos com duas repetições em cada bloco. As rações experimentais foram isoprotéicas (16,0% de PB), isocalóricas (2800 kcal de EM/kg) e isocálcicas (4,0% de Ca). Consumo de ração, produção e massa de ovos, conversão alimentar, porcentagem e espessura da casca e densidade aparente dos ovos não foram alteradas pelos níveis de sódio. Entretanto, quando as galinhas foram alimentadas com dietas contendo 0,35% de P total, verificou-se comprometimento da produção e massa de ovos e conversão alimentar, sem alteração das características de qualidade da casca. Houve interação entre os fatores para o peso dos ovos. Baixos níveis de P total não foram efetivos em melhorar a qualidade da casca dos ovos, mas prejudicaram o desempenho das galinhas.

Clinical pathological data and analyses of mineral elements of cattle affected by epizootic botulism in the State of São Paulo

In order to study laboratorial aspects of beef cow mortality, a syndrome popularly known as ''doenca da vaca caida'', examens were made of blood, cerebrospinal fluid, serum, bone and liver samples from 32 naturally affected 4 to 9 year old cows, 27 belonging to the Nellore breed and 5 were crossbred Nellore, all originating from farms located in municipalities near Botucatu, State of São Paulo. Laboratory determinations were analysed by descriptive statistics and included hematological values, total plasma protein, plasma fibrinogen, cerebrospinal fluid analysis, and concentration measurements of serum calcium, phosphorus, magnesium, sodium, potassium, chloride, total protein, albumin, globulin, alkaline phosphatase, aspartate aminotransferase, gama-glutamyltransferase and creatine kinase activities, included bone ash percentage and concentrations of calcium, phosphorus and magnesium, and also hepatic levels of copper, zinc, iron, manganese and cobalt. In addition, mouse bioassays and complement micro-fixation tests were performed to detect botulinum toxins in liver samples. The results indicated leukocytosis (13,3+/-3,9 x10(3)/mm(3)) with neutrophilia (8,9+/-3,2 x10(3)/mm(3)), hypocalcemia (7,8+/-1,7mg/dl), hypophosphatemia (3,6+/-1,6mg/dl), hypoalbuminemia (2,9+/-0,9g/dl), increased creatine kinase activity (691,0+/-829,7 UI/1), and reduced ash percentage (60,3+/-1,9%) and low phosphorus (17,2+/-0,4%) in bone. The other values were ail within normal limits. The diagnosis of botulism, involving type C and D toxins, was confirmed as the cause of the mortality in the region of study, what is strongly consistent with the other laboratorial findings.

Alface americana fertirrigada com diferentes doses de ácido fosfórico e hidróxido de potássio

Fertirrigation with phosphoric acid has been considered as an option to avoid drips from clogging. In some cases, this acid cause soil acidification and it can be substituted bypotassium hydroxide. This study had the objective of evaluating the effects of different phosphoric acid and potassium hydroxide doses, applied via fertirrigation, on soil fertility, plant nutrition and lettuce yield, compared to the conventional fertilization system. The experiment was carried out in greenhouse, in the College of Agricultural Sciences, FCA/UNESP, Botucatu-SP-Brazil. The experimental design was the completely randomized block, with 6 treatments and 4 replications. Treatments consisted of the combinations of doses (25% and 50% of the recommended complete dose for the lettuce) and application ofcomplete dose (100%) of phosphorus (P) and potassium (K) applied via fertirrigation, and a control (conventional fertilization system). The results showed that P and K availability in soil and nutrient concentration in plants were affected by treatments. Low phosphorus and potassium doses, applied via fertirrigation, were not efficient for the appropriate development of lettuce plants. The results showed that agronomic characteristics and commercial lettuce were affected by doses and application systems, especially the treatment with application of P and K full dose fertirrigation.

Doses e modos de aplicação de fósforo na nutrição e produção do feijoeiro cultivar Pérola

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento radicular e aéreo, nutrição e eficiência de absorção de macronutrientes e zinco por cultivares de arroz de terras altas afetadas pela adubação fosfatada

In Brazil, phosphorus availability is one factor that that more limit yield of upland rice under rainfed system. Then, better understanding of cultivars development at phosphorus soil fertilization is very important in the production systems making this more sustainable. The objective was to evaluate the influence of phosphorus doses applied to the soil over the root length, root and shoot dry matter, concentration and content of macronutrient and zinc in shoot and root as well as the efficiency of nutrient uptake per meter root of upland cultivars of intermediate and modern groups. The experiment was carried out in greenhouse conditions in a completely randomized design in factorial scheme 4 x 4. Treatments were four levels of P fertilization applied at the soil (0, 50, 100 and 200 mg dm(-3) of P) and four upland rice cultivars (Maravilha - modern group, IAC-201, IAC-202 and Carajas - intermediate group). There is increase in phosphorus availability in the soil with increasing levels of P fertilization. Under low phosphorus availability, cultivars of the intermediate group have better shoots and root system development in relation to the modern cultivar group. Level of phosphorus affected nutrients contents in shoots and root system of upland rice cultivars. The increasing phosphorus fertilization increased uptake of nutrients per meter of root; and although under higher phosphorus availability there was a greater root growth, in low phosphorus availability root growth was greater at the expense of shoot growth.

Aspectos Nutricionais na Doença Renal Crônica em Cães

It is understood by Chronic Renal Failure (CRF) or Stage IV Chronic Kidney Disease (CKD) the morbid state in which there is a substantial and irreversible loss of functional capacity, metabolic and endocrine function in both kidneys, leading to a framework of dehydration, azotemia, electrolyte imbalance, anemia, uremic syndrome, secondary hyperparathyroidism, among other conditions that make the patient's life without treatment unfeasible. An adequate nutritional support to patients with CKD at any stage aims to maintain a good clinical condition and an attempt to delay the progression of the disease. The basis of a diet for patients with CKD is the restriction in this protein being of high biological value, low-phosphorus, high energy density, fiber and antioxidants combined with a good dietary practices that allow the patient a good quality of life

Organic compounds from plant extracts and their effect on soil phosphorus availability

O objetivo deste trabalho foi avaliar o efeito de compostos orgânicos de extratos de plantas de seis espécies e da fertilização fosfatada na disponibilidade de fósforo no solo. Tubos de 30 cm de altura e 5 cm de diâmetro foram preenchidos com Latossolo Vermelho-Amarelo. Cada tubo constituiu uma parcela, em delineamento completamente casualizado, em arranjo fatorial 7x2, com quatro repetições. Extratos líquidos de aveia-preta (Avena strigosa), nabo forrageiro (Raphanus sativus), milho (Zea mays), milheto (Pennisetum glaucum), soja (Glycine max), sorgo forrageiro (Sorghum bicolor) e água (testemunha) foram aplicados em cada parcela, com ou sem fertilização com fosfato solúvel. Após sete dias de incubação, amostras de solo foram coletadas em várias profundidades, e foram analisadas as formas lábil, moderadamente lábil e não lábil de fósforo no solo. Houve acúmulo de fósforo inorgânico nas frações lábil e moderadamente lábil do solo, como conseqüência da adição dos extratos de plantas, principalmente na camada superficial (0-5 cm). O nabo forrageiro, com maior concentração de ácido málico e maior conteúdo de P no tecido do que outras espécies, foi o mais eficiente em incrementar a disponibilidade de P no solo.

Doses and forms of application of phosphorus in vernalized garlic

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Feed digestibility and productive performance of bullfrogs fed in high and low frequency

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vitro evaluation of acidified toothpastes with low fluoride content

Fluoride toothpastes are a risk factor for the development of dental fluorosis. Products with low fluoride content offer a higher security, but their effectiveness must be proven. The aim of this in vitro study was to compare two acidified toothpastes with low fluoride concentration (412 and 550 mu g F/g) with neutral toothpastes. Bovine enamel blocks were selected by surface microhardness (SMH) and randomized to twelve groups of 13, according to the fluoride concentration in toothpaste (placebo, 275, 412, 550 or 1,100 jig F/g) and pH (7.0 or 5.5). Two commercially available toothpastes were also studied: a 1,100-mu g F/g, pH 7.0 paste (positive control) and a children's paste (500 mu g F/g, pH 7.0). The blocks were subjected to pH cycling for 7 days. The toothpaste treatment was done twice daily. Surface and cross-sectional microhardnesses were assessed to calculate the percentage change of SMH (%SMH) and the mineral loss (Delta Z). The amount of fluoride, calcium and phosphorus in the solutions after the pH cycling was also analyzed. Compared to neutral toothpastes, the acidified toothpastes reduced the %SMH in all F concentrations. Higher F and lower Ca and P concentrations were found in solutions for the acidified toothpastes. Regarding AZ, only the positive control, 1,100-mu g F/g (acidified and neutral) groups were not statistically different. The acidified toothpastes showed a dose-response relationship with all variables. For the low-fluoride toothpastes evaluated, only the 550-mu g F/g acidified paste had the same anticariogenic action as the 1,100-mu g F/g neutral paste.

Tillage and phosphorus management effects on enzyme-labile bioactive phosphorus availability in Cerrado Oxisols

Phosphorus (P) is an essential element in crop nutrition, which can be growth limiting or an environmental contaminant, if present in excess. Tillage practices have a direct effect on the behavior and availability of soil P. Sorption and availability of various P forms were evaluated in an incubation-fractionation study of three soils, a Typic Paleudults (CR soil) and two Cerrado Oxisols (Latossolo Vermelho-Amarelo [LVA] and Latossolo Vermelho [LV]) with distinct biogeochemical characteristics and tillage management history. Phosphate and myo-inositol hexakisphosphate (mIPH) were strongly sorbed by the soils. Maximum adsorption capacities (S(max)) were 2.2-6.9, 3.3-7.8, and 1.6-19.8 mmol kg(-1) for phosphate in the 0-40 cm depths of the CR, LV, and LVA soils, respectively. For mIPH, S. were 1.2-3.7, 3.7-5.5, and 4.6-5.2 mmol kg(-1). Saturation indices reflected the long-term effect of repeated manure applications on the Paleudults and the near saturation of its P holding capacity, in contrast to the recently cultivated Cerrado soils. Tillage method appeared to have altered P retention characteristics of the near-surface zone very slightly, while increases in ligand-exchangeable (EEP;) and enzyme-labile organic P (EDTA-PHP) forms were observed in no-till Oxisols. In the Paleudults, added manure P increased bioactive P fractions and P saturation of no-till near-surface soil zone. Estimates of all bioactive P fractions using the ligand-based enzymatic assay showed it to be an effective method for assessing P availability in soil and developing sustainable P management strategies, particularly in Cerrado Oxisols that were low in organic matter while having an extensive P-fixing capacity. Published by Elsevier B.V.