A Sorocabana Railway Company: a relação de uma empresa ferroviária privada com as diretrizes governamentais (1907-1919)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparison Between the Polymerase Chain Reaction-Based Screening and the Southern Blot Methods for Identification of Fragile X Syndrome

The fragile X syndrome (FXS), the most common cause of hereditary mental retardation, is caused by expansions of CGG repeats in the FMR1 gene. The gold-standard method to diagnose FXS is the Southern blot (SB). Because SB is laborious and costly, some adaptations in the polymerase chain reaction (PCR) method have been utilized for FXS screening. A previous PCR-based screening method for FXS identification utilizing small amounts of DNA was reported as simple and efficient. The aim of this study was to reproduce the mentioned PCR-based screening method for identification of expanded alleles of the FMR1 gene in Brazilian individuals and to investigate the efficiency of this method in comparison with SB. Utilizing the enzyme Expand Long Template PCR System, 78 individuals were investigated by that PCR-based screening method for FXS identification. Conclusive results were obtained for 75 samples. Considering all the allelic forms of FXS (normal [NL], premutation [PM], and full-mutation [FM]), the comparison of the PCR-based screening method with SB demonstrated 100% of accuracy, sensitivity, and specificity. However, when the PM and the FM were analyzed separately from each other, but together with the NL allele, the accuracy, sensitivity, and specificity decreased (to 42.9%-97.4%). We concluded that the PCR-based screening method was reproducible and capable of identifying all different FXS alleles, but because the differentiation between the PM and the FM alleles was not accurate, SB is still the gold-standard method for the molecular diagnosis of FXS.

The importance of Leptospira interrogans serovars Icterohaemorrhagiae and Canicola in coastal zone and in southern fields of Rio Grande do Sul, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Modelling the surface circulation and thermal structure of a tropical reservoir using three-dimensional hydrodynamic lake model and remote-sensing data

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Maturation and growth curves of Macrobrachium Carcinus (Linnaeus) (Crustacea, Decapoda, Palaemonidae) from Ribeira de Iguape River, southern Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Contamination by chlorinated pesticides, PCBs and PBDEs in Atlantic spotted dolphin (Stenella frontalis) in western South Atlantic

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous filamentous organisms

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cyanobacteria from coastal lagoons of Southern Brazil: coccoid organisms

Considering the great ecological importance of the cyanobacteria and the need for more detailed information about these organisms in Brazilian waters, this paper provides taxonomic information about the unicellular cyanobacteria flora in lagoon systems along the coastal plains of Rio Grande do Sul State. Sampling was performed in different freshwater bodies along the eastern (Casamento Lake area) and western (near the city of Tapes) banks of the Patos Lagoon (30º40' S-30º10' S and 50º30' W-51º30' W). The samples were collected once in the rainy season and once in the dry season (from May 2003 to December 2003) using a plankton net (25 µm mesh) in pelagic and littoral zones, and by squeezing the submerged parts of aquatic macrophytes. Thirty one species belonging to the families Synechoccocaceae (7 taxa), Merismopediaceae (12 taxa), Chamaesiphonaceae (1 taxon), Microcystaceae (4) and Chroococcaceae (7 taxa) were identified. Among these species, five are reported for the first time in Rio Grande do Sul State: Chamaesiphon amethystinus (Rostafinski) Lemmermann, Chroococcus minimus (Keissler) Lemmermann, Coelomoron pusillum (Van Goor) Komárek, Coelosphaerium kuetzingeanum Naegeli, and Cyanodictyon tubiforme Cronberg.

Benthic foraminiferal distribution on the southeastern Brazilian shelf and upper slope

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The invasion of five alien species in the Delta do Parnaiba Environmental Protection Area, Northeastern Brazil

Marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. In the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species) were detected during dives, shore searches and from the fisheries on the coast of the Delta do Parnaiba Environmental Protection Area, in the States of Piaui and Maranhao, Northeastern Brazil. The species were the bicolor purse-oyster Isognomon bicolor, the whiteleg shrimp Litopenaeus vannamei, the giant river prawn Macrobrachium rosenbergii, the Indo-Pacific swimming crab Charybdis hellerii and, the muzzled blenny Omobranchus punctatus. Ballast water (I. bicolor, C. hellerii, and O. punctatus) and aquaculture activities (L. vannamei and M. rosenbergii) in adjacent areas are the most likely vectors of introduction. All exotic species found have potential impact risks to the environment because they are able to compete against native species for resources (food and habitat). Isognomon bicolor share the same habitat and food items with the native bivalve species of mussels and barnacles. Litopenaeus vannamei share the same habitat and food items with the native penaeids such as the pinkspot shrimp Farfantepenaeus brasiliensis, the Southern brown shrimp Farfantepenaeus subtilis, and the Southern white shrimp Litopenaeus schmitti, and in the past few years L. vannamei was responsible for a viral epidemics in the cultivation tanks that could be transmitted to native penaeid shrimps. Charybdis hellerii is also able to cause impacts on the local fisheries as the species can decrease the populations of native portunid crabs which are commercialized in the studied region. Macrobrachium rosenbergii may be sharing natural resources with the Amazon River prawn Macrobrachium amazonicum. Omobranchus punctatus shares habit with the native redlip blenny Ophioblennius atlanticus and other fishes, such as the frillfin goby Bathigobius soporator. Some immediate remedial measures to prevent further introductions from ballast water and shrimp farm ponds should be: (i) to prevent the release of ballast water by ship/vessels in the region; (ii) to reroute all effluent waters from shrimp rearing facilities through an underground or above-ground dry well; (iii) to install adequate sand and gravel filter which will allow passage of water but not livestock; (iv) outdoor shrimp pounds located on floodable land should be diked, and; (v) to promote environmental awareness of those directly involved with ballast water (crews of ship/vessels) and shrimp farms in the region. Rev. Biol. Trop. 58 (3): 909-923. Epub 2010 September 01.

Natural and artificially-induced habitat complexity and freshwater fish species composition

The effects of habitat complexity, induced by construction of artificial reefs, on the fish assemblages in the Barra Bonita reservoir, Brazil, and in the lotic zone immediately below the dam were studied. Four artificial reefs were constructed in each habitat at variable distances from the shore. Multiple correspondence analysis showed that the factors distance from the shore and type of habitat were determinants for the group formation, and artificial reefs had a lesser effect. Fish species composition was about the same at locations with and without reefs.

Diversity and distribution of crustaceans from 13 sheltered sandy beaches along Sao Sebastiao Channel, south-eastern Brazil

The crustacean species composition in the intertidal zones of 13 sheltered unconsolidated marine beaches in south-eastern Brazil is described. Fifty-three crustacean species were collected, adding 46 species to the total reported by previous studies in the same region. Decapods dominated the community, in contrast to exposed sandy beaches where peracarids normally predominate. The species were distributed irregularly among the beaches. Richness varied markedly among sites, and was positively related to a combination of factors such as fine sand grains, high organic matter content, and relatively low silt-clay content. The presence of rock fragments enabled both rocky shore and sandy beach crustaceans to occur oil the same beaches. Richness and abundance of crustaceans showed no clear relationship to sediment grain size and slope, in contrast to the norm for exposed sandy beaches. The dominance of the tanaid Kalliapseudes schubarti in sonic areas may be a result of organic matter pollution in the region. These beaches showed higher species richness than typical sheltered and exposed sandy beaches, indicating that this sheltered, highly heterogeneous seascape is an important area for conservation.

Ecology and reproductive biology of two species of Aplastodiscus (Anura : Hylidae) in the Atlantic forest, Brazil

The present study was conducted in the Serra do Japi in the State of São Paulo, Brazil. From March 2004 to January 2006 we studied the ecology and reproductive biology of two sympatric species of Aplastodiscus in three different habitats: lake, stream, and swamp. The majority of A. leucopygius males in calling activity were recorded during the rainy season and during sporadic rains in the dry season (April to September). The same was observed for A. arildae. Most courtship displays of A. leucopygius were observed mainly during the rainy period and only one courtship behaviour was observed for A. arildae during a rainy night, in December 2005. Aplastodiscus leucopygius males were found in activity in the three habitats surveyed. In contrast, males of A. arildae were found in only one habitat (stream). Spatial distribution seems to be the main mechanism of reproductive segregation between the studied species.

Changes in coral reef communities and an associated reef fish species, Cephalopholis cruentata (Lacepede), after 30 years on Curacao (Netherlands Antilles)

Using the same methodology and identical sites, we repeat a study dating from 1973 and quantify cover of hard coral species, soft corals, sponges, hard substratum and soft substratum, and density of a commercially important reef fish species, the graysby Cephalopholis cruentata, along a depth-gradient of 3-36 m oil the coral reefs of Curacao. The objective was to determine the multi-decade change in benthic coral reef cover and structural complexity, and their effect oil densities of an associated reef fish species. Total hard coral cover decreased on average from 52% in 1973 to 22% in 2003, representing a relative decline of 58%. During this time span, the cover of hard substratum increased considerably (from 11 to 58%), as did that of soft corals (from 0.1 to 2.2%), whereas the cover of sponges showed no significant change. Relative decline of hard coral cover and of reef complexity was greatest in shallow waters (near the coast), which is indicative of a combination of anthropogenic influences from shore and recent storm damage. Cover of main reef builder coral species (Agaricia spp., Siderastrea siderea, Montastrea annularis) decreased more than that of other species, and resulted in a significant decrease in reef complexity. Although density of C. cruentata was highly correlated to cover of Montastrea and Agaricia in 1973, the loss of coral cover did not show any effect on the total density of C. cruentata in 2003. However, C. cruentata showed a clear shift in density distribution from shallow water in 1973 to deep water in 2003. It call be concluded that the reefs of Curacao have degraded considerably in the last three decades, but that this has had no major effect on the population size of one commercially important coral-associated fish species.

Transmission-expansion planning using the DC model and nonlinear-programming technique

The paper presents a constructive heuristic algorithm (CHA) for solving directly the long-term transmission-network-expansion-planning (LTTNEP) problem using the DC model. The LTTNEP is a very complex mixed-integer nonlinear-programming problem and presents a combinatorial growth in the search space. The CHA is used to find a solution for the LTTNEP problem of good quality. A sensitivity index is used in each step of the CHA to add circuits to the system. This sensitivity index is obtained by solving the relaxed problem of LTTNEP, i.e. considering the number of circuits to be added as a continuous variable. The relaxed problem is a large and complex nonlinear-programming problem and was solved through the interior-point method (IPM). Tests were performed using Garver's system, the modified IEEE 24-Bus system and the Southern Brazilian reduced system. The results presented show the good performance of IPM inside the CHA.