Armazenamento de sementes de carolina em diferentes temperaturas e embalagens

O objetivo do presente trabalho foi determinar a embalagem e a temperatura adequadas para o armazenamento de sementes de carolina. As sementes foram armazenadas em sacos de papel e de plástico, mantidas em 0±2; 10±2; 20±2°C e 60 5% de umidade relativa do ar (UR) e em temperatura ambiente (23,4±3,3°C) e 68,7±9%UR. O teor de água, a germinação e o vigor foram determinados trimestralmente. Durante o armazenamento, o teor de água das sementes foi de aproximadamente 8,9%. A embalagem saco de plástico e a temperatura de 0°C são adequadas para o armazenamento das sementes de carolina.

The megalopa and early juvenile stages of Calappa tortugae Rathbun, 1933 (Crustacea, Brachyura) reared in the laboratory from South Carolina neuston samples

Neuston samples collected from the Charleston Bump region off the coast of South Carolina, U.S.A., during the summers of 2002 and 2003 consistently included a decapod species of undetermined identity with a large brachyuran megalopa. Despite their resemblance to some calappids, it was impossible to make a definitive identification based solely on general morphology. Therefore, additional neuston tows were taken on the continental shelf near Charleston, during the summer of 2004 to obtain these living megalopae. These were raised successfully through five juvenile stages at the Southeastern Regional Taxonomic Center (SERTC) laboratory. The morphology of the juveniles provided evidence that they are megalopae of Calappa tortugae Rathbun, 1933. Comparisons with megalopae of Hepatus epheliticus (Linnaeus, 1763), H. pudibundus (Herbst, 1785), Calappa flammea (Herbst, 1794) and Cryptosoma balguerii (Desbonne, 1867) are presented here. This is the first complete description of the megalopa morphology of a member of the genus Calappa Weber, 1795 from the Western Atlantic, and it is helpful for taxonomic, systematic and ecological purposes.

Genetic diversity of Rhizoctonia solani AG-3 from potato and tobacco in North Carolina

Anastomosis group 3 (AG-3) of Rhizoctonia solani (teleomorph = Thanatephorus cucumeris) is frequently associated with diseases of potato (AG-3 PT) and tobacco (AG-3 TB). Although isolates of R. solani AG-3 from these two Solanaceous hosts are somatically related based on anastomosis reaction and taxonomically related based on fatty acid, isozyme and DNA characters, considerable differences are evident in their biology, ecology, and epidemiology. However, genetic diversity among field populations of R. solani AG-3 PT and TB has not been documented. In this study, the genetic diversity of field populations of R. solani AG-3 PT and AG-3 TB in North Carolina was examined using somatic compatibility and amplified fragment length polymorphism (AFLP) criteria. A sample of 32 isolates from potato and 36 isolates from tobacco were paired in all possible combinations on PDA plus activated charcoal and examined for their resulting somatic interactions. Twenty-eight and eight distinct somatic compatibility groups (SCG) were identified in the AG-3 PT and AG-3 TB samples, respectively. AFLP analyses indicated that each of the 32 AG-3 PT isolates had a distinct AFLP phenotype, whereas 28 AFLP phenotypes were found among the 36 isolates of AG-3 TB. None of the AG-3 PT isolates were somatically compatible or shared a common AFLP phenotype with any AG-3 TB isolate. Clones (i.e., cases where two or more isolates were somatically compatible and shared the same AFLP phenotype) were identified only in the AG-3 TB population. Four clones from tobacco represented 22% of the total population. All eight SCG from tobacco were associated with more than one AFLP phenotype. Compatible somatic interactions between AG-3 PT isolates occurred only between certain isolates from the same field (two isolates in each of four different fields), and when this occurred AFLP phenotypes were similar but not identical.

Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.

Detecting migrants in populations of Rhizoctonia solani anastomosis group 3 from potato in North Carolina using multilocus genotype probabilities

The relative contribution of migration of Rhizoctonia solani anastomosis group 3 (AG-3) on infested potato seed tubers originating from production areas in Canada, Maine, and Wisconsin (source population) to the genetic diversity and structure of populations of R. solani AG-3 in North Carolina (NC) soil (recipient population) was examined. The frequency of alleles detected by multilocus polymerase chain reaction-restriction fragment length polymorphisms, heterozygosity at individual loci, and gametic phase disequilibrium between all pairs of loci were determined for subpopulations of R. solani AG-3 from eight sources of potato seed tubers and from five soils in NC. Analysis of molecular variation revealed little variation between seed source and NC recipient soil populations or between subpopulations within each region. Analysis of population data with a Bayesian-based statistical method previously developed for detecting migration in human populations suggested that six multilocus genotypes from the NC soil population had a statistically significant probability of being migrants from the northern source population. The one-way (unidirectional) migration of genotypes of R. solani AG-3 into NC on infested potato seed tubers from Canada, Maine, and Wisconsin provides a plausible explanation for the lack of genetic subdivision (differentiation) between populations of the pathogen in NC soils or between the northern source and the NC recipient soil populations.

Caracterização físico-química de galactomananas de Adenanthera pavonina (Carolina) e Delonix regia (Flamboyant) reticuladas com trimetafosfato de sódio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mulher e negra: as memórias de Carolina Maria de Jesus e Maya Angelou

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diários íntimos e literatura: a poesia de Carolina

Pós-graduação em Letras - FCLAS

Avaliação do estresse em cordeiros submetidos a quatro métodos de caudectomia: Lívia Carolina Tomazini Peres. -

Pós-graduação em Ciência Animal - FMVA

Maturação sexual em bezerras nelore com suplementação alimentar: Maria Carolina Villani Miguel. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Saberes geográficos integrados aos estudos territoriais sob a ótica da implantação do Parque Nacional da Chapada das Mesas, sertão de Carolina – MA

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudos da lesão ao DNA por corantes têxteis e da capacidade protetora de flavonóides empregando biossensor eletroquímico: Carolina Venturini Uliana. -

Pós-graduação em Química - IQ

Percursos de uma poética de resíduos na obra de Carolina Maria de Jesus

A finalidade desse trabalho é discutir a problemática acerca da tensão produzida por variações de discursos, que cedem forma à experiência narrativa da “poeta do lixo” Carolina Maria de Jesus em seu diário/romance/reportagem Quarto de despejo: diário de uma favelada (1960). Entendemos que sua literatura é marcada por uma mistura de estilos literários desterritorializados, sendo capaz de ativar uma linha de fuga em relação às literaturas canonizadas. Nisso incide a caracterização desse tipo de composição artística, comum em países de formações culturais híbridas. Observamos nesse texto a presença de uma potência rizomática que consiste justamente na reformulação de elementos que compõem esta poética de resíduos justapostos numa certa “deformidade” estilística para além de livros em registro. Notamos que, assim como a escritora reciclava lixo para comer, também o fazia com os discursos de sua poética. Renova por tentar administrar e, conseqüentemente, reestruturar os sentidos de nosso sistema social, no campo da arte escrita, de maneira intuitiva pois, como sabemos, não possuía um projeto literário como os escritores tradicionais.

Cartografando uma literatura menor: a poética dos resíduos de Carolina Maria de Jesus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Do criador à criatura: o desdobramento discursivo em Fazendo Ana Paz e Retratos de Carolina de Lygia Bojunga

No presente estudo, focalizamos as narrativas Fazendo Ana Paz (1991) e Retratos de Carolina (2002) de Lygia Bojunga, verificando os modos como a autora incorpora a materialização da autoria implícita, ou seja, a projeção da categoria autoral no universo diegético, instaurando, assim, um jogo mimético entre realidade e ficção.