Actively transcribed GAL genes can be physically linked to the nuclear pore by the SAGA chromatin modifying complex

Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the nuclear periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of the inner nuclear basket of the nuclear pore that mediate interactions with these active genes. To investigate the physical link between the NPC and active loci, we identified proteins that interact with the carboxyl-terminal globular domain of Mlp1 by tandem affinity purification coupled with mass spectrometry. This analysis led to the identification of several components of the Spt-Ada-Gcn5-acetyltransferase ( SAGA) histone acetyltransferase complex, Gcn5, Ada2, and Spt7. We utilized co-immunoprecipitation and in vitro binding assays to confirm the interaction between the Mlp proteins and SAGA components. Chromatin immunoprecipitation experiments revealed that Mlp1 and SAGA components associate with the same region of the GAL promoters. Critically, this Mlp-promoter interaction depends on the integrity of the SAGA complex. These results identify a physical association between SAGA and the NPC, and support previous results that relied upon visualization of GAL loci at the nuclear periphery by microscopy ( Cabal, G. G. Genovesio, A., Rodriguez-Navarro, S., Zimmer, C., Gadal, O., Lesne, A., Buc, H., Feuerbach- Fournier, F., Olivo-Marin, J.-C., Hurt, E. C., and Nehrbass, U. ( 2006) Nature 441, 770-773). We propose that a physical interaction between nuclear pore components and the SAGA complex can link the actively transcribed GAL genes to the nuclear pore.

O romance, leituras da história: a saga de Cristóvão Colombo em terras americanas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Refiguração do tempo histórico pela ficção

M. Bakhtin afirma que a representação de acontecimentos, relações e processos no romance deve abranger a totalidade de uma época nela deve haver uma peculiar concatenação, literariamente expressa, espácio-temporal. Essa formulação contém elementos basilares para o entendimento e análise do romance, objeto deste estudo, Leite derramado de 2009 de Chico Buarque. A narrativa figura, pelo menos, duas grandes épocas históricas do Brasil: uma que vai do início do século XIX até 1930 e outra que vai desse momento ao início do XXI. É a representação da saga histórica da decadência de uma categoria social (fração da classe dominante), personificada na família Assumpção, com seus valores éticos e culturais, suas concepções de mundo e seu comportamento, estilizando sua dissolução social e moral. Ao fazer isso o romance coloca inúmeros problemas desde humano-existenciais até histórico-políticos para a reflexão do leitor.

Valores Referenciais para Procedimentos Odontológicos (VRPO) em Ortodontia

Introduction: The cost of orthodontic treatment is a very important issue. The aim of this study was to analyze the current Indicative Values for Dental Procedures (IVDP) for orthodontic procedures. Methods: Laws, dental entities papers and scientific literature were utilized. Results: Laws are too general and only offer abstract parameters when define remunerations. Current IVDP vary in the same State, according to the consultant entity. Dental entities interpret that general clinicians can practice Corrective Orthodontics, but new scientific studies begin to question this understanding. The Brazilian Association of Orthodontics and Dentofacial Orthopedics (ABOR) suggests that IVDP for orthodontic specialty must be formulated. Conclusion: Current IVDP are not standardized and are below the correct values for orthodontic procedures, because they are the mean of general clinicians and orthodontists remunerations; and ABOR is legally and ethically supported to claim specific IVDP for orthodontic specialty.

O teatro político de rua praticado pelos coletivos ALMA e Dolores: estéticas de combate e semeadura

Pós-graduação em Artes - IA

A dramaturgia do trabalho no teatro paulistano contemporâneo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cais da Sagração e a sua tradução para o inglês: uma análise de elementos estruturais e culturais da narrativa

Pós-graduação em Letras - IBILCE

Arte sem fronteiras. Londres, Berlim, São Paulo e Rio de Janeiro: olhares estéticos e capitalistas sobre as células do poder lícito e ilícito (séculos XVIII e XX)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hilda Hilst e o seu pendulear em Fluxo-floema

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Silvino Jacques: interseções no mito do bandoleiro

Pós-graduação em Letras - IBILCE

Os ídolos nas revistas teen: uma análise de Atrevida, Capricho e Todateen

This study aims to conduct a comparative analysis of the cover stories of magazines Atrevida, published by Escala, Capricho, published by Abril, and Todateen, published by AltoAstral, published between 2008 and 2012, which give prominence to the phenomena with more recurring presence on the covers. The objective of the research is to analyze the criteria for newsworthiness adopted by publications targeting a teenage audience, relating them to cover highlights involving idols of that audience. As object, we study the press coverage given to four magazines phenomena of cultural industry: the actors in the Twilight saga, the actors of the Disney Channel series, singer Justin Bieber and actors of Brazilian soap opera Rebelde. The work begins with a history of magazine journalism, from its origin to the journalism geared toward teenage readers, through the history of women's magazines and variety. Then, we present the theoretical concepts from the Theory News (Traquina) in confrontation with the notions of consumption (Canclini) and behavior (Featherstone). Finally, analysis of the corpus, which was defined by a cutout thematic within this period, divided into two major groups of news reports: those in which the idol is the news, and those in which he serves as a guide for the reader

Análise estatística de parâmetros elétricos em usinas sucroalcoleiras do centro-oeste paulista

The industry of sugar cane has become an important contributor to the generation of electricity in Brazil. Currently there are 434 sugar and ethanol plants operating in the country, 23% of the total export electricity to the National Integrated System (SIN), the state of São Paulo has 182 plants and 30% of them export energy to the SIN. The objective of this study is to compare parameters of electrical efficiency in the sugar and alcohol industry. For the study, three plants localized in the midwest region of Sao Paulo state with great potential for production and exporting bioenergy were chosen. Five energy analyzers LANDYS + GYR SAGA were used for measure the electrical parameters. The variables studied were energy consumption (C) and power factor (PF). For the statistical analysis it was adopteda randomized block design in a factorial 3 5composed of three companies and five sectors of energy consumption,in which: reception(1), milling (2), boiler (3), supporting activities / juice treatment (4), and distillation (5), totaling 15 treatments. Each group comprised 192 repetitions (48 hours 4 measurements per hour). It was concluded that there is no interest for the plans to fix the FP and reach a value 0.92, which is considered the ideal power factor.This,because the plants generate their own energy and are not penalized. Regarding the energy consumed, all sectors had significant differences. When comparingsector to sector, the plant called USB showed no significant differences in sectors 1 and 3, and the plant USC, in sectors 1 and 4. Considering the production units of this sector and selling power this type of evaluation is essential to perform this analysis, since the analyzed sectors are most important in the production of sugar and ethanol, and analyze and monitor these parameters, use and consumption energy can provide a greater supply of energy to be commercialized.