Resistência de variedades de algodão ao curuquerê do algodoeiro Alabama argillacea Hubner (lepidoptera: noctuidae)

Este trabalho teve como objetivo verificar a ocorrência das formas de resistência, tais como: a não preferência para oviposição, a não preferência para alimentação e a antibiose, em variedades de algodoeiro (Gossypium hirsutum L.), à Alabama argillacea (Hubner, 1818), em experimentos de laboratório (27 ± 2 ºC, 70 ± 10% de U.R. e fotofase de 14 horas). Para avaliar a não preferência para oviposição e para a alimentação em teste com chance de escolha, adotou-se o delineamento em blocos casualizados e, no teste sem chance, o inteiramente casualizado. Para avaliar a antibiose, foram fornecidas às larvas, diariamente, folhas das variedades DeltaOpal, NuOpal, FMT 701, FMX 910 e 20 FMX 996. Durante este processo, verificou-se a duração de cada fase do inseto, avaliando-se a massa de larvas aos dez dias de idade, massa de pupas com 24 horas, período larval, pré-pupal e pupal, longevidade de adultos, viabilidade total e fecundidade. As variedades NuOpal e FMT 701 apresentam resistência do tipo não preferência para alimentação. As variedades NuOpal, FMT 701 e FMX 910 apresentam resistência do tipo antibiose em relação a A. argillacea.

A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hubner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Do Different Casein Concentrations Increase the Adverse Effect of Rutin on the Biology of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae)?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

MORTALITY OF ANTICARSIA GEMMATALIS (LEPIDOPTERA: NOCTUIDAE) CATERPILLARS POST EXPOSURE TO A COMMERCIAL NEEM (AZADIRACHTA INDICA, MELIACEAE) OIL FORMULATION

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Second generation European corn borer injury and Irish potato physiology, yield, and quality

European corn borer (ECB) [Ostrinia nubilalis (Hubner)] (Lepidoptera: Crambidae) is known to infest Irish potato (Solanum tuberosum L.) but only causes economic damage during the first generation in East Coast potato producing areas. However, in Nebraska, second generation ECB infest potato plants during the bulking period and may reduce yield and/or potato quality. Experiments were conducted in 2001, 2002, and 2003 to examine physiological and yield effects of second generation ECB injury to potato in Nebraska. Pike, Atlantic, and three Frito Lay proprietary varieties (FL1867, FL1879, and FL1833) were used. Experimental plots were infested with four ECB egg masses per plant to simulate ECB infestation by second-generation larvae; controls received no egg masses. Photosynthetic rates, tuber weights, tuber size grades, solids, and fry quality were measured. Potato plants with ECB infestation had significantly reduced photosynthetic rates on ECB-infested stems and on uninfested stems on the same plant when larvae were in the fifth instar. When insects were in the fourth instar, photosynthetic rates were reduced only on ECB-infested stems. In 2001, ECB infestation reduced the average mass of large tubers and increased the amount of small tubers in FL1867 and FL1879. In 2002, significant yield reductions were not observed. Across both years, ECB-infested plots produced fewer large (65- to 100-mm diam.) tubers than control plots. Other tuber properties and chip qualities were unaffected. This study indicates that second generation ECB infestation of approximately 30% infested plants results in economic loss for some chipping varieties and affects tuber bulking. In contrast to east coast growers, Midwest potato farmers must be concerned with second generation ECB.

Dinâmica populacional de ovos e lagartas e parasitismo de ovos de Curuquerê-do-algodoeiro por Trichogramma pretiosum em cultivares convencionais e transgênica de algodoeiro

The introduction of new cotton cultivars in the Midwest region of Brazil resulted in a significant increase in productivity, but the use of inappropriate farming techniques brought many problems to field, as the higher incidence of pests, diseases and weeds. The aim of this work was to study the population dynamics of eggs and larvae of cotton leafworm and natural egg parasitism of the pest by Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) at different phenological stages of conventional and transgenic cultivars (Bollgard I) of cotton was carried out this experiment from December 2007 to April 2008 in Ipameri, Goias State, Brazil. The experimental design was a randomized blocks with five trataments and four replications. The treatments consisting of the conventional cotton cultivars DeltaOPAL, FMX 966, FMX 993, FMX 910 and the cultivar transgenic NuOPAL. Allabama argillacea Hubner (Lepidoptera: Noctuidae) oviposited on all cultivars, not presented differences in relation to oviposition preference. Compared to the average number of eggs of A. argillacea parasitized by T. pretiosum, there were no differences between cultivars. In conventional cultivars, small, medium and large larvae occurred from 34 days after plant emergence until the end of the cycle, while in the transgenic cultivar were found only small caterpillars. Cultivar NuOPAL control cotton leafworm since the first larval stage, and does not interfere in egg parasitism by T. pretiosum compared with other cultivars.

Immunity of an Alternative Host Can Be Overcome by Higher Densities of Its Parasitoids Palmistichus elaeisis and Trichospilus diatraeae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Toxicity of extracts and isobutyl amides from Piper tuberculatum: potent compounds with potential for the control of the velvetbean caterpillar, Anticarsia gemmatalis

The isobutyl amides pellitorine (compound 1) and 4,5-dihydropiperlonguminine (compound 2) were extracted from the seeds of Piper tuberculatum Jacq. (Piperaceae) in yields of 6.10 and 4.45% respectively. The acute toxicities to the velvetbean caterpillar, Anticarsia gemmatalis (Hubner) (Lepidoptera: Noctuidae), of extracts of seeds, leaves and stems of P. tuberculatum, and of compounds 1 and 2, were evaluated by means of contact bioassays. The extracts caused 80% mortality when doses higher than 800.00 mu g insect(-1) of extract of seeds, leaves and stems were administered to the velvetbean caterpillars. Compounds I and 2 showed 100% mortality at doses of 200 and 700 mu g insect(-1) respectively. The LD50 and LD90 values were respectively 31.3 and 104.5 mu g insect(-1) for compound 1, and 122.3 and 381.0 mu g insect(-1) for compound 2. The potential value of extracts and amides derived from P. tuberculatum as efficient insecticides against velvetbean caterpillars is discussed. (c) 2007 Society of Chemical Industry.

Effect of the insect growth regulator diflubenzuron on the predator Podisus nigrispinus (Heteroptera: Pentatomidae)

Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) is a common natural predator of defoliating caterpillars in agricultural and forest systems. Insecticides acting as growth regulators of insect pests can indirectly affect their predators through consumption of contaminated prey. We examined the reproductive performance of P. nigrispinus fed on caterpillars of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae) reared on soybean leaves exposed to the chitin synthesis inhibitor, diflubenzuron. Caterpillars of A. gemmatalis were fed for 12 h with treated soybean leaves and offered to adults of the predator P. nigrispinus over five consecutive days. The fertility of P. nigrispinus was reduced when feeding on diflubenzuron treated caterpillars, especially at the beginning of the reproductive period, but recovered 3 weeks later. The effects of diflubenzuron ingestion on the life table parameters of P. nigrispinus included an increase in the period taken to double the population size, and reductions in the intrinsic rate of population increase, generation duration, and net reproductive rate. Diflubenzuron therefore had an indirect negative effect on the reproduction and the population dynamics of the non-target predator P. nigrispinus. Clearly, its use in integrated pest management requires further evaluation.

Estudos de dispersão de lagartas em milho e soja visando ao manejo de pragas no Brasil e EUA

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Ultramorphology of digestive tract of Anticarsia gemmatalis (Hubner, 1818) (Lepidoptera : Noctuidae) at final larval development

The digestive tract of insects is an important natural, physical, and chemical defense barrier against pathogen invasion. Certain. lepidopteran caterpillars are serious pests of agricultural crops and their biology has received much attention, but little is known about the larval noctuid gut. The morphological analysis of the digestive tract in Anticarsia gemmatalis under scanning electron microscopy (SEM) is a good model for studies about its defense mechanism. The material was fixed (2,5% glutaraldehyde solution; 0.1 M-phosphate buffer, pH 7.3), post-fixed (1% osmium tetroxide in the same buffer), dried at critical point, gold coated and analyzed in a SEM 515-Philips. A. gemmatalis digestive tract consists of a straight duct of varying length and diameter, subdivided in three main regions: the foregut formed by the oral cavity, pharynx, esophagus, and crop; the midgut that is the largest portion of the digestive tract without noticeable morphological differentiation along its length; and the hindgut that is morphologically differentiated in pylorus, ileum, colon, and rectum. Although the general morphology of the A. gemmatalis digestive tract is quite similar to the other Lepidoptera species, the anatomical array of the crop muscular layers is quite different comparing with the description for other larval insect.

Potencial do controle biológico para o controle de Pseudoplusia includens (Walker, 1857) e Anticarsia gemmatalis Hubner, 1818 (Lepidoptera: Noctuidae) em soja

The soybean (Glycine max) is of great importance to national economic scenario being a major Brazilian agribusiness products. In most regions, the caterpillar-of-soy (Anticarsia gemmatalis) and caterpillar-false-Medideira (Pseudoplusia includes), act as defoliators, with the highest incidence, usually during the growing season, until the end of flowering, and thus causing a significant reduction in the production, which requires control measures. Due to market demands and the large external environmental awareness exists today, the methods of ecological management have been highlighted in modern agriculture. The use of chemical insecticides, besides being harmful to the environment and man, is, in most cases, the high cost to the farmer. The biological pest control using natural enemies can be used as an alternative control method. Thus this literature review is intended to provide the updated information about these pests and biological control as an alternative form of control, as well as one more tool in the integrated pest of soybean.

Palmistichus elaeisis (Hymenoptera: Eulophidae) parasitizing pupae of Citioica anthonilis (Lepidoptera: Saturniidae) collected on Piptadenia gonoacantha (Fabaceae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Factors Influencing Expression of Antixenosis in Soybean to Anticarsia gemmatalis and Spodoptera frugiperda (Lepidoptera: Noctuidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Associação de bioensaios e caracterização molecular para seleção de novos isolados de Bacillus thuringiensis efetivos contra Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)