Fumonisina B1 em arroz: desenvolvimento de método analítico, validação e ocorrência

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação de fumosina B1, por CLAE: estudo da estabilidade do derivado fumonisina B1-orto-ftaldialdeído

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação de fumonisina B1 por CLAE: estudo da estabilidade do derivado fumonisina B1-orto-ftaldialdeído

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Analysis of DNA damage induced by aflatoxin B1 in Dunkin-Hartley guinea pigs

Aflatoxin B1 (AFB1) is among the most potent naturally occurring carcinogens and classified as a group I carcinogen. Since the ingestion of aflatoxin-contaminated food is associated with several liver diseases, the aim of the present study was to evaluate the effect of 2, 20, and 200 ppb of AFB1 on DNA damage in peripheral blood lymphocytes and liver cells in Dunkin-Hartley guinea pigs. The animals were divided into four groups according to the given diet. After the treatment the lymphocytes and liver cells were isolated and DNA damage determined by Comet assay. The levels of DNA damage in lymphocytes were higher animals treated with 200 ppb of AFB1-enriched diet (P = 0.02). In the liver cells there were a relationship between the levels of DNA damage and the consumption of AFB1 in all studied groups. These results suggest that Comet assay performed on lymphocytes is a valuable genotoxic marker for high levels of exposure to AFB1 in guinea pig. Additionally our results indicate that the exposure to this toxin increases significantly and increases the level of DNA damage in liver cells, which is a key step on liver cancer development. We also suggest that the Comet assay is an useful tool for monitoring the genotoxicity of AFB1 in liver. © 2007 Springer Science+Business Media B.V.

Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein

A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein expressed in E. coli cells. Solubilization of recombinant human interferon β1 ser17 (rhIFN-β1 ser17) was accomplished by employing a brief shift to high alkaline pH in the presence of non-ionic detergent. The recombinant protein was purifi ed by three chromatographic steps. N-terminal amino acid sequencing and mass spectrometry analysis provided experimental evidence for the identity of the recombinant protein. Reverse phase liquid chromatography demonstrated that the content of deamidates and sulphoxides was similar to a commercial standard. Size exclusion chromatography demonstrated the absence of high molecular mass aggregates and dimers. The protocol represents an effi cient and high-yield method to obtain bioactive homogeneous monomeric rhIFN-β1 ser17 protein. It may thus represent an important step towards scaling up for rhIFN-β1 ser17 large-scale production. © 2010 Villela AD, et al.

Desempenho, qualidade de ovos e metabolismo lipídico de poedeiras comerciais alimentadas com dietas contendo aflatoxina, fumonisina e adsorvente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de imunossensores para aflatoxina B1

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adsorvente de micotoxinas em dietas de frangos de corte contendo aflatoxina e fumonisina

Pós-graduação em Ciência e Tecnologia Animal - FEIS

O campo árido dos fraseologismos. (QUALIS B1)

In the field of phraseological studies, it is clear that there are no strict limits able to establish accurately the various types of word combinations. There is not even a general agreement on what units are objects of study of phraseology, nor what names they should receive. Therefore, this paper aims to raise some issues related to the different terminology used by researchers in this field, which far from providing solutions to the controversial question of phraseologisms, show that terminological profusion can not only hinder scientific advancements in this field, but also indicate that much remains to be done in the area of phraseological studies.

Estudo da influência do agente nucleante )ALTi5%B1%) na microestrutura de chapas AA8011 obtidas por lingotamento contínuo

The aluminum includes several properties with excellent relation between weight and mechanical resistance. With technological advances, increasingly demand the development of new alloys and other production processes in order to reduce the cost of production and insert these new alloys in broader applications. The process of continuous caster (TRC promoted the unite of the aluminum smelting process with the first stage of rolling, making it most economical through the merger these two phases besides transform the continuous casting process. The AA8xxx series is one of the most versatile aluminum alloys and the most often used in continuous caster process provided a great potential application in the market. In order to further, optimize the process it is necessary to increase awareness of the aluminum solidification phenomena associated with the addition of grain refiner, and control of some aluminum production parameters in the process (production rate, metal temperature, etc.). In this study, AA8011 alloy samples were taken in the raw state obtained by the continuous casting process. The samples were laminated to a thickness of 7mm during the process itself and analyzed at three points along its width by microstructural analysis throughout its thickness, the variation rate of addition of the grain refiner in order to assess the influence of this addition with crystallographic formation and some formation of intermetallic precipitates during the solidification. Through this work, it was possible to improve the knowledge related to the addition of refiner with the monitoring of these production processes

Measurement of the production cross section ratio sigma(chi b2(1P))/sigma(chi b1(1P)) in pp collisions at √s=8TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Photomorphogenic modulation of water stress in tomato (Solanum lycopersicum L.): the role of phytochromes A, B1, and B2

Phytochromes are red/far-red light photoreceptors that mediate a variety of photomorphogenic processes in plants, from germination to flowering. In addition, there is evidence that phytochromes are also part of the stress signalling response, especially in response to water deficit stress, which is the major abiotic factor limiting plant growth and crop productivity worldwide. In this study, we used the phyA (far red-insensitive; fri), phyB1 (temporary red-insensitive; tri) and phyB2 mutants of tomato (Solanum lycopersicum L.) to study the roles of these three phytochromes in drought stress responses. Compared to wild type (WT) plants grown under water-deficit stress conditions, the fri, tri, and phyB2 mutants did not exhibit altered dry weights, leaf areas, stomatal densities, or stomatal opening. The stomatal conductance of all three mutants was severely reduced under both fully-hydrated and water-deficit conditions. Although relative water contents did change after drought stress in each mutant, the most significant reduction in water potential during water stress was observed in the fri mutant. However, this mutant returned its water status to WT levels during rehydration. Although the phyB2 mutant lost more water from detached leaves during abscisic acid (ABA) treatment, phyB2 behaved like WT plants, indicating that this mutant was not insensitive to ABA. Overall, these results indicate that the phytochromes phyA, phyB1, and phyB2 modulate drought stress responses in tomato.

Desempenho de um trator agrícola equipado com pneus radiais e diagonais com três níveis de lastros líquidos

A utilização correta de pneus em tratores, tanto em relação ao seu tipo quanto à calibração de sua pressão interna, e a lastragem ideal para cada condição de carga são fatores que influem significativamente no desempenho do trator. Esta pesquisa teve como objetivo comparar o desempenho de um trator equipado com pneus radiais e com pneus diagonais, para três condições de lastragem líquida (0%, 40% e 75% de água), em três condições superficiais de um Nitossolo Vermelho distrófico (superfície firme, preparada e com cobertura vegetal de resto de milho) e em três velocidades teóricas de deslocamento (4 km h-1, 5 km h-1 e 7 km h-1), informadas no painel do trator, correspondendo às marchas B1, B2 e C1. O melhor desempenho do trator, equipado com pneu diagonal, ocorreu na condição de 75% de água nos pneus, apresentando maior velocidade de deslocamento, menor patinhagem do trator, menor consumo horário de combustível e gerando maior potência na barra de tração. Com pneus radiais, o melhor desempenho do trator ocorreu na condição de 40% de água nos pneus, proporcionando maiores velocidades de deslocamento do trator, menores patinhagens, menores consumos, horário e específico de combustível, e maiores potência e rendimento na barra de tração.

Infection of commercial laying hens with Salmonella Gallinarum: clinical, anatomopathological and haematological studies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Newcastle disease in white Pekin ducks: response to experimental vaccination and challenge

A total of 120 Pekin ducks were distributed at random into four experimental groups, vaccinated or not against Newcastle disease (ND): G1 (Ulster 2C strain), G2 (B1 strain), G3 (LaSota strain), and G4 (nonvaccinated group). At 60 days of age, all groups were challenged with a pathogenic ND virus (NDV) suspension, and a group of specific pathogen free (SPF) chicks (G5) was also inoculated. Cloacal and tracheal swabs from all birds were collected after six, 14, 20, and 30 days post-challenge for virus isolation. NDV was isolated in 100% of SPF chicks. Pekin ducks from all groups, vaccinated or not, did not show any ND clinical signs, demonstrating that these birds are not susceptible to ND clinical disease. In the control group (G4), the virus was isolated 20 to 30 days after challenge, suggesting their possible NDV carrier state. In the vaccinated groups, no virus was isolated. This demonstrates that vaccination of white Pekin ducks against NDV is important to reduce NDV shedding in the field.