Variability of the conserved V3 loop tip motif in HIV-1 subtype B isolates collected from Brazilian and French patients

The diversity of the V3 loop tip motif sequences of HIV-1 subtype B was analyzed in patients from Botucatu (Brazil) and Montpellier (France). Overall, 37 tetrameric tip motifs were identified, 28 and 17 of them being recognized in Brazilian and French patients, respectively. The GPGR (P) motif was predominant in French but not in Brazilian patients (53.5% vs 31.0%), whereas the GWGR (W) motif was frequent in Brazilian patients (23.0%) and absent in French patients. Three tip motif groups were considered: P, W, and non-P non-W groups. The distribution of HIV-1 isolates into the three groups was significantly different between isolates from Botucatu and from Montpellier (P < 0.001). A higher proportion of CXCR4-using HIV-1 (X4 variants) was observed in the non-P non-W group as compared with the P group (37.5% vs 19.1%), and no X4 variant was identified in the W group (P < 0.001). The higher proportion of X4 variants in the non-P non-W group was essentially observed among the patients from Montpellier, who have been infected with HIV-1 for a longer period of time than those from Botucatu. Among patients from Montpellier, CD4+ cell counts were lower in patients belonging to the non-P non-W group than in those belonging to the P group (24 cells/µL vs 197 cells/µL; P = 0.005). Taken together, the results suggest that variability of the V3 loop tip motif may be related to HIV-1 coreceptor usage and to disease progression. However, as analyzed by a bioinformatic method, the substitution of the V3 loop tip motif of the subtype B consensus sequence with the different tip motifs identified in the present study was not sufficient to induce a change in HIV-1 coreceptor usage.

Laboratory essays on solid residues from an area of sand mining activities at Analandia, São Paulo, Brazil, and implications to the water resources quality

This investigation reports the results of tests performed in a laboratory with solid waste samples from an area belonging to Sibelco Mineracao Ltd., which is located around Analandia municipality, nearly in the center of São Paulo State, Brazil. Dissolution and leaching essays were realized under different experimental conditions in four samples collected from the mining front and decantation pool, with the aim of evaluating the possibility of release of several constituents to the liquid phase.

Viagens ao 'outro lado': o percurso poético lecléziano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A tradição literária poética e sensorial em Le Clézio

A segunda metade do século XIX mostra-se como um período no qual em cada um dos grandes poetas se abre e se fecha o leque de correspondências da analogia: Baudelaire, Verlaine, Rimbaud, Mallarmé, expressam em sua obra essa tendência à busca da verdadeira vida, do real oculto, movimento em que as sensações desempenham um papel primordial. Atualmente, a obra de Le Clézio vem confi rmar a procura de um mundo completo suscitado pela poesia, o único modo possível de se traduzir em palavras o êxtase sensorial: é o que mostram tanto seus ensaios, quanto suas obras fi ccionais, de L’extase matérielle e L’Inconnu sur la terre a Mondo et autres histoires, Désert, La Quarantaine e Coeur brûle et autres romances, corpus selecionado para a discussão do assunto.

A natureza participante de Eisenstein / Eisenstein’s participant nature

Este artigo analisa alguns ensaios de Eisenstein escritos entre 1945 e 1947, que se encontram na antologia francesa intitulada La non-indifférente nature, de 1976. Fio condutor dos escritos é uma indagação sobre a natureza orgânica da obra de arte, encontrada nas mais variadas manifestações artísticas: em cinema, teatro, literatura, pintura, arquitetura, música. A “organicidade” das obras artísticas decorria do fato de elas serem construídas conforme os elementos estruturais do fenômeno representado: a ligação entre realidade e obra não consistiria numa reprodução de conteúdos, mas na recriação, em todos os níveis formais da obra, da lei dialética que rege a realidade. This paper analyses some essays by Eisenstein written between 1945 and 1947, included in a French anthology of 1976 entiled La non-différente in nature. The guideline of such writings is a quest for the “organic nature” of the work of art, found in the most varied artistic manifestations: cinema, drama, literature, painting, architecture, music. The “organicity” of works of art derived from the that they were built according to the structural elements of the phenomenon represented: the connection between reality and work would not consist of a duplication of contents, but in the recreation, in all the formal levels of the work, of the dialectic law which rules reality.

Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)