Exigências energéticas para mantença e para o crescimento de frangos de corte

O trabalho foi realizado com o objetivo de determinar, pelo método fatorial, as exigências de energia metabolizável (EM) de frangos de corte. Foi conduzido um ensaio em câmaras climáticas mantidas às temperaturas de 13, 23 e 32ºC (±2ºC) para se verificar o efeito da temperatura sobre as exigências de energia metabolizável para mantença utilizando-se a técnica do abate comparativo. As exigências de energia metabolizável para ganho de peso foram determinadas com base no teor de energia corporal e na eficiência energética de utilização da EM. As exigências de mantença foram de 159,36; 116,17 e 128,66 kcal/kg0,75/dia para 13, 23 e 32ºC, respectivamente, verificando-se efeito quadrático da temperatura sobre as exigências de mantença (EMm=300,14 - 14,61.T + 0,2876.T², r²=0,90). As exigências de em para ganho de peso corporal foram de 3,72 kcal/g para machos e 3,98 kcal/g para fêmeas de 1 a 21 dias; 4,21 para machos e 3,93 para fêmeas de 22 a 42 dias; e 4,51 para machos e 7,04 para fêmeas de 43 a 56 dias. Considerando-se as exigências determinadas, foram elaborados modelos de predição das exigências diárias de energia para frangos de corte, nos quais foram considerados o peso corporal, a temperatura ambiente (para estimativa das exigências de mantença) e o ganho de peso (para cálculo das exigências de ganho). Com base nas comparações das exigências determinadas pelos modelos e nas recomendações do manual da linhagem, conclui-se que os modelos elaborados predizem as exigências energéticas dos frangos de corte.

Equações de predição das exigências protéicas para frangos de corte

O objetivo do trabalho foi determinar equações de predição das exigências de proteína bruta (PB) para frangos de corte machos e fêmeas através do método fatorial. Na determinação das exigências de proteína bruta para mantença, foi utilizada a técnica do balanço de nitrogênio. As exigências de proteína bruta para o crescimento foram determinadas em função do conteúdo de nitrogênio na carcaça e a eficiência de utilização do nitrogênio da dieta. A partir dos valores das exigências para mantença e para crescimento foram elaboradas equações de predição para as exigências diárias de PB (g/ ave/ dia) para frangos de corte machos (7 a 21 dias - PB = 1,323xP0,75 + 0,256xG, 22 a 42 dias - PB = 1,323xP0,75 + 0,277xG e 43 a 56 dias - PB = 1,323xP0,75 + 0,283xG) e fêmeas (7 a 21 dias - PB = 1,748xP0,75 + 0,258xG, 22 a 42 dias - PB = 1,748xP0,75 + 0,274xG, e 43 a 56 dias - PB = 1,748xP0,75 + 0,300xG), em que P = peso corporal (kg) e G = ganho de peso diário (g/dia). Recomenda-se a utilização das equações para a determinação das exigências mínimas de nitrogênio ou proteína bruta somente com atenção no atendimento das exigências em aminoácidos.

Crescimento e esporulação de duas espécies de arthrobotrys corda em diferentes meios de cultura e dois ambientes

The researches on the biological control of nematodes with nematophagous fungi has been intensified in recent years. The knowledge of the ecological conditions for the growth and sporulation of these fungi is a prerequisite for attainment of pure cultures needed to attend the demand for formulation of these organisms. With the objective to evaluate the micelial growth and sporulation of Arthrobotrys musiformis and A. oligospora in two environments (B.O.D at 25 +/- 1 degrees C and the environment of the Laboratory), 20 cultures media prepared with common materials found in the communities and industrialized media such as mycological agar, PDA and CMA were evaluated. The media were tested in Petri dishes, being the micelial growth of the fungi evaluated daily, during six days. The measured sporulation at the end of the experiment was done by estimation of the number of conidia/Petri dish. The experiment was carried out in a random design following a factorial arrangement of 20 x 2 x 2, corresponding to 20 media, two fungi and two environments, with five replicates. The variance analysis of the data evidenced significant statistical difference by the F Test, at 1% probability, among media x fungi x environment interaction. Fifty percent of the tested media provided the adequate micelial growth of A. musiformis and there was no statistical difference among them, namely: cassava meal (FM), sweet starch (PD), "corn meal agar" (CMA), oat in fine flakes (AFF), agar-water + dextrose (AA+D), mycological agar (AM), potato dextrose agar (BDA), meal of maize (FMI), flour of wheat (FT) and wheat for kibble (TK). In relation to A. oligospora, 75% of the tested media promoted the maximum growth of the fungus, which are: AFF, AM, FM, PD, CMA, AA+D, BDA, FT, TK, the water from the decoction of rice (AAZ), rice in grains (AZG), triturated rice (AZT), thread flour (FR), oats flour (FA), oats in thick flakes (AFG) and flour of maize (FU). In relation to the sporulation the media that had better role for A. musiformis, in decreasing order, were: FR, TK, AFG, BDA, FA, AFF, AM, FMI, AZT and FM, varying between 1,01 x 10(6) and 1,4 x 10(4) conidia/Petri dish. For the A. oligospora sporulation, the CMA medium provided the maximum level with an estimated average of 5,7 x 10(6) conidia/Petri dish. In the general, the best media for the micelial growth and sporulation of A. musiformis had also been the best for A. oligospora. However, some that had been the best for the A. oligospora did not had been efficient for the micelial growth or the sporulation of A. musiformis, indicating that the isolate of A. musiformis in case is more demanding than that A. oligospora one. The evidences from the study indicate that, in Jaboticabal, São Paulo state, the growth and the sporulation of these fungi do not demand special chambers. Some adaptations of an environment at the laboratory, enough to obliterate the light are sufficient.

Respostas hematológicas em tuvira após anestesia com diferentes concentrações de óleo de cravo

The hematological responses in tuvira (Gymnotus aff. inaequilabiatus) after clove oil anesthesia at different concentrations were evaluated in this study. Fish (n = 25) with average length of 26.8 +/- 2.4 cm and weighing 94.4 +/- 23.6 g were separated in five groups composed with five animals each and submitted individually to respective concentrations of clove oil: 40, 80, 120, 160 and 200 mg L-1. After anesthetic recuperation, the blood samples collection were performed, as well as complete hemogram. The results were submitted to analysis of variance, Tukey test (P<0.05) and Sperman's correlation analysis. There was an increase (P<0.05) on hematocrit, red blood cells count, thrombocytes count and in the values of neutrophils with increasing of clove oil concentrations. In addition, positive correlation (P<0.05) were observed on total leukocytes, as well as negative linear correlation (P<0.05) for mean corpuscular hemoglobin concentration (MCHC). The clove oil anesthesia on tuvira cause hematological changes with an effect dose-dependent. These changes should be considered mainly in future studies designed to establish the reference values of hematologic variables for this specie.

Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chromic oxide as a digestibility marker for dogs - A comparison of methods of analysis

The study reported compared coefficients of apparent digestibility (CAD) obtained by total collection (TC) and by chromic oxide (Cr2O3) determined by visible absorption spectrometry (VIS) and flame atomic absorption spectrophotometry (FAAS). These quantification methods were also investigated to assess their precision. Nineteen adult dogs housed in individual digestibility cages were fed three commercial diets, each test including five or seven animals. The experiment was carried out in a completely randomized design with three among-subjects factors (foods) and three within-subject factors (methods), and the dogs were the experimental unit. Estimated CAD for all dietary components in the three diets by either chromium quantification methods did not differ and showed fair agreement with TC results (P > 0.05). Coefficients of chromic oxide recoveries in feces were 1.06 +/- 0.044 and 1.01 +/- 0.045 for VIS and FAAS, respectively, values significantly different (P < 0.05), indicating better recovery for FAAS. Significant differences (P<0.05) were found in the mean concentration of Cr2O3 for diets and feces (17 +/- 0.6 and 19 +/- 0.8g/kg for VIS and FAAS, respectively). Coefficient of variation for FAAS was 1.69% and 1.77% for VIS, which did not differ significantly (P > 0.05). Pearson's correlation coefficient (r=0.99; P < 0.01) indicated positive and significant association among the results for VIS and FASS, indicating reasonable precision and agreement of chromic oxide contents determined by these methods. Therefore, FAAS and VIS can be used successfully to determine Cr2O3 in dog foods and feces. (c) 2006 Elsevier B.V. All rights reserved.

Efeito do uso de óleos vegetais, associados ou não a inseticida, no controle de bemisia tabaci (gennadius, 1889) e thrips tabaci (lindeman, 1888), em feijoeiro, na época das águas

The objective of this experiments was evaluate the control of Bemisia tabaci and Thrips tabaci, through the use of plant oils associated or not to insecticide in bean plants, besides verifying the consequences on yield. The experiment was carried out in the period of rainy seasons, by utilizing Carioca variety. The statistical design was the randomized blocks, by employing a 6x2 factorial scheme ( plant oil versus insecticide), totalizing 12 treatments and four replications. It was concluded thet application of plant oils without insecticide can be utilized on the control of the B. tabaci biotipo B, since their population and the symptoms of golden mosaic was similar to the treatment plant oils plus insecticide; among the plant oils Agrex (R) plant oil promoted a higher control of white fly. For T. tabaci the application of plant oil associated to insecticide provide with a smaller population index when compared to the treatments without this association. The yield was not affected by the population of B. tabaci biotipo B and T. tabaci when plant oils were utilized, associated or not to the insecticide.

Fatores que afetam a oviposição de Bemisia tabaci (genn.) biótipo B (Hemiptera: Aleyrodidae) em feijoeiro

The objective of this work was to evaluate the oviposition behavior of the whitefly Bemisia tabaci (Genn.) biotype B in relation to plant age, adult density and plant region on common beans plants. The genotype Perola was used to do the test. In free-choice tests 20, 30 and 40 day-old plants were used where evaluated the oviposition preference of the insect. In no-choice test, 30 day-old plants were used to evaluate densities of 50, 100 and 150 adults per plants. No-choice test for plant position (apex, median, base) 40 day-old plants were adopted for liberation of adults. The 30 and 40 day-old plants were preferred for oviposition, the densities of 100 adults per plants ensured the number of eggs enough to discriminate common beans genotypes with different whitefly resistance degrees, and apical region of plant were preferred for whitefly egg laying and therefore more suitable for egg sampling in common bean plants.

Resistência de genótipos de feijão-vagem ao ataque de bruquíneos, em condições de laboratório

The aim of this paper was to determine resistance types of snap beans genotypes under infestation of bruchins Zabrotes subfasciatus (Bohemann, 1833) of Acanthoscelides obtectus (Say, 1831) in a no choice and free choice tests. The treatments were arranged in a completely randomized design with four and five replications, respectively. In no choice test, it was evaluated the total number of viable and unviable eggs, dry weight of the consumed food, percentage of emerged insects, weight of insects, longevity of adults and biological cycle of egg to adult. In a free choice test, the number of attracted insects for each genotype and total number of eggs were evaluated. The genotype ARFVI047 presents oviposition non-preference resistance type to Z. subfasciatus. The genotype ARFVI008 presents resistance type of the antibiosis to Z. subfasciatus. The genotypes ARFVI006, ARFVI008 and ARFVI029 present resistance of non-preference for oviposition type to Z. subfasciatus and the genotype HAV 56 black seed presents resistance of non-preference type for feeding of A. obtectus.

Dietas artificiais incorporadas ou não a colmos triturados de variedades de cana-de-açúcar na biologia de Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

It was studied the incorporation or not of shredded stems of sugarcane varieties to artificial diets and their effect in the biology of Diatraea saccharalis (Fabr., 1794) (Lepidoptera: Crambidae) under laboratory conditions. The experiment was carried out at the Department of Plant Protection of FCAV-UNESP, Jaboticabal, São Paulo, Brazil, in 2007, using diets with different amounts of their components (100, 75, 25 e 25% of concentration of the standard diet). The experiment had a complete randomized design in a 4x3 factorial scheme, using 4 concentrations of diets and three treatments, in a total of 12, with 6 replications. The larval, pupal an total periods and viability were evaluated, as well as the larval weight at 15 days, the pupal weight after 24 hours and the longevity of adults without feeding. The diet with 25% concentration of the components compared to the standard diet had a negative effect in the development of D. saccharlis larvae. Shredded dry stalks incorporated or not to the diet affected only the larval and pupal weight whereas the larval weight was more affected when the resistant (RB83-5486) and susceptible (SP80-3280) varieties due to the use of diet with 50% of its components. The diet with 100% concentration of its components are more favorable to the development of D. saccharalis, with 25% while that is less favorable. The diet with 100% concentration of the components associated with the presence of SP80-3280 (susceptible) stem variety provides greater larval weight. The weights of larvae and pupae of insects are negatively affected when RB83-5486 (resistant) shredded dry stem variety is incorporated into the diet. regarding to larval weight the diet with 50% of the concentration of its components provides better discrimination between genotypes resistant and susceptible to sugarcane borer.

The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos

The mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere- (NT-B) and fibroblast- (NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast-derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 9-16 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9-16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth.

Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)