Pitiose cutânea equina de localização atípica tratada topicamente com solução de anfotericina B e DMSO

Background: Cutaneous lesions by Pythium insidiosum infection are commonly observed in horses, especially in those living at flooded environments. Equine pythiosis is characterized by the development of tumoral masses that are frequently located at distal limbs, ventral abdomen, thorax, breast and face. The lesions are usually granulomatous, serosanguineous and ulcerated, most often destroyed by self-mutilation due to the intense pruritus. The proposed treatment includes surgical excision followed by antifungal drugs administration, which can be done systemically or topically. Amphotericin B and dimethyl sulfoxide (DMSO) in association has been successfully used for cutaneous pythiosis topical treatment due to the DMSO property to carry any substance through plasmatic membranes.Case: The present report concerns a 12-year-old mixed breed gelding presenting with self-mutilation of a tumoral mass located at the left flank. The owners reported that the horse had initially presented a small wound that had evolved to a 20-cm in diameter mass in 4 weeks. Tissue samples were collected, processed and stained by the Gomori's methenamine silver (GMS) method. The histopathological analysis revealed Pythium insidiosum hyphae in a granulomatous tissue, especially located at peripheral region, where kunkers were present. Surgical excision of the mass followed by cauterization was indicated as initial treatment, and due to financial reasons, the owners elected only the topical antifungal therapy to control the fungus infection after surgery. Flunixin meglumine was also administrated for five days aiming the control of pain and inflammation. The wound was cleaned with povidone-iodine solution and rinsed with a solution containing, 50 mg, of amphotericin B in 10 mL of sterile water and 10 mL of DMSO. This procedure was carried Out twice a day. The wound healed fast due to an excellent centripetal epithelialization. and the horse was discharged after 64 days showing only 5% of the initial wound area. The owner reported by telephone the complete healing and hair growth 10 days after discharge.Discussion: Despite the atypical location of the tumoral lesion described at the present report, the history and clinical manifestations, especially the intense pruritus, showed similarity with other characteristic reports of equine cutaneous pythiosis. The diagnosis was confirmed by the histopathological examination showing hyphae structures, as described to be evidences of the presence of Pythium insidiosum in the tissue. The surgical procedure was the first step to provide remission of clinical signs, and one day after surgery the pruritus desapeared. After excision of the granulomatous tissue and cauterization, daily topical administration of amphotericin B associated with DMSO was effective in destroying the infectious agent, as observed by the excellent epithelization. A pink granulation tissue grew up providing an ideal surface for epithelial migration and the healing process progressed quickly. Centripetal epithelialization reduced the wound area until 3% of the initial area in 64 days of treatment, when the remaining wound was found almost completely healed and covered with hair. At the present report, the horse presenting pythiosis was only topically treated. The recommended therapy using amphotericin B and DMSO solution was effective, economically viable and low risk, considering that the systemic antifungal therapy usually suggested is expensive and extremely nephrotoxic. The atypical location of the lesion on the left flank shows that any anatomical region can be affected by the fungus, since the conditions for its development were present.

Macroalgas de riachos da Serra da Prata, leste do Estado do Paraná, Sul do Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Soroprevalência da arterite viral equina em mesorregiões paulistas entre 2007 e 2008

This study investigated the occurrence of equine arteritis virus antibodies using neutralization test in 1,400 horses from Campinas and macro metropolitan paulista mesoregions located at São Paulo State, between 2007 and 2008. Eighty (5.7%) samples showed antibodies to virus (titers from 4 to 4,096). Among the 42 cities, 15 (35.7%) presented at least one seropositive animal to equine arteritis virus. of the 238 farms analyzed 41 showed at least one seropositive animal. The occurrence was higher in sport horses like jumping horses, Quarter horses and was similar for female and male. The rate of seropositive was higher in animals older than 24 months of age. These results suggest the virus circulation among horse population in studied farms, and the need for continuing epidemiological surveillance studies.

Avaliação da eficácia de diferentes protocolos de preparo do Plasma Rico em Plaquetas para uso em Medicina Equina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rabies laboratory diagnosis: peculiar features of samples from equine origin

O diagnóstico laboratorial da raiva é realizado através de métodos de pesquisa do corpúsculo de Negri, imunofluorescência direta e inoculação em camundongos. Na maioria dos casos, quando a amostra é bem coletada, bem conservada e o profissional responsável possui experiência, verifica-se concordância entre as técnicas utilizadas. A Seção de Raiva e Encefalomielite do Instituto Biológico ao comparar a sensibilidade das três técnicas diagnósticas, em 3713 amostras (córtex cerebral, cerebelo e hipocampo) recebidas no período de 1980-1994, sendo 3010 da espécie bovina (983 positivas) e 703 da espécie eqüina (111 positivas), observou que, no caso da raiva eqüina, esta concordância não é mantida. Verificou-se, nesta espécie, que somente em algumas oportunidades foi possível identificar, pelo método histopatológico, o corpúsculo de Negri. em relação à prova de imunofluorescência pode-se afirmar que a mesma detectou uma porcentagem menor de amostras positivas, provenientes da espécie equina, em compração com as da espécie bovina, sendo esta diferença estatisticamente significativa. A prova biológica foi a mais sensível, porém houve uma diferença, também significativa, entre o período de incubação em camundongos das amostras de origem bovina e das de origem eqüina. A presença pouco frequente de corpúsculos de Negri e o período de incubação em camundongos mais prolongado, das amostras de origem eqüina, sugerem que devem ser intensificados os estudos da patogenia da raiva nesta espécie.

Novos registros de Lutzomyia longipalpis (Lutz & Neiva, 1912)(Diptera: Psychodidae) na região Centro-Leste do estado de São Paulo, Brasil

A incidência das leishmanioses tegumentar (LTA) e visceral (LVA) americanas, especialmente essa última, em hospedeiros caninos e humanos, encontra-se em expansão no Estado de São Paulo. Na vigilância epidemiológica dessas endemias, torna-se fundamental o conhecimento da distribuição e ecologia das diferentes espécies de flebotomíneos. Assim, a divulgação de novos registros de seus vetores é fundamental para apontar novas áreas de risco para a transmissão dessas doenças. Neste estudo, realizaram-se capturas de flebotomíneos em ambiente de mata, em diferentes localidades dos municípios de Ipeúna, Itirapina e Analândia, entre agosto e setembro de 2007. Foram capturados 248 flebotomíneos de nove espécies diferentes, em Ipeúna, seis e sete espécimes de duas espécies distintas coletados respectivamente em Itirapina e Analândia. A espécie mais abundante em Ipeúna foi Pintomyia pessoai (37,5%), seguida de P. fischeri (33,06%) e Migonemyia migonei (16,53%). Essas três espécies são consideradas importantes vetores de LTA no território paulista. O registro de Lutzomyia longipalpis pela primeira vez em Ipeúna e Analândia e a confirmação de sua presença em Itirapina indicam risco de essabelecimento da LVA na área e a necessidade de mais estudos locais sobre sua ecologia, sobretudo em relação à ocupação de ambientes antrópicos.

Crescimento de genótipos de sorgo plantados nos sentidos Norte-Sul e Leste-Oeste

O objetivo do presente trabalho foi estudar o crescimento de plantas de seis genótipos de sorgo (Sorghum bicolor (L.) Moench), a saber: dois sacarinos, dois graníferos e dois forrageiros, cultivadas com as linhas de plantio orientadas no sentido Norte-Sul e Leste-Oeste. Instalou-se, em setembro de 1994, o ensaio em área experimental da Faculdade de Ciências Agrárias e Veterinárias (FCAVJ) - UNESP, utilizando delineamento em blocos casualizados, com doze tratamentos e três repetições. As avaliações foram feitas mediante amostragens semanais, determinando-se massa seca acumulada, área foliar e distribuição da massa seca nas diferentes partes da planta. Os materiais sacarinos cresceram mais que os graníferos, e os graníferos tiveram ciclo mais curto que os demais. A distribuição de massa seca caracterizou aptidão de cada genótipo, ou seja, alta eficiência na exportação de material para os colmos nos sacarinos, exportação preferencialmente para os grãos nos graníferos, e exportação para colmos e grãos, evidenciando dupla aptidão, nos forrageiros. O padrão de distribuição de massa seca não foi afetado pela orientação das linhas de plantio.

Erosão hídrica pós-plantio em florestas de eucalipto na região centro-leste de Minas Gerais

O objetivo deste trabalho foi avaliar as perdas de solo e água por erosão hídrica, em parcelas-padrão sob chuva natural, no período pós-plantio, em diferentes sistemas de manejo de florestas de eucalipto. O experimento foi instalado em Latossolo Vermelho-Amarelo, muito argiloso, relevo ondulado. As coletas de dados foram realizadas no período de outubro de 2002 a fevereiro de 2004, em eventos de chuva considerada erosiva. Os sistemas estudados foram: mata nativa; pastagem plantada; eucalipto plantado em nível; eucalipto plantado na direção do declive; eucalipto plantado na direção do declive com queima de restos culturais; e solo descoberto. Entre os sistemas florestais, o eucalipto em nível é o que mais se aproxima da mata nativa, em perdas de solo, indicando assim maior sustentabilidade desse sistema. Os maiores valores de perda de água são encontrados no sistema eucalipto plantado na direção do declive com queima de restos culturais, sugerindo que o fogo aumenta a repelência à água e diminui a taxa de infiltração de água no solo. Todos os sistemas de manejo do eucalipto estudados apresentaram perdas de solo inferiores ao limite de tolerância.

Lutzomyia longipalpis (Diptera, Psychodidae) em Cuesta Basáltica, na bacia hidrográfica do Rio Corumbataí, Região Centro-leste do Estado de São Paulo

A incidência das leishmanioses tegumentar e visceral americanas, em especial esta última (LVA), em hospedeiros caninos e humanos, encontra-se em crescente processo de expansão no Estado de São Paulo. Para a vigilância epidemiológica dessas endemias, torna-se fundamental o conhecimento da distribuição e da ecologia das diferentes espécies da fauna flebotomínea vetoras. Assim, a divulgação de novos encontros de seus vetores, sobretudo da Lutzomyia longipalpis, o principal vetor da LVA, é fundamental para apontar novas áreas de risco para a transmissão dessas doenças. Neste estudo, capturas de flebotomíneos foram realizadas em ambiente domiciliar, peridomiciliar e de mata, em diferentes localidades rurais dos municípios de Ipeúna e Itirapina, entre outubro de 2001 e fevereiro de 2004. Foram utilizadas armadilhas luminosas automáticas do tipo CDC, das 18h às 8h, em 14 noites, resultando 420 horas de exposição. Foram capturados 177 flebotomíneos pertencentes a doze espécies. A espécie mais abundante, Nyssomyia neivai, apontada como a principal vetora de LTA no Estado, contribuiu com 85,4% dos espécimes capturados em Ipeúna. O encontro de Lutzomyia longipalpis em uma caverna em Itirapina, aponta para o risco de estabelecimento da LVA na área e a necessidade de mais estudos locais sobre sua ecologia, sobretudo em relação à ocupação de ambientes antrópicos.

Cauda equina syndrome after spinal tetracaine: Electromyographic evaluation-20 years follow-up

CAUDA equina syndrome (CES) has long been recognized as a rare complication of spinal anesthesia.(1) CES has been described after administration of spinal anesthetics with lidocaine(2) and bupivacaine.(3) In 1991,(4) CES was reported after continuous spinal anesthesia with 1% tetracaine. In 1980, at our university hospital, six adult female patients underwent perineal gynecologic surgery using a spinal anesthetic of 2 ml tetracaine, 1.2%, in 10% glucose. The concentration of the injected tetracaine was unknown by the anesthetists. In all cases, lumbar puncture was performed at the L3-L4 interspace with a disposable spinal needle while the patients were in the sitting position. CES was first diagnosed 72 h or later postoperatively; previous diagnosis was not possible because patients had an indwelling urethral catheter. The diagnosis of CES was confirmed in all patients. During the past year, after institutional approval and informed consent, clinical, magnetic resonance imaging, electromyographic examinations, and conduction studies were performed in three of the above patients. Examinations were not possible on the other three patients because one had recently died, another could not be located, and the third refused to participate. T1 and T2 magnetic resonance image readings were obtained with Gadolinium contrast from a 0.5 Tesla General Electric apparatus (General Electric, Tokyo, Japan). Bilateral sensory and motor conduction studies of the sciatic nerve branches were obtained using a two-channel Nihon-Kohden Neuropack 2 (Nihom-Kohden Corporation, Tokyo, Japan). Electromyography was performed in accordance with conventional techniques.(5,6)

Argilominerais de Coberturas Sedimentares Cenozóicas do Centro-leste do Estado de São Paulo

Clay mineral analysis contributed to the mineralogical characterization and stratigraphic setting of continental Cenozoic deposits of central-eastern portion of São Paulo State, southeastern Brazil. These deposits, often thin and discontinuous, comprise the Itaqueri Formation (Cretaceous-Paleogene), Rio Claro Formation (Pliocene) and associated Neocenozoic deposits. X-ray diffractometry provided mineralogical and semiquantitative constitution of clays, while scanning electron microscopy (SEM) provided details about mineralogical constitution, packing of microscopic grains and nature (detrital or diagenetic) of clays. These results supported interpretations about paleoenvironmental conditions during sedimentation, revealing possible links between changing climates (dry or humid) and correlative Cenozoic deposits in southeastern Brazil. In addition, SEM images showed microstructures which influence on geotechnical behaviour (erosion and collapse phenomena) of sedimentary materials.

Modelagem gravimétrica da Bacia de Taubaté-vale do Rio Paraíba do Sul, leste do estado de São Paulo

This paper presents the gravity survey refinement of the Paraíba do Sul river valley, east region of São Paulo state, by acquisition of 646 gravity stations. Further than the traditional Free-Air and Bouguer corrections, it was necessary to apply the Terrain Correction due the proximity of the Mantiqueira and Mar mountain ranges. An enhanced understanding of Taubaté Basin structural framework was the main objetive of this work. As result, we present the Bouguer Gravity Map of the east region of São Paulo State and the Residual Bouguer Gravity Map generated by Upward Continuation and High-pass filtering. The 2D Gravity Modeling was applied in 4 sections, using bi-dimensional models for the bodies that produced the anomalies (Talwani Method). After integrating the gravity data with the interpretation of 11 seismic sections, it was possible to delineate the Taubate Basin structural framework, which shows an utmost sedimentary thickness of 800 m in the depocenters. It was also interpreted an ultramaphic alkaline plug in the subsurface of Caçapava city.

Estratigrafia de seqüências das formações Taciba e Rio Bonito (Membro Triunfo) na região de Mafra/SC, leste da Bacia do Paraná

The Taciba and Rio Bonito (lower Triunfo Mbr) formations are divided into six depositional sequences based on cores, gamma-ray and electrical logs from shallow drillings from northern Santa Catarina State, Each sequence is formed by two systems tracts, a lower one, sandy (lowstand) and an upper one, shaly (highstand). The Taciba Formation has three sequences, S 0 to S 0 sequence S 0 has a thick turbidite sandstone at the base (Rio Segredo Member) that pinches out towards the eastern margin and even disappears in the Mafra outcrop area. Sequence S 1 varies from a thin fluvial-estuarine system to a thick turbidite sandstone of a channelized fan system; S 1 upper shaly system tract is marine in well PP-11, and it is glacially-influenced in well PP-10. Sequence S 2 is a thick sand-stone body of shallow marine origin, but restricted to one well (PP-11); its upper shaly tract is dominated by massive siltstones intercalated with thin, distal tempestites. The lower Triunfo Member (or Taciba-Triunfo transition) begins with the arrival of deltaic clastics of sequence S 3 lower tract, coarsening-up from medial- to proximal delta front sandstones. Sequence S 4 is quite similar to S 3, both showing sand-stone progradation from north to south, as opposed to the southwest-sourced transgressive diamictites. Sequence S 5 consists of fluvial deposits at well PP-12, and two transgressive cycles from wells PP-11 to PP-9, each one of them composed of fluvial-estuarine to marine systems. Well PP-10 is an exception, where the lower cycle presents deglaciation to marine deposits.

Erosão hídrica de Latossolo Vermelho muito argiloso relevo ondulado em área de pós-plantio de eucalipto no Vale do Rio Doce, região Centro Leste do Estado de Minas Gerais

The erosion caused by running water constitutes important cause of environmental degradation and productivity reduction, which justifies its evaluation in areas of eucalypt commercial plantations, mainly in ondulated slope conditions. The present study was intended to evaluate the influence of different management systems, in an undulated eucalypt pos-planting area, upon soil and water losses by erosion. The experiment was installed in a very clayey Red Latosol (Oxisol) under three eucalypt management systems (downhill planting with burning of crop residues - EDq; downhill planting with maintenance of crop residues on soil surface - ED; level planting with maintenance of crop residues on soil surface - EN), native forest (FN), bare soil (SD), and native pasture (PN). The soil and water losses evaluations were performed in standard plots installed in the field. All eucalypt systems presented very low values of soil losses in relation to the established tolerance limit, indicating the adequacy of these management systems concerning water erosion. The sustainability of these environments regarding erosion is indicated by the fact that soil losses in eucalipt were very close to those observed in native forest (reference).