Infrared laser photobiomodulation (lambda 830 nm) on bone tissue around dental implants: A Raman spectroscopy and scanning electronic microscopy study in rabbits

Objective: the aim of this study was to assess, through Raman spectroscopy, the incorporation of calcium hydroxyapatite (CHA; similar to 960 cm(-1)), and scanning electron microscopy (SEM), the bone quality on the healing bone around dental implants after laser photobiomodulation ( lambda 830 nm). Background Data: Laser photobiomodulation has been successfully used to improve bone quality around dental implants, allowing early wearing of prostheses. Methods: Fourteen rabbits received a titanium implant on the tibia; eight of them were irradiated with lambda 830 nm laser ( seven sessions at 48-h intervals, 21.5 J/cm(2) per point, 10 mW, phi similar to 0.0028 cm(2), 86 J per session), and six acted as control. The animals were sacrificed 15, 30, and 45 days after surgery. Specimens were routinely prepared for Raman spectroscopy and SEM. Eight readings were taken on the bone around the implant. Results: the results showed significant differences on the concentration of CHA on irradiated and control specimens at both 30 and 45 days after surgery ( p < 0.001). Conclusion: It is concluded that infrared laser photobiomodulation does improve bone healing, and this may be safely assessed by Raman spectroscopy or SEM.

Sharpening of periodontal instruments with different sharpening stones and its influence upon root debridement--scanning electronic microscopy assessment

The objective of this study was to evaluate, through scanning electronic microscopy, the effect of sharpening with different sharpening stones on the cutting angle of periodontal curettes (Gracey 5-6), and the influence on root surfaces after debridement and planing. The experimental model consisted of two different phases. In the first, the cutting angles of fifteen stainless steel Gracey 5-6 curettes were analyzed under a scanning electronic microscope after being sharpened with different types of stones. In the second phase, the root surfaces of 25 newly extracted teeth were evaluated with a scanning electronic microscope after being debrided with curettes sharpened with different stones. Analysis of the results showed that the synthetic stones (aluminum oxide and carborundum) are more abrasive and produce more irregular cutting angles, whereas Arkansas stones are less abrasive and produce smoother and more defined cutting angles. There was no significant statistical differences among the five groups tested with regard to the degree of irregularity of the root surfaces after instrumentation.

A scanning electronic microscopy study of the action of different desensitizing agents on bovine dentin

This study sought to use scanning electronic microscopy (SEM) to evaluate the dentinal tubule occlusion potential of different desensitizing agents. Ten slices of bovine dentin were divided into six fragments, cleaned (using ultrasound), and etched for 15 seconds with a 35% phosphoric acid solution. All but one of the groups received a different desensitizing agent; the sixth group served as a control and received no additional treatment. After the agents were applied, the dentin specimens were analyzed by SEM and scores were assigned based on the extent of tubular obliteration. Only three agents demonstrated tubular sealing that was significantly different from that of the control group.

Surface structure and electronic properties of Pt-Fe/C nanocatalysts and their relation with catalytic activity for oxygen reduction

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Segmentation of Scanning Electron Microscopy Images From Natural Rubber Samples With Gold Nanoparticles Using Starlet Wavelets

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Improvement of the oral praziquantel anthelmintic effect by cyclodextrin complexation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Checklist das Cryptophyceae do estado de São Paulo, Brasil

Usando como base de informações a monografia sobre o grupo publicada pelo projeto Flora Ficológica do Estado de São Paulo (Programa BIOTA), o acervo depositado no Herbário Científico do Estado de São Paulo Maria Eneyda P. Kauffmann Fidalgo (SP), a Lista de Espécies da Flora do Brasil e artigos científicos, dissertações e teses (com descrições e ilustrações) temos: nove gêneros e 39 espécies de criptoficeas, sendo 16 espécies exclusivas para o Estado de São Paulo, todas registradas em ambientes de água doce. A carência de especialistas no Estado de São Paulo e no Brasil, além de problemas de estratégia amostral, necessidade de uso da microscopia eletrônica e ausência de estudos de biologia molecular, são fatores que devem ter subestimado o conhecimento taxonômico de Cryptophyceae no Estado.

Membranas biológicas homólogas preservadas em solução alcalina seguida de liofilização, glicerina a 98% e por liofilização para implantação em eqüinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação histomorfométrica e ultra-estrutural da mucosa do cólon menor eqüino submetido a distensão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utilização da microscopia eletrônica de varredura como ferramenta de avaliação da estrutura do tecido de abacate 'quintal' após danos mecânicos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alterações ultraestruturais nas vilosidades do jejuno de equinos após distensão

O objetivo do presente estudo foi analisar as alterações ultraestruturais nas vilosidades do jejuno de seis equinos submetidos à distensão intraluminal com solução salina. A pressão intraluminal foi mantida em 25cm de água durante duas horas. As amostras de mucosa intestinal colhidas às: zero hora; duas horas de distensão; e duas horas e 12h de descompressão foram analisadas por meio de microscopia eletrônica de varredura. Avaliaram-se a área e o perímetro das vilosidades e sua densidade, usando-se um programa computacional de análise de imagens (Image J). A distensão luminal promoveu aumentos da área e do perímetro das vilosidades intestinais. Essa alteração ultraestrutural ocorreu somente 12h após a descompressão e considerou-se que a provável causa seria o edema promovido por aumento da permeabilidade vascular decorrente de um processo de isquemia e reperfusão da mucosa intestinal. Concluiu-se que a distensão intraluminal do jejuno equino promoveu, tardiamente, aumento das dimensões das vilosidades intestinais.

LDPE/EVA Composites for Antimicrobial Properties

Composites with antimicrobial activity are of great interest nowadays and the development of titanium dioxide with these functional properties presents interest in academic and industrial sectors.An approach to develop PE composite containing silver microparticles to have an antimicrobial effect is presented. To obtain such antimicrobial composites, LDPE/EVA were processed with Ag particles on TiO2 particles as inorganic carrier substance. Titanium dioxide nanoparticles (P-25) were covered with silver particles using Turkevich Method or citrate reduction method. The Ag/TiO2 particles were dispersed at concentration of 0,8 wt% and 1% wt% in LDPE/ethylene vinyl acetate copolymer (EVA)-(50% w/w) at the melt state in a Haake torque Rheometer. Silver microparticles were characterized with UV-Vis Spectroscopy. The composites thus prepared were characterized through XRD, Ares Rheometer, Scanning Electronic Microscopy (SEM) and JIS Z 2801 antimicrobial tests to study the effects of the addition of particles on rheological properties, morphological behavior and antimicrobial properties. The results showed that incorporation of silver/titanium dioxide particles on composites obtained systems with differents dispersions. The Ag/TiO2 particles showed uniform distribution of Ag on TiO2 particles as observed by SEM-EDX and antimicrobial tests according to JIS Z 2801 shows excellent antimicrobial properties.

Estudos morfoanatômicos da semente e da plântula de espécies de Anileiras (Indigofera L., Leguminosae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo de ovos de quatro espécies do gênero Meccus (Hemiptera, Reduviidae, Triatominae), vetores da doença de Chagas

The objective of this work was to analyze the morphological, morphometrical, and histological characteristics of eggs of four triatomine bugs species still not studied, in order to understand phylogenetic aspects and to facilitate parameters used in taxonomy, with the purpose of a specific and generic characterization of these vectors in public health. The eggs of each species had come from the collections of the Laboratory of Triatomine bugs and Culicid mosquitoes of the Faculdade de Saúde Pública - USP (Faculty of Public Health/USP). The morphologic studies were carried out through optic microscopy (OM) and scanning electronic microscopy (SEM). The eggs were measured with the help of the digital paquimeter Starrett® 727. In relation to histological measures, Microtome Leica RM 2145® was used. Similarities were evidenced in the exocorial architecture of the eggs when they were studied by OM and SEM. This similarity among the eggs suggests a recent speciation, probably derived from common ancestry, representing a monophyletic group. Some structures were also detected by the histological cuts. The study has contributed to the magnification and recognition in generic and specific terms of the Triatominae subfamily. These new data will be able to subsidize a better understanding to determine roles for each vector species and to facilitate parameters to be used in taxonomy.

PREPARATION and CHARACTERIZATION of CELLULOSE/HYDROUS NIOBIUM PHOSPHATE HYBRID

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)