Geração de blastocisto quimérico pela agregação de trofectoderme com a massa celular interna ou com células iPS

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Estágio supervisionado: prática simbólica e experiência inaugural da docência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

São Paulo 2012: Um sonho, um projeto, uma visão

Millennia ago, through the myths of greeks gods and demi-gods, comes a series of celebrations called Pan-Hellenic Games. Among these festivals, the most famous was certainly the Olympic Games - the Olympics. Forbidden by Rome in the third century AD, they are considered to worship the pagan gods, were rescued in 1894 by Pierre de Coubertin. Over 113 years and 29 Olympiads, the event took gigantic proportions and the right to host the Games has become one of the closest racing of the modern world. Catalyst for urban development, the Olympic Games were the subject of dream for the city of Sao Paulo in the 2000’s, and an internal dispute was fought with Rio de Janeiro to get the right to host the 2012 Games. A team of Paulo Mendes da Rocha and associates was responsible for designing the plan. Eliminated from contention, Sao Paulo forgot the Olympic dream, and in October 2009 the city of Rio was finally chosen to host the Olympics in 2016. This project aims to rescue the look of architectural and urban plan Sao Paulo 2012 and with a critical and poetic eye create a visual identity for a fictitious victory in the election of São Paulo in 2003. Not aiming to create a corporate identity program - because this is not the mission of the architect - but look at Sao Paulo and the Olympics with poetry and criticism in the true Olympic spirit of peace and hope.

Effect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determination

Different cell cycle synchronization methods were used to increase the mitotic index and accuracy of sex determination in murine and bovine embryos. For sexing purposes, colchicine treatment for 2, 4, 6 and 8 h and the FdU-thymidine-colchicine combination were tested in murine embryos. The best results were obtained with colchicine treatment for 8 h (96.88% accuracy) and with FdU-thymidine-colchicine (97.22% accuracy). Mitotic indexes differed significantly between the 2 treatments (21.71% for colchicine and 32.95% for FdU-thymidine-colchicine). For sex identification of murine and bovine demi-embryos, both treatments were demonstrated to be equally effective (nearly 90%). The mitotic index for the FdU-treated murine demi-embryos (19.04%) was higher than the one obtained for the 8-h colchicine treatment (15.62%).