Growth regulator losses from cotton plants due to rainfall

Reguladores de crescimento aplicados às plantas de algodoeiro (Gossypium hirsutum L.) podem ser lavados em função da ocorrência de chuvas. Chuvas que ocorrem próximas à época de aplicação podem ocasionar elevada perda e necessidade de reaplicação dos produtos visando à taxa de crescimento desejada. Avaliou-se o efeito do intervalo de tempo entre a ocorrência de chuva simulada e a aplicação de cloreto de mepiquat e cloreto de chlormequat no algodoeiro no crescimento das plantas, além de estimar a necessidade de reaplicação dos reguladores. Plantas de algodão foram cultivadas em vasos de 12 L que permaneceram em casa de vegetação. Os reguladores de crescimento foram aplicados 40 dias após a emergência, quando 50% das plantas apresentavam botão floral. A chuva foi simulada 1, 2, 4, 6 e 24 horas após a aplicação dos reguladores. Determinou-se a altura das plantas antes da aplicação dos produtos e a cada 3 dias até o 30º dia. Na colheita, foi avaliado o número de ramos e estruturas reprodutivas, com posterior determinação da massa da matéria seca. Também foi determinado o crescimento acumulado e taxa de crescimento das plantas. Os dois reguladores reduziram a massa da matéria seca das plantas, independente do intervalo para ocorrência da chuva. O crescimento excessivo das plantas foi controlado, porém, com eficiência reduzida quanto menor o intervalo para simulação de chuva. em todos os períodos avaliados houve perda de produtos, com necessidade de reaplicação. A taxa de reaplicação de cloreto de mepiquat para os diferentes intervalos de chuva foi, em média, 17% maior.

The feeding preferences of Spodoptera frugiperda (J. E. SMITH) (Lepidoptera: Noctuidae) on cotton plant varieties

Avaliou-se atratividade e não-preferência alimentar de lagartas recém-eclodidas de Spodoptera frugiperda por partes de plantas e plantas de variedades de algodoeiro. Testes foram realizados a 27 ± 1ºC, UR de 70% ± 10% e fotofase de 14h. Folhas, brácteas, botões florais e cascas de maçãs da variedade BRS Itamarati-90 e folhas de Fibermax-966, Fibermax-977, DeltaOpal, DeltaPenta, BRS Acala-90, Coodetec-408, Coodetec-409, Coodetec-410, BRS-Cedro, BRS-Ipê, BRS-Aroeira, IPR-96, IPR-120, BRS-Araçá, IAC-24 e BRS Itamarati-90 foram utilizadas nos testes de atratividade e não-preferência para alimentação, com e sem chance de escolha. Utilizaram-se 20 lagartas de S. frugiperda por placa de Petri (sistema de arena) por teste, com 10 repetições. Contaram-se lagartas para avaliar atratividade por 60 min e não-preferência para alimentação por 24 h. Folha foi mais atrativa e preferida para alimentação por lagartas de S. frugiperda. em livre escolha, Coodetec-410 foi mais atrativa e BRS Acala-90, Fibermax-966 e DeltaPenta, as de menor atratividade à S. frugiperda; BRS-Araçá, mais preferida para alimentação e BRS-Cedro, BRS Itamarati 90, DeltaPenta, Coodetec-408 e BRS-Aroeira, menos preferidas. Considera-se 46 min., tempo mais adequado para avaliar atratividade de algodoeiro a lagartas de S. frugiperda.

Detached leaf culture: Viability to evaluate 2,4-D toxicity symptoms in cotton apex leaves

To study the viability of detached leaf culture technique, studies were carried out with detached leaves from cotton apex (true trilobed leaves). The prepared leaves were sprayed with 2,4-D amine and ester, at rates of 10, 30, 70, and 100% of the recommended doses. Detached leaves without herbicide spray were used as controls. Simultaneously, a greenhouse experiment was conducted with the same treatments as used for the detached leaves experiment. Toxicity was measured through a 0-to-5 grading according to the percentage of affected leaf area in the detached leaves experiment or examining the affected rate of whole plant as indicated in the greenhouse. Results showed that the ester form of the herbicide induced earlier and more severe toxicity symptoms in detached leaves and greenhouse grown plants. Positive and significant correlations (p < 0.001) were found between toxicity results obtained at 7 and 14 days after application in detached leaves and greenhouse plants (r = 0.97 and 0.92, respectively). Negative, significant correlations (p < 0.005) were found between the toxicity levels found at 7 and 14 days after application in detached leaves and dry matter of cotton plants grown in the greenhouse (r = -0.92 and -0.92, respectively).

Cotton response to mepiquat chloride and temperature

Gibberellin inhibitor growth regulators are used for cotton (Gossypium hirsutum L.) canopy manipulation to avoid excess growth and yield losses. However, under temperatures below or over the optimum for cotton production the effect of mepiquat chloride (MC) has not always been significant. In this experiment, cotton plants were grown in growth chambers to study the response to MC as affected by temperature and to determine if an increase in dose could overcome the temperature effects. Mepiquat chloride was applied at rates of 0, 15 and 30 g ai ha-1 at the pinhead square stage. Plants were then grown under three temperature regimes: 25/15 °C, 32/22 °C, and 39/29 °C (day/night temperatures) for 51 days. Higher temperatures increased plant height, reproductive branches, fruit number, fruit abscission, and photosynthesis per unit area, but decreased leaf area and chlorophyll. The largest effect of MC on plant height was observed when the daily temperature was 32 °C, with nights of 22 °C, which was also best for plant growth. High temperatures not only decreased the effectiveness of MC on plant height control, but also caused lower dry matter and fruit number per plant. Low temperatures (25/15 ºC) decreased cotton growth and fruit retention, but a higher concentration of MC was required per unit of growth reduction as compared with 32/22 ºC. At high temperatures, the rate of MC to be applied must be disproportionately increased, because either plant growth is impaired by high temperature lessening the effect of MC, or degradation of MC within the plant is too rapid.

Potassium supply to cotton roots as affected by potassium fertilization and liming

O algodoeiro (Gossypium hirsutum ) apresenta alta exigência de K e é muito sensível a baixo pH do solo. A maior parte do K chega às raízes das plantas por difusão no solo. Por existir interação do K com Ca e Mg, a calagem pode interferir no movimento do K no solo, afetando a nutrição da planta. O objetivo deste trabalho foi estudar o efeito de calcário dolomítico e de 0, 15, 30, 45 e 60 g kg-1 de K no suprimento de potássio às raízes do algodoeiro. As plantas foram cultivadas por 40 dias em vasos de 5 L contendo um Latossolo Vermelho-Escuro (68% de areia e 16% de argila). Houve um acréscimo na produção de matéria seca e na acumulação de K em função da adubação potássica. A intercepção radicular do K do solo foi também aumentada pela aplicação de K, mas não foi afetada pela calagem. O fluxo de massa e a difusão foram aumentadas linearmente com a aplicação de K até 60 mg kg-1, nos vasos com calagem. em vasos sem calagem a quantidade de K atingindo as raízes por difusão aumentou até 45 mg kg-1, decrescendo com a dose máxima de potássio. do mesmo modo, mais K entrou em contato com as raízes por fluxo de massa com a maior dose do nutriente. Isto aconteceu porque havia mais raízes finas nos vasos sem calagem e com a dose máxima de potássio. Com a diminuição da distância média entre as raízes, houve maior competição entre elas, culminando com a diminuição do K difundido até as raízes do algodoeiro.

Cotton development and yield according to nitrogen application and cover crops

Nitrogen application on the cover and the maintenance of straw, conducted by direct seeding, should meet the needs of agriculture and promote soil conservation. This study evaluates the effect of pre-sowing nitrogen application in cotton crops and cover crops by direct seeding, on the development and yield of cotton. It was conducted in the municipality of Selviria-MS during the agricultural years 2005/06, 2006/07 and 2007/08. The experimental design used was a randomized block design consisting of three cover crops (forage turnip, black oat and white oat) and four nitrogen doses (0, 30, 60 and 90 kg of N ha(-1)) in pre-sowing of cotton. In April 2006, April 2007 and April 2008, the assessments of plant development and also harvesting of the experimental plots of cotton cultivars were conducted. The results showed that after planting the cover crops, the yield and development and the heights of cotton plants increased with the use of pre-sowing N of 90 kg ha(-1), also showing that the forage turnip is a coverage plant that provides increased cotton.

Rainfall intensity and Mepiquat Chloride persistence in cotton

Em parte das regiões onde se encontram as maiores áreas de algodão no Brasil atualmente, o índice pluviométrico está ao redor de 2.000 mm anuais, existindo risco de ocorrer lavagem do Cloreto de Mepiquat (CM) das folhas do algodoeiro antes de ser absorvido pelas plantas. O objetivo deste trabalho foi avaliar a lavagem do CM aplicado no algodoeiro por diferentes laminas de chuva simulada. Os tratamentos constaram de três doses do regulador à base de cloreto de mepiquat: 0, 15.0 e 30.0 g ha-1 e quatro lâminas de chuva simulada: 5, 10, 20 e 40 mm, mais um tratamento sem chuva. Foram utilizados vasos de 12 litros de capacidade e a cultivar Delta Opal. Os parâmetros avaliados foram: altura de plantas, número de ramos reprodutivos, massa de matéria seca, retenção de estruturas reprodutivas e área foliar. Quanto maior a intensidade de chuva ocorrida após a aplicação do regulador maior foi o comprometimento da ação do produto, que repercutiu em interferência no crescimento das plantas. Chuvas de 5.0 mm, ocorridas 90 minutos após a aplicação do cloreto de mepiquat, já causaram prejuízo na ação do produto no crescimento do algodoeiro, sendo o efeito maior com o aumento da quantidade de chuva simulada.

POTASSIUM ABSORPTION AND PARTITIONING IN COTTON AS AFFECTED BY PERIODS OF POTASSIUM-DEFICIENCY

Cotton (Gossypium hirsutum var. Latifolium) was grown in nutrient media, at two K levels: 58.5 mg/K and 11.7 mg/K. Potassium deficiency (11.7 mg K/g of K) was imposed upon cotton plants at different stages of plant development. A sequence of increasing sensitivity to K deficiency among cotton plant parts was observed: leaves < bolls < roots < stems. When K deficiency symptoms are clearly visible in the leaves, all the other plant parts are already affected. Bolls are a very important component in K partitioning within the cotton plant, but K is required most by the bur itself and is not translocated to seeds or fibers. Cotton could overcome a 30 day deficiency late in the season without significant losses in lint and seed cotton yields.

Cotton growth and boron distribution in the plant as affected by a temporary deficiency of boron

The knowledge of nutrient mobility is an important tool to define the best fertilizer management and diagnosis techniques. Patterns of boron (B) mobility in plants have been reviewed, but there is very little information on B distribution and mobility in cotton. An experiment was conducted to study plant growth and B distribution in cotton when the nutrient was applied in the nutrient solution or to the leaves, and when a temporary deficiency was imposed. Cotton (Gossypium hirsutum, Latifolia, cv. IAC 22) was grown in nutrient solutions where B was omitted or not for 15 days. Boron was applied to young or mature cotton leaves in some of the minus B treatments. Root growth decreased when the plants were transferred to B solutions, but there was a full recovery when B was replaced in the nutrient medium. Boron deficiency, even when temporary, reduced cotton shoot dry matter yields, plant height and flower and fruit set, and these could not be prevented by foliar application of B. Because of decreased dry matter production, leaves of deficient cotton plants actually showed higher B concentrations than non deficient leaves. This would be misleading when a mature leaf is sampled for diagnosis. If there is any B mobility in cotton phloem, it is very low.

Efficacy and total release interval of mating disruption pheromone on the control of pink bollworm - Pectinophora Gossypiella - in cotton under field conditions in Brazil

This experiment was developed in order to evaluation the efficiency of pheromone to control the pink bollworm and the total time of its release in cotton field. The experiment was installed in field conditions, in Chapadao do Sul/SP/Brazil, from January to April, 1998. The treatments consisted of 2 areas, being one of 30ha, where it was applied the pheromone and another of 10ha that was chosen as control area and did not receive pheromone. In the treated area, the laboratory synthesized sex pheromone (PB-Rope) was used thought of dispensers that allowed the slow and gradual release of the active substance. A total of 250 dispenser per hectare were evenly hand distributed in the area. The dispensers were wrapped around the plants. Both areas (treated area and untreated area) were monitored by delta trap. For evaluation of the boll damage, the treatment area was divided into 4 sub-areas. Twenty five green bolls were collected at random from each sub-area at 48 and 65 days after pheromone treatment. Bolls were cracked open by hand, and number of the bolls with symptoms of pink bollworm attacks was recorded. For evaluation of the productivity four areas were demarcated in each treatment, where all fibers and seeds harvested were weighted. Release rate of pheromone from dispenser was evaluated through of the weigh of the dispensers. Were marked and weighed in analytic scale, 20 dispensers contend the pheromone, being placed 10 dispensers under the cotton plants in treated area and other 10 dispensers in an open area. To every 15 days the dispensers were retired and weighed in analytic scale and soon after put back in the field in the same places. The results showed that only one application of mating disrupt pheromone, used in a dosage of 250 dispenser/ha, reached 80% of control for pink bollworm. the release period of pheromone from dispenser, after the application, was 120 days.

Expression of a new Bacillus thuringiensis Cry1Ia gene in Escherichia coli with strong activity against cotton pests

The control of cotton pests may be accomplished using Bacillus thuringiensis Cry proteins. For this purpose, the objective of this work was to evaluate the insecticidal activity of a new Cry1Ia protein against neonatal larvae of Spodoptera frugiperda and Anthonomus grandis. The complete cry1Ia gene, previously obtained by PCR with oligonucleotide primers based on the sequenced gene, was cloned into the vector pET28a(+), introduced into Escherichia coli BL21(DE3) and expressed by induction with IPTG. The expression of the Cry1Ia protein was confirmed with molecular weight of approximately 81 kDa. The results demonstrated the efficiency of the bacterial system for the expression of B. thuringiensis Cry1Ia protein, which was subsequently used in quantitative bioassays against S. frugiperda and A. grandis larvae, resulting in an extremely toxic protein for both species. This characteristic is exceptionally important for obtaining transgenic cotton plants resistant to these pests.

The management of cover plant residues for cotton cropped in a no-tillage system

The cutting of plant residue in no-tillage systems under certain environmental conditions becomes necessary to adequately establish and grow crops. This study aims to assess the effect on the yield of different methods of managing millet plant residue in cotton plantations. The study was conducted during the agricultural years 2006/07 and 2007/08, and the treatments included no-mechanical-treatment tillage and the use of a rotary shredder, crimper-roller, and mechanical disintegrator for millet plants before sowing the cotton. Evaluations were performed for the residue fragmentation, emergence speed, percent of soil cover during the cycle and yield of the cotton crop. The emergence speed was faster in the management with the rotary shredder. In 2006/07, the no-tillage treatment showed a rate of loss for soil cover that was 46 percent greater than the disintegrator treatment. The rotary shredder and the disintegrator yielded greater soil coverage during the cultivation cycle, and the yield was highly correlated with the soil cover at 75 days after emergence. The management of the millet residue affected the cotton plants for the two-year study period.

Absence of detection of xanthomonas axonopodis pv. malvacearum in cotton seeds used in the Northeast Region of Mato Grosso do Sul in a three-based crop season study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of cotton cultivars differing in gossypol content on the quality of Aphis gossypii as prey for two species of Coccinellidae

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cotton yield and fiber quality affected by row spacing and shading at different growth stages

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)