Purification, crystallization and preliminary X-ray diffraction analysis of a class P-III metalloproteinase (BmMP-III) from the venom of Bothrops moojeni

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A new class of neurotoxin from wasp venom slows inactivation of sodium current

The effects of alpha-pompilidotoxin (alpha-PMTX), a new neurotoxin isolated from the venom of a solitary wasp, were studied on the neuromuscular synapses in lobster walking leg and the rat trigeminal ganglion (TG) neurons. Paired intracellular recordings from the presynaptic axon terminals and the innervating lobster leg muscles revealed that alpha-PMTX induced long bursts of action potentials in the presynaptic axon, which resulted in facilitated excitatory and inhibitory synaptic transmission. The action or alpha-PMTX was distinct from that of other known facilitatory presynaptic toxins, including sea anemone toxins and alpha-scorpion toxins, which modify the fast inactivation of Na+ current. We further characterized the action of alpha-PMTX on Na+ channels by whole-cell recordings from rat trigeminal neurons. We found that alpha-PMTX stowed the Na+ channels inactivation process without changing the peak current-voltage relationship or the activation time course of tetrodotoxin (TTX)-sensitive Na+ currents, and that alpha-PMTX had voltage-dependent effects on the rate of recovery from Na+ current inactivation and deactivating tail currents. The results suggest that alpha-PMTX slows or blocks conformational changes required for fast inactivation of the Na+ channels on the extracellular surface. The simple structure of alpha-PMTX, consisting of 13 amino acids, would be advantageous for understanding the functional architecture of Na+ channel protein.

A rescaling of the phase space for Hamiltonian map: Applications on the Kepler map and mappings with diverging angles in the limit of vanishing action

A rescale of the phase space for a family of two-dimensional, nonlinear Hamiltonian mappings was made by using the location of the first invariant Kolmogorov-Arnold-Moser (KAM) curve. Average properties of the phase space are shown to be scaling invariant and with different scaling times. Specific values of the control parameters are used to recover the Kepler map and the mapping that describes a particle in a wave packet for the relativistic motion. The phase space observed shows a large chaotic sea surrounding periodic islands and limited by a set of invariant KAM curves whose position of the first of them depends on the control parameters. The transition from local to global chaos is used to estimate the position of the first invariant KAM curve, leading us to confirm that the chaotic sea is scaling invariant. The different scaling times are shown to be dependent on the initial conditions. The universality classes for the Kepler map and mappings with diverging angles in the limit of vanishing action are defined. © 2013 Published by Elsevier Inc. All rights reserved.