Características clínicas e histopatológicas da placa aural em eqüinos das raças Mangalarga e Quarto de Milha

Placa aural é uma variante da papilomatose eqüina. Foram examinados 306 eqüinos da raça Mangalarga e 275 da raça Quarto de Milha, com o objetivo de comparar a ocorrência da placa aural entre os animais destas raças, e caracterizar os achados clínicos e histopatológicos desta enfermidade. A ocorrência da placa aural foi 57% nos eqüinos da raça Mangalarga e 35% nos eqüinos da raça Quarto de Milha. Clinicamente as lesões consistiram de placas aplainadas, descamativas e hipocrômicas, formadas com freqüência pela coalescência de pequenas pápulas. Os principais achados histopatológicos foram hiperplasia epidérmica e hipomelanose levando à alteração abrupta entre o epitélio normal e o epitélio acometido pela placa aural.

Phylogenetic and structural studies of a novel equine papillomavirus identified from aural plaques

Papillomaviruses (PVs) infect a wide range of animal species and show great genetic diversity. To date, excluding equine sarcoids, only three species of PVs were identified associated with lesions in horses: Equus caballus papillomavirus 1 (EcPV1-cutaneous), EcPV2 (genital) and EcPV3 (aural plaques). In this study, we identified a novel equine PV from aural plaques, which we designated EcPV4. Cutaneous samples from horses with lesions that were microscopically diagnosed as aural plaques were subjected to DNA extraction, amplification and sequencing. Rolling circle amplification and inverse PCR with specific primers confirmed the presence of an approximately 8. kb circular genome. The full-length EcPV4 L1 major capsid protein sequence has 1488 nucleotides (495 amino acids). EcPV4 had a sequence identity of only 53.3%, 60.2% and 51.7% when compared with the published sequences for EcPV1, EcPV2 and EcPV3, respectively. A Bayesian phylogenetic analysis indicated that EcPV4 clusters with EcPV2, but not with EcPV1 and EcPV3. Using the current PV classification system that is based on the nucleotide sequence of L1, we could not define the genus of the newly identified virus. Therefore, a structural analysis of the L1 protein was carried out to aid in this classification because EcPV4 cause lesion similar to the lesion caused by EcPV3. A comparison of the superficial loops demonstrated a distinct amino acid conservation pattern between EcPV4/EcPV2 and EcPV4/EcPV3. These results demonstrate the presence of a new equine PV species and that structural studies could be useful in the classification of PVs. © 2012 Elsevier B.V.

Use of PCR to estimate the prevalence of Equus caballus papillomavirus in aural plaques in horses

Aural plaques occur on the skin of the medial surface of the pinnae of horses. In this study the presence of Equus caballus papillomavirus (EcPV)-3 and -4 DNA was assessed in 45 such plaques using a 'touchdown' PCR. Papillomaviruses (PVs) were detected in 62.3% (28/45) of samples: EcPV-3 and -4 DNA in 8.89% (4/45) and 37.78% (17/45) of samples, respectively, with 15.56% (7/45) of samples exhibiting co-infection. Viral DNA was not detected in 37.78% (17/45) of samples, suggesting the possible existence of other equine PVs. Neither EcPV-3 nor -4 were detected in negative control skin. This study is the first to evaluate the prevalence of these two viruses in equine aural plaques. © 2013 Elsevier Ltd.

The invisible blackness of Harryette Mullen's poetry: writing, miscegenation and what remais to be seen

Este ensaio aborda a poética de Harryette Mullen, poetisa afro-americana cuja obra questiona os limites que moldam as expectativas pela inteligibilidade acessível na literatura afro-americana. Os poemas de Mullen exploram as bordas da inteligibilidade, avançando para além das expectativas por uma forma visível/ Rev. Let., São Paulo, v.52, n.1, p.101-120, jan./jun. 2012. 119 inteligível de linguagem que abarcaria a experiência da negritude. Argumenta-se que a escrita na poesia de Mullen funciona como um processo de miscigenação ao jogar com a ilegibilidade da negritude, para além de uma linha visível de distinção entre o que é ou que deveria ser considerado como parte apropriada da negritude, o que possibilita novas formas de reflexão sobre a poesia como um instrumento politicamente significativo para se repensar o papel da poetisa e do poeta negros no espaço da diáspora negra.

The Invisible Blackness of Harryette Mullen's Poetry: Writing, Miscegenation, and What Remains to Be Seen

This essay addresses the poetics of Harryette Mullen, an awarded African-American female poet whose work questions the boundaries that shape the expectations for accessible intelligibility in African-American literature. Mullen’s poems skirt the edges of intelligibility by going beyond the expectations for a visible/intelligible form of language that would embrace the experience of blackness. I argue that writing in Mullen’s poetry works as process of miscegenation by playing on the illegibility of blackness, beyond a visible line of distinction between what is or should be considered part of blackness itself, which engages new forms of reflection on poetry as a politically meaningful tool for rethinking the role of the black (female) poet within the black diaspora.

Pesquisa do DNA viral de papilomavirus equino em lesões de placa aural

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)