Membranas biológicas homólogas preservadas em solução alcalina seguida de liofilização, glicerina a 98% e por liofilização para implantação em eqüinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Função osteoindutora de fragmentos ósseos conservados em glicerina a 98%: estudo experimental em ratos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cistoplastia experimental em coelhos (Oryctolagus cuniculus) com peritônio bovino conservado em glicerol a 98%

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Allogeneic transplants of rib cartilage preserved in 98% glycerol or 70% alcohol into the malar process of rats: a comparative histological study.

A comparative study was made of two methods of cartilage preservation, 98% glycerol and 70% alcohol. Rib cartilage was treated by either of these methods and transplanted into the malar process of rats. Cartilage grafts preserved by both methods were equally well tolerated. Resorption and bone substitution were similar in both groups after 120 days, although resorption was greater for the alcohol-preserved cartilage up until day 30. The possible reduction in antigenicity by the 98% glycerol did not produce any difference of behavior from the cartilage preserved in 70% alcohol.

Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.

As a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.

Implantação de biomembrana de colágeno tratada em solução alcalina ou conservada em glicerina a 98% na parede abdominal de equinos

The objective of this research was obtain collagen biomembranes treated in alkaline solution for 72 hours (GE) from tendineous diaphragmatic center of equines and compare its biocompatibility with membranes preserved in a glycerin solution 98% (GG) and membranes do not treated (GC). The membranes were implanted in the internal fascia of recto abdominis muscle of equines and removed, with adjacent tissues, seven, 63 and 126 days postoperative for the preparation of histological slides. The histomorphometric study revealed more intense inflammatory process to GG and CC implants and faster healing for GE implants. It was concluded that the collagen biomembranes treated in alkaline solution is more biocompatible than biomembranes preserved in 98% glycerin.

Morfologia e estereologia do peritônio de paca conservado em solução supersaturada de açúcar a 300% ou glicerina a 98% implantados na parede abdominal de ratos

Pós-graduação em Cirurgia Veterinária - FCAV

Regime geral da responsabilidade ambiental e disciplian na Lei n. 9.605/98

Pós-graduação em Direito - FCHS

A adesão do Brasil ao TNP: uma análise da trajetória da questão nuclear brasileira nos governos de Fernando Collor de Mello (1990-92) e Fernando Henrique Cardoso (1995-98)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Propriedades tensiométricas do peritônio da paca (Cuniculus paca) a fresco e conservado em glicerina 98%

In constant searching for alternative biological material to perform implants and new options of experimental animal models, the objective of this investigation was to describe the mechanical properties of the peritoneum paca (Cuniculus paca Linnaeus, 1766) fresh and preserved in 98% glycerin. Samples of fresh and preserved in glycerin for periods of 30, 60 and 90 days were subjected to mechanical tests. Four adult animals, male or female, with mean body weight of eight kilograms, were used for collecting samples of the peritoneum. All tissues preserved in glycerin 98% showed a decrease in stiffness and increase in ductility and toughness. Considering the maximum force applied to the peritoneum, significant increase was observed in values (p<0.01) of samples stored for 60 and 90 days when compared to fresh material. In relation to the stretch variable, an increase was observed in all storage time of glycerin samples, verifying significant difference (p<0.01) when compared with the fresh samples. The variable area also showed significance (p <0.01) between the values of the fresh samples (5.40 mm(2)) and preserved in the glycerin by periods of 30 days (4.50 mm(2)), 60 days (9.00 mm(2)) and 90 days (7.20 mm(2)), thus indicating that the area of this membrane increased by 0.033 mm(2) per day. Generally, it was concluded that the 98% glycerin is a substance effective for the preservation of the peritoneum of the agouti paca, therefore improves its mechanical properties allowing the support membranes greater deformation forces. Thus, the results obtained in mechanical tests of the peritoneum of paca suggest its use as an alternative biological material.

O uso do peritônio de paca conservado em solução supersaturada de açúcar a 300% ou glicerina a 98% implantados na parede abdominal de ratos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)