Canine ehrlichiosis: clinical, hematological, serological and molecular aspects

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Resposta de mudas de goiabeira à aplicação de zinco

O subsolo, normalmente utilizado para produção de mudas de frutíferas, apresenta baixa concentração de zinco e, assim, existe grande probabilidade de resposta à aplicação deste micronutriente. Considerando a falta de informações sobre o assunto, procurou-se avaliar o efeito da aplicação de zinco ao substrato de produção das mudas de goiabeira e acompanhar os efeitos no desenvolvimento, na produção de matéria seca e no estado nutricional das plantas. O delineamento experimental foi em blocos ao acaso, com cinco tratamentos e cinco repetições. As doses de zinco, na forma de sulfato de zinco, foram: 0; 2; 4; 6 e 8 mg dm-3 de Zn. No plantio, as mudas receberam doses de N, P, K e B de 300; 100; 150 e 0,5 mg dm-3, respectivamente. O experimento foi conduzido em viveiro telado, em vasos com 2,8 dm³ de substrato de um Argissolo Vermelho-Amarelo. Após 135 dias do plantio, avaliaram-se: a altura, a área foliar e a matéria seca da parte aérea e das raízes, bem como os teores de macronutrientes e de Zn. As mudas de goiabeira responderam positivamente à aplicação de zinco. O maior desenvolvimento das mudas esteve associado à dose de 2 mg dm-3 de Zn. Doses iguais ou superiores a 4 mg dm-3 causaram redução significativa no desenvolvimento e no acúmulo de macronutrientes nas mudas de goiabeira.

Models for genetic evaluation of Nelore cattle mature body weight

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influence of genetic type and level of concentrate in the finishing diet on carcass and meat quality traits in beef heifers

Carcass and meat quality traits of thirty-six feedlot beef heifers from different genetic groups (GG) fed at two concentrate levels (CL) were evaluated using 12- Nellore (NE), 12 - 1/2Angus x 1/2Nellore (AN) and 12 - 1/2Simmental x 1/2Nellore (SN) animals. Six heifers of each GG were randomly assigned into one of two treatments: concentrate at 0.8% or 1.2% of body weight (BW). Heifers fed concentrate at 0.8% of BW had greater (P<0.05) dressing percentage. None of the proximate analysis components of the beef were affected (P>0.05) by either CL or GG. Heifers from the AN group had higher (P<0.05) carcass weights, 12th rib fat thickness and lower dressing percentage (P<0.05) compared to the other groups. NE heifers had greater WBSF values (P<0.05) than the other genetic groups. Data suggest that the concentrate level can be reduced without compromising meat quality traits. (C) 2011 Elsevier Ltd. All rights reserved.

Estimação de componentes de co-variância para pesos corporais do nascimento aos 365 dias de idade de bovinos Guzerá empregando-se modelos de regressão aleatória

Um total de 19.770 pesos corporais de bovinos Guzerá, do nascimento aos 365 dias de idade, pertencentes ao banco de dados da Associação Brasileira dos Criadores de Zebu (ABCZ) foi analisado com os objetivos de comparar diferentes estruturas de variâncias residuais, considerando 1, 18, 28 e 53 classes residuais e funções de variância de ordens quadrática a quíntica; e estimar funções de co-variância de diferentes ordens para os efeitos genético aditivo direto, genético materno, de ambiente permanente de animal e de mãe e parâmetros genéticos para os pesos corporais usando modelos de regressão aleatória. Os efeitos aleatórios foram modelados por regressões polinomiais em escala de Legendre com ordens variando de linear a quártica. Os modelos foram comparados pelo teste de razão de verossimilhança e pelos critérios de Informação de Akaike e de Informação Bayesiano de Schwarz. O modelo com 18 classes heterogêneas foi o que melhor se ajustou às variâncias residuais, de acordo com os testes estatísticos, porém, o modelo com função de variância de quinta ordem também mostrou-se apropriado. Os valores de herdabilidade direta estimados foram maiores que os encontrados na literatura, variando de 0,04 a 0,53, mas seguiram a mesma tendência dos estimados pelas análises unicaracterísticas. A seleção para peso em qualquer idade melhoraria o peso em todas as idades no intervalo estudado.

Patogenicidade de Verticillium lecanii ao pulgão-do-pinus

Avaliou-se a patogenicidade do isolado IBCB 473 de Verticillium lecanii no pulgão-do-pinus Cinara atlantica (Hemiptera, Aphididae), inseto-praga em plantios de Pinus spp. O delineamento experimental utilizado foi o inteiramente casualizado, com cinco repetições por tratamento e cada repetição constituída de uma placa de Petri (9 cm de diâmetro) contendo 10 ninfas. Foram testadas suspensões de inóculo nas concentrações de 1,0 ' 10(6); 0,5 ' 10(7); 1,0 ' 10(7); 0,5 ' 10(8); e 1,0 ' 10(8) conídios/mL, pulverizando-se 1 mL sobre as ninfas. Como tratamento-testemunha, utilizou-se água esterilizada. As placas de Petri foram mantidas em câmara climatizada a 25 ± 1 °C, 70 ± 10% de UR e fotofase de 12 horas. As avaliações foram realizadas diariamente, anotando-se a mortalidade dos indivíduos de cada tratamento. A dose mais eficiente foi de 1,0 x 10(8) conídios/mL, causando mortalidade de 86,0% após cinco dias da pulverização. Os valores de TL50 das concentrações utilizadas foram de 5,82; 5,24; 4,60; 4,34; e 3,83 dias, respectivamente.

A new chart based on sample variances for monitoring the covariance matrix of multivariate processes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fontes e doses de nitrogênio em cobertura no feijoeiro de inverno irrigado no sistema plantio direto

The common bean is a leguminous of great importance in the Brazilian economy and nitrogen is the taken up nutrient in larger amount. Nitrogen fertilization management is of extreme importance to offer larger economical viability, besides increasing the efficiency of plant in the use of the available resources. The objective this study was to evaluate the effect of sources and doses of sidedressing nitrogen in the development and yield of winter common bean in no tillage system, as well as evaluate its economical viability. A randomized blocks design was used, in a factorial scheme 3x3 with 9 treatments constituted by three sources of nitrogen (ammonium sulphate, urea and ammonium sulphate 1/2 of N + urea 1/2 of N, applied at V(4-3) stadium) and different doses of sidedressing nitrogen (0, 40, 80 kg ha(-1)) in four replications. The study was conducted in Selviria county, MS State in 2004 in no season crop period, in a dystrophic Haplustox soil. The conclusion: independent of nitrogen source, nitrogen fertilization increasing provides increment in yield of winter common bean up to dose 80 kg/ha, and this provides, on average, an increase of 20% in yield compared with control (without sidedressing nitrogen). The urea is the nitrogen source of larger economical efficiency.

Correlação linear entre componentes da produção e produtividade do arroz de terras altas em sistema plantio direto

The rice is one of the main sources of the humanity's feeding. During the agricultural year 2009/2010, in Selviria County, Mato Grosso do Sul State, in the Brazilian Savannah, an experiment was installed with rice upland in a Dystropherric Red Latosol (Typic Acrustox) under no-tillage, irrigated by central pivot, with the purpose of selecting the best components production to explain the variability the irrigated rice yield upland. The geostatistical grid was installed, to collect the data, with 120 sampling points, in an area of 3.0 ha and and homogeneous slope of 0.055 m m(-1). The medium rice yield was of the 5980 kg ha(-1). For the simple lineal regressions, the number of spikelets grenades for panicle presented the best direct potential correlation with the yield rice, given for: PGO = 115,5.NEG(0,770). However, for the multiple lineal regressions, the equation equacao PGO = 2754,30-411,55.NEG-461,07. NEC+436,59. NET it was the one that better she came to esteem the yield rice. However, spatial, it was not possible to establish correlation between the yield rice and the components production, once none of those it presented spatial dependence in their data.

Vascular endothelial growth factor (VEGF) and VEGF-receptor expression in placenta of hyperglycemic pregnant women

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infecção mista pelo Sugarcane mosaic virus e Maize rayado fino virus provoca danos na cultura do milho no estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

V(2)O(5)/TiO(2) catalytic xerogels raman and EPR studies

Raman spectroscopy and Electron Paramagnetic Resonance (EPR) studies were performed on a series of V(2)O(5)/TiO(2) catalysts prepared by a modified sol-gel method in order to identify the vanadium species. Two species of surface vanadium were identified by Raman measurements, monomeric vanadyls and polymeric vanadates. Monomeric vanadyls are characterized by a narrow Raman band at 1030 cm(-1) and polymeric vanadates by two broad bands in the region from 900 to 960 cm(-1) and 770 to 850 cm(-1). The Raman spectra do not exhibit characteristic peaks of crystalline V(2)O(5). These results are in agreement with those of X-ray Diffractometry (XRD) and Fourier Transform Infrared (FT-IR) previously reported (C.B. Rodella et al., J. Sol-Gel Sci. Techn., submitted). At least three families of V(4+) ions were identified by EPR investigations. The analysis of the EPR spectra suggests that isolated V(4+) ions are located in sites with octahedral symmetry substituting for Ti(4+) ions in the rutile structure. Magnetically interacting V(4+) ions are also present as pairs or clusters giving rise to a broad and structureless EPR line. At higher concentration of V(2)O(5), a partial oxidation of V(4+) to V(5+) is apparent from the EPR results.

Histology and ultrastructure of pericardial cells of Scaptotrigona postica latreille (Hymenoptera, Apidae) in workers and queens of different ages

The paper presents a study of the pericardial cells of Scaptotrigona postica an eusocial Brazilian stingless bee. Light and electron microscopy was used in a comparative study on workers and queens of different ages, exerting different functions in the colony. The pericardial cells are found only in the peticardial sinus, mainly in groups around the dorsal vessel. Each cell is enclosed by the basal membrane and its peripheral region is characterized by folds of the plasma membrane, which form canals and loops. The points where the plasma membrane folds is frequently closed by diaphragms, that along with the basal lamina form a barrier to substances from hemolymph. Along the membrane limiting the canals and loops, an intense endocytic activity through coated vesicles takes place indicating a selective absorption of hemolymph components. In older individuals, workers or queens, the cells exhibit larger quantities of cytoplasm inclusions, heterogeneous vacuoles containing the final products of intracellular digestion, and autophagic vacuoles with concentric membranous structures. The pericardial cells general morphology is in accordance with the role in processing metabolites captured from hemolymph and storage of indigested residues. (C) 2006 Elsevier Ltd. All rights reserved.

Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis

The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now has not been developed. The present study focused on the replacement of azocasein with fluorogenic substrates, and on the definition of enzyme specificity. Eight fluorogenic substrates were tested, and the peptide Abz-Ala-Phe-Arg-Ser-Ala-Ala-Gln-EDDnp was found to be the most convenient for use as an operational substrate for ZapA. A single peptide bond (Arg-Ser) was cleaved with a Km of 4.6 µM, a k cat of 1.73 s-1, and a catalytic efficiency of 376 (mM s)-1. Another good substrate for ZapA was peptide 6 (Abz-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-EDDnp) which was cleaved at a single bond (Phe-Ser) with a Km of 13.6 µM, a k cat of 3.96 s-1 and a catalytic efficiency of 291 (mM s)-1. The properties of the amino acids flanking the scissile bonds were also evaluated, and no clear requirement for the amino acid residue at P1 was found, although the enzyme seems to have a preference for a hydrophobic residue at P2.