Aspectos biológicos e técnica de criação do gorgulho-da-goiaba, Conotrachelus psidii marshall (Coleoptera: Curculionidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Occurrence of anti-Neospora caninum antibodies in Brazilian cervids kept in captivity

Neospora caninum is a coccidian parasite that causes disease in captive and domesticated animals and has been found in wild animals such as cervids. Sera from 150 cervids of the genus Mazama, were collected from 31 captive herds and 16 zoos from different Brazilian regions and analyzed by indirect fluorescent antibody test for anti-N. caninum antibodies. Positive reactions were found in 42% (63) of the samples and the titers varied from 50 to 51,200. of the 86 cervids from the captive herds, 38 (44.2%) had anti N. caninum antibodies and of the 64 samples from the zoo, 25 (39.1%) were positive. No significant difference (p > 0.05) was found for the occurrence values observed between the animals from captive herds and zoos as well as within the values documented for each one of the species analyzed. Therefore, the results indicate that the agent is prevalent from cervids in captivity in Brazil. (c) 2005 Elsevier B.V. All rights reserved.

Avaliação do efeito do exercício físico no metabolismo de ratas diabéticas prenhes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aspectos histológicos e morfométricos dos testículos de gatos domésticos (Felis catus)

Nesta pesquisa foram obtidos dados histológicos e morfométricos comparativos sobre os testículos de gatos, pós-orquiectomia, divididos em dois grupos: Grupo 1, gatos com até 1 ano de idade e Grupo 2, animais acima de 1 ano. Verificou-se que: (1) aos 4 meses de idade os túbulos seminíferos apresentaram-se pouco desenvolvidos e com ausência de luz, epitélio seminífero baixo, células de Sertoli indiferenciadas e tecido intersticial escasso; (2) aos 5 meses os túbulos seminíferos começaram a se diferenciar com aumento do diâmetro e luz tubulares e as demais estruturas permaneceram semelhantes à observação anterior; (3) aos 6-7 meses ocorreu o início da espermatogênese e espermiogênese; as células de Leydig apareceram maiores, poliédricas com citoplasma vacuolizado e núcleo claro, e tecido intersticial esparso com poucos vasos sangüíneos; (4) os animais com 1 ano de idade apresentaram morfologia testicular igual à do animal adulto, com túbulos seminíferos de maior diâmetro, epitélio germinativo alto e luz tubular pequena, as células de Leydig aparecendo poliédricas, com dimensões variadas, citoplasma vacuolizado, núcleo claro e nucléolo evidente, e espaço intertubular seminífero variado com vasos sanguíneos, predominantemente evidentes; (5) no Grupo 1 o diâmetro médio dos túbulos seminíferos foi de 160,58µm e no Grupo 2 foi de 185,94µm, sendo os valores médios significantes entre si; (6) a altura média do epitélio seminífero foi de 49,51µm para o Grupo 1 e de 63,29µm para o Grupo 2, estaticamente significantes; (7) os maiores valores mensurados foram obtidos para os gatos do Grupo 2, por serem gatos adultos e portanto com os órgãos reprodutores funcionais.

Effect of dental finishing instruments on the surface roughness of composite resins as elucidated by atomic force microscopy

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Controle biológico da podridão radicular (Pythium aphanidermatum) e promoção de crescimento por Pseudomonas chlororaphis 63-28 e Bacillus subtilis GB03 em alface hidropônica

Podridões radiculares causadas por espécies de Pythium são um importante problema em cultivos hidropônicos. Sintomas de subdesenvolvimento são observados nas plantas parasitadas pelo patógeno, sendo muitas vezes não diagnosticados pelo produtor. O objetivo do trabalho foi avaliar o controle biológico da podridão radicular causada por Pythium aphanidermatum e a promoção de crescimento por Pseudomonas chlororaphis 63-28 e Bacillus subtilis GB03, reconhecidos agentes de controle biológico de doenças de plantas. A inoculação das plantas com P. aphanidermatum ocasionou o subdesenvolvimento, sendo essa diminuição de 20%. A adição dos agentes de biocontrole na solução nutritiva teve um efeito positivo no aumento da massa (6% a 13%), no número de folhas (4% a 7%) e no teor de clorofila (3%) das plantas de alface. Entretanto, maiores estudos devem ser realizados para melhorar a capacidade de controle da doença e de promoção de crescimento pelos agentes de biocontrole estudados no cultivo de alface hidropônica.

Desempenho produtivo de vacas de quatro grupos genéticos submetidas a diferentes manejos alimentares desmamadas aos 42 ou 63 dias pós-parto

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desempenho de bezerros de corte filhos de vacas submetidas a diferentes manejos alimentares, desmamados aos 42 ou 63 dias de idade

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Electron transfer between adjacent layers in self-assembled films

A simple model of electron transfer is adapted to explain fluorescence quenching in self-assembled films of poly( p-phenylene vinylene) (PPV) alternating with poly(thiophene acetic acid) (PTAA). Quenching is caused by a photo-induced electron transfer between the excited PPV (donor, D) and the PTAA (acceptor, A). The electron-transfer process can be mediated by insertion of electronically inert spacing bilayers between the D and A layers, As the number of bilayers is increased, the fluorescence is gradually recovered which is explained theoretically by assuming that the electron-transfer rate can be described as k = Z exp(- beta r) where r is the distance between D and A. (C) 2000 Elsevier B.V. B.V. All rights reserved.

Use of chemically modified silica with beta-diketoamine groups for separation of alpha-lactoalbumin from bovine milk whey by affinity chromatography

Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione, With this material, copper ions were adsorbed from aqueous solutions, the chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g(-1) of organic groups attached to the support and 0.63 mmol g(-1) of copper, This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey, the results showed an efficient separation in the chromatographic column, the possibility of reutilization of the stationary phase was also investigated. (C) 1997 Academic Press

Urinary bladder washings in autopsies. A cytopathologic study of 63 cases

The sediment from urinary bladder washings from 63 consecutive autopsies was cytologically studied in order to achieve a better understanding of the changes in urothelial cells collected from hospital populations. The observed alterations were correlated with alterations in the urinary system and with therapy preceding death. The specimens obtained were of good quality. In 39.7% of the cases, the sediment contained giant superficial multinucleated cells. Three of nine cases previously subjected to radiation or chemotherapy showed atypical urothelial cells. In three cases with immunosuppression, there was cytologic evidence of subclinical infection by polyomavirus, and virus particles were identified by electron microscopy of the vesical mucosa. The study of the smear background offered additional information: the sediment contained hyaline or hematic or hyaline-cellular casts in 17.4% of the cases, in all of which there were renal tubulopathies when the kidney sections were studied. The method is useful for a good evaluation of the autopsy as well as for training in urinary cytopathology.

Use of skin graft punch graft type for the healing of leg ulcers in treated Hansen's disease patients

Hansen's disease is an infectious illness caused by Mycobacterium leprae. It affects preferentially the skin and the peripheral nervous system leading to incapacities, such as leg ulcers, which happens due to the direct action of the bacillus on the organs or its indirect action on the peripheral nervous system. Leg ulcers can occur by two physiopathologic processes. There are many treatments for general leg ulcers, which include the ones caused by Hansen's disease sequels. Among them, surgical treatment shows to be effective when using skin graft, which can be performed by several techniques. Considering the low number of techniques known for treating leg ulcers in Hansen's disease sequels, the aims of this work were to standardize alternative techniques, to detect the main bacteria found in ulcer secretion cultures, to analyze the patients profile and the ulcers, to describe the histophatologies found, and to correlate these data with those of literature from all over the world. Skin graft punch type was carried out and analyzed; males had a mean age of 59.4 years old and females, 54.2 years old. Patients were 73.6% male and 26.3% female. Lepromatous type was present in 89.4% patients and tuberculoid type was seen in 10.5% of them. Associated systemic diseases were observed in 26.3% patients. Mean time of ulcers evolution was 11.6 years in male and 12.8 years in women. The average diameter of ulcers in the pre-treatment period was 8.5 X 9.5 cm in male and 10.2 X 6.8 cm in women. After the graft, their average diameters were 3.2 X 2.7 cm in male and 5.1 X 5.6 cm in women. Statistical analysis showed that there was no significant correlation between the ulcer diameter and its reduction or not in the post-surgery period (p=0.269732). The mean age of patients whose ulcers diameter did not change or reduced by only 20% was 63.5 years. Using the Spearman's coefficient, it was possible to observe that there was no significant correlation between the patients' age and the ulcers diameter reduction after the skin graft (p=0.222531). Evolution time of ulcers that did not present any satisfactory result in the post-surgery period was 12.1 years. The Spearman's coefficient showed that there was no significant correlation between the ulcers evolution time and the ulcers diameter reduction in the post-surgery period (p=0.191655). Cultures presented 50% of cases with Pseudomonas aeruginosa. Statistical analysis showed there is no correlation between the bacterial types found and the ulcer evolution in the post-surgery period (p=0.697531). The average of the ulcers diameter reduction was 42.4%, and in 26.3% of the patients the lesions disappeared after the skin graft.

In vitro comparisons of casting retention on implant abutments among commercially available and experimental castor oil-containing dental luting agents

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of plasma and/or yeast extract on performance and intestinal morphology of piglets from 7 to 63 days of age

The objective of this study was to evaluate the effect of diets supplemented with plasma and/or yeast extract on performance (daily weight gain [DWG], daily feed intake [DFI] and feed conversion [FC]) and intestinal morphology of piglets from 7 to 63 days of age. A total of 288 piglets aged 7 days and weighing 2.57±0.05 kg were studied. A randomized block design consisting of four experimental diets, six repetitions and 12 piglets per experimental unit was adopted. The pre-starter I (7 to 21 days), pre-starter II (22 to 35 days) and starter I (36 to 49 days) diets were supplemented as follows: control diet (CD): no plasma or yeast extract; plasma (PL) diet: addition of 6%, 4% and 2% plasma; yeast extract (YE) diet: addition of 6%, 4% and 2% yeast extract; plasma + yeast extract (PL+YE) diet: addition of 3%, 2% and 1% plasma and yeast extract each. From 50 to 63 days of age all piglets received the same diet. No difference in performance was observed from 7 to 21 days and from 7 to 28 days of age, whereas DWG was higher from 7 to 35 days in piglets receiving the PL+YE diet (268, 278, 271 and 288 g/day for CD, PL, YE and PL+YE, respectively). From 7 to 49 days and from 7 to 63 days, DWG (330 and 519 g/day, respectively) and DFI (307 and 647 g/day) were higher in animals receiving the PL-YE diet when compared with those consuming CD (DWG: 295 and 486 g/day; DFI: 266 and 594 g/day). No significant differences in intestinal morphology were observed between piglets receiving the different diets. The combination of plasma and yeast extract elevates DWG, but does not affect the intestinal morphology of piglets from 7 to 63 days of age. © 2013 Sociedade Brasileira de Zootecnia.