O papel da religião em Gargantua (1534) de François Rabelais: referências e intertextos bíblicos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Características bioquímicas da carne de bubalinos Mediterrâneo terminados em confinamento e abatidos em diferentes pesos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Soil CO2 emission and its relation to soil properties in sugarcane areas under Slash-and-burn and Green harvest

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cardiovascular effects of desflurane following acute hemorrhage in dogs

Objective: To determine the cardiovascular effects of desflurane in dogs following acute hemorrhage.Design: Experimental study.Animals: Eight mix breed dogs.Interventions: Hemorrhage was induced by withdrawal of blood until mean arterial pressure (MAP) dropped to 60 mmHg in conscious dogs. Blood pressure was maintained at 60 mmHg for 1 hour by further removal or replacement of blood. Desflurane was delivered by facemask until endotracheal intubation could be performed and a desflurane expiratory end-tidal concentration of 10.5 V% was maintained.Measurements and main results: Systolic, diastolic, and mean arterial blood pressure (SAP, DAP and MAP), central venous pressure (CVP), cardiac output (CO), stroke volume (SV), cardiac index (0), systemic vascular resistance (SVR), heart rate (HR), respiratory rate (RR), partial pressure of carbon dioxide in arterial blood (PaCO2), and arterial pH were recorded before and 60 minutes after hemorrhage, and 5, 15, 30, 45 and 60 minutes after intubation. Sixty minutes after hemorrhage, SAP, DAP, MAP, CVP, CO, Cl, SV, PaCO2, and arterial pH decreased, and HR and RR increased when compared with baselines values. Immediately after intubation, MAP and arterial pH decreased, and PaCO2 increased. Fifteen minutes after intubation SAP, DAP, MAP, arterial pH, and SVR decreased. At 30 and 45 minutes, MAP and DAP remained decreased and PaCO2 increased, compared with values measured after hemorrhage. Arterial pH increased after 30 minutes of desflurane administration compared with values measured 5 minutes after intubation.Conclusions: Desflurane induced significant changes in blood pressure and arterial pH when administered to dogs following acute hemorrhage.

Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant

eIF5A is a highly conserved putative eukaryotic translation initiation factor that has been implicated in translation initiation, nucleocytoplasmic transport, mRNA decay, and cell proliferation, but with no precise function assigned so far. We have previously shown that high-copy PKCI suppresses the phenotype of tif51A-1, a temperature-sensitive mutant of eIF5A in S. cerevisiae. Here, in an attempt to further understand how Pkc1 functionally interacts with eIF-5A, it was determined that PKCI suppression of tif51A-1 is independent of the cell integrity MAP kinase cascade. Furthermore, two new suppressor genes, ZDS1 and GIC1, were identified. We demonstrated that ZDS1 and ZDS2 are necessary for PKC1, but not for GIC1 suppression. Moreover, high-copy GIC1 also suppresses the growth defect of a PKCI mutant (stt1), suggesting the existence of a Pkc1-Zds1-Gic1 pathway. Consistent with the function of Gic1 in actin organization, the tif51A-1 strain shows an actin polarity defect that is partially recovered by overexpression of Pkc1 and Zds1 as well as Gic1. Additionally, PCL1 and BNI1, important regulators of yeast cell polarity, also suppress tif51A-1 temperature sensitiviiy Taken together, these data strongly Support the correlated involvement of Pkc1 and eIF5A in establishing actin polarity, which is essential for bud formation and G1/S transition in S. cerevisiae.

The genetics of hypertension modifies the renal cell replication response induced by experimental diabetes

To investigate whether the genetics of hypertension modifies renal cell responses in experimental diabetes, we studied the renal cell replication and its regulation by two cyclin-dependent kinase (Cdk) inhibitors, p27(Kip1) and p21(Cip1), in prehypertensive spontaneously hypertensive rats (SUR) and their genetically normotensive counterparts, Wistar Kyoto (WKY) rats, with and without streptozotocin-induced diabetes. In diabetic SIIR, the number of proliferating glomerular (0.6 +/- 0.3 positive cells/50 glomeruli) and tubulointerstitial (2.8 +/- 0.6 positive tubulointerstitial cells/50 grid fields) cells assessed by the bromodeoxyuridine technique was significantly (P = 0.0002) lower than in control SIIR (13.2 +/- 1.7 and 48.6 +/- 9.7, respectively) and control (14.0 +/- 1.8 and 63.9 +/- 10.6) and diabetic (14.3 +/- 3.5 and 66.4 +/- 11.5) WKY rats. Proliferating cell nuclear antigen, another marker of cell proliferation, was significantly reduced in replicating glomerular (P = 0.0002) and tubulointerstitial (P < 0.0001) cells in diabetic SHR. In freshly isolated glomeruli, the level of p27(Kip1) detected by Western blotting was significantly higher In diabetic SIIR than in nondiabetic SHR (1.52 +/- 0.14 vs. 1.00 +/- 0.10% of control, P = 0.014). The expression of p21(Cip1) in isolated glomeruli did not differ among the groups of rats. In conclusion, the response of renal cell replication to diabetes differs markedly between prehypertensive SIIR and their WKY control rats. The decreased glomerular cell proliferation in prehypertensive diabetic SIIR is at least partly mediated by a higher expression of the Cdk inhibitor p27(Kip1).

Use of monocular and binocular visual cues for postural control in children

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determinação seletiva de tributilestanho na presença de Sn(IV) em amostras ambientais usando HG-ICP OES e Saccharomyces cerevisiae como material sorvente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise quantitativa e qualitativa do nível de conhecimento dos alunos do curso de fisioterapia sobre a atuação do fisioterapeuta em saúde pública

Fisioterapia é a ciência da saúde que estuda, previne e trata os distúrbios cinéticos funcionais em órgãos e sistemas do corpo humano. O objetivo deste estudo é verificar a expectativa dos alunos do primeiro ao quinto semestre de fisioterapia sobre a atuação do fisioterapeuta em saúde pública e a expectativa desses alunos quanto à inserção do profissional de fisioterapia no Programa de Saúde da Família (PSF). Trata-se de estudo realizado na Faculdade Marechal Rondon, com 107 alunos, tendo como critério de exclusão os alunos do sétimo semestre. Foi usado um questionário, contendo onze questões, sendo oito de múltipla escolha e três dissertativas. Os dados passaram por tratamento estatístico, em que foram utilizadas a análise descritiva através do programa Microsoft Excel 2003 e análise do discurso do sujeito coletivo. A respeito da atuação do fisioterapeuta no Sistema Único de Saúde (SUS), 44% dos alunos consideram muito importante, 36% consideraram que o papel do fisioterapeuta no PSF é muito importante, enquanto 24% dos alunos consideram não saber informar quanto à eficácia de seu atendimento. Os alunos consideram importante a atuação do fisioterapeuta no SUS e PSF, mas pouco tem conhecimento sobre a atuação do fisioterapeuta em saúde pública.

Collisions with the Earth: the Moon's contribution

In the present work is analyzed the contribution of the Moon on the collisional process of the Earth with asteroids (NEOs). The dynamical system adopted is the restricted four-body problem Sun-Earth-Moon-particle. Using a simple analytical approach one can verify that, the orbit of an object can be significantly affected by the Moon's gravitational field when their relative velocity is smaller than 5 km/s. Therefore, the present work is based on hypothetical asteroids whose velocities relative to Moon are of the order of 1 km/s. In fact, there are several real objects (NEOs) with such velocities at the point they cross the Earth's orbit. The net results obtained indicate that the Moon helps to avoid collisions (2.6%) more than it contributes to extra collisions (0.6%). (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients

Aim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients.

Natureza apropriada: a exploração dos recursos naturais na capitania de Santa Catarina (1738-1808)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Physical quality of an Oxisol after different periods of management systems

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O acento em tupi antigo

O objetivo deste trabalho é estudar a lírica tupi de Pe. José de Anchieta, discutindo a possibilidade de análise do acento da língua tupi antiga, via métrica adotada pelo autor em seus poemas. De toda a sua obra poética, 18 poemas foram escritos inteiramente em língua tupi, 10 deles servindo como corpus para esta pesquisa. Como aporte teórico para essa análise foi utilizada a Fonologia não-linear - mais precisamente a Teoria Métrica de Hayes (1995) - e um modelo de abordagem baseado em Massini-Cagliari (1995,1999 e aorientações na área) para com o português arcaico. As poesias em enfoque foram observadas em várias versões da obra disponíveis (Anchieta 1954, 1984, 2004). Para a base do estudo tupi, foram utilizadas as gramáticas de Anchieta (1933) e Lemos Barbosa (1956), além de livros e textos de Navarro (2006a, 2006b) e Ayron Rodrigues (1958)