135 resultados para hospedeiros alternativos
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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A incidência das leishmanioses tegumentar e visceral americanas, em especial esta última (LVA), em hospedeiros caninos e humanos, encontra-se em crescente processo de expansão no Estado de São Paulo. Para a vigilância epidemiológica dessas endemias, torna-se fundamental o conhecimento da distribuição e da ecologia das diferentes espécies da fauna flebotomínea vetoras. Assim, a divulgação de novos encontros de seus vetores, sobretudo da Lutzomyia longipalpis, o principal vetor da LVA, é fundamental para apontar novas áreas de risco para a transmissão dessas doenças. Neste estudo, capturas de flebotomíneos foram realizadas em ambiente domiciliar, peridomiciliar e de mata, em diferentes localidades rurais dos municípios de Ipeúna e Itirapina, entre outubro de 2001 e fevereiro de 2004. Foram utilizadas armadilhas luminosas automáticas do tipo CDC, das 18h às 8h, em 14 noites, resultando 420 horas de exposição. Foram capturados 177 flebotomíneos pertencentes a doze espécies. A espécie mais abundante, Nyssomyia neivai, apontada como a principal vetora de LTA no Estado, contribuiu com 85,4% dos espécimes capturados em Ipeúna. O encontro de Lutzomyia longipalpis em uma caverna em Itirapina, aponta para o risco de estabelecimento da LVA na área e a necessidade de mais estudos locais sobre sua ecologia, sobretudo em relação à ocupação de ambientes antrópicos.
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The main objective of this study was to estimate genetic and phenotypic trends in weights at birth (BW), 8 (W8) and 12 months (W12) of age; and also in the daily gains from birth to 8 months (G8) and from birth to 12 months (G12) for the ''Guzera'' cattle herd from the ''Fazenda de Ensino e Pesquisa do Campus de Ilha Solteira'', UNESP, at Selviria, MS, in the Central-West region of Brazil. The data were collected between 1979 and 1984 from 842 calves, sired by 22 sires. Genetic trends were estimated by three alternative procedures, two of them were based on the methodology of repeated use of sires and the other procedure on the differences in the average genetic values of sires in the different years. The phenotypic annual changes estimated were -0.232 kg, -8.278 kg, -0.033 kg, -8.498 kg e -0.023 kg, for BW, W8, G8, W12 and G12, respectively. The estimates of genetic trends, obtained by the three procedures were different, but showed that genetic changes for all analised traits were little.
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The number of studies of genotoxic activity of medicinal plants has been growing alongside with their increasing therapeutic use and scientific interest in proving their effectiveness for a variety of pharmacological purposes. This reflects the fact that many of the plants used by great numbers of people, in spite of their proven pharmacological value, can also cause harmful changes in the DNA. The risks are greater when alternative treatments are applied in an uncontrolled way, without due attention to correct botanical identification, to the part of the plant that should to be used and to the method of preparation and administration. In this review, aspects of the mutagenic activity of some medicinal plants are discussed.
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The animal experimentation in the scientific research has contributed excessively for the development of science and technology, promoting to long of the years the discovery of prophylactic measures and treatments for diseases that attack the humans. Animals of some species have been used in the last times, being the mouse the more intensely used and more deeply known scientifically. The objective of this work was to carry through a bibliographical survey including data of our research group, about the use of mice in the animal experimentation, approaching its general biology, reproduction physiology, creation systems, genetics, habitation, feeding, handling, pain and euthanasia, techniques of risk developed in the experimentation, blood collection, pharmacological and toxicological experiments. Although current trends praise the use of alternative methods (in vitro studies, cells cultures, etc.), the animal models, as the mouse, present as main advantage the supply of information on the organism as a whole, fact that is not obtained with other methods, what still it makes possible its utilization in scientific research.
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Despite efforts to the contrary, some cosmetic products can cause undesirable side effects in the users. These may often be due to individual factors or inappropriate use of the product. Thus, biological assays to assess the safety of a new cosmetic must precede its being placed on the market. Historically, these tests have always been carried out in vivo, in animals, since such tests can be used to evaluate many of the potential risks, such as irritation, allergy or systemic effects; but, recently, some research centers have been adopting in vitro alternatives, in order to replace the animal tests. This review emphasizes the need to employ biological assays to test the safety of cosmetic products, and reviews the main in vivo and in vitro tests used, focusing on the need to develop and use alternatives to the in vivo assays of product safety, so as to offer the consumers the maximum safety with the least possible risk, while ensuring the best conditions of use of the product.
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Cosmetics have been used since ancient times and, recently, their consumption has increased greatly in many countries, including brazil, which is the third largest consumer market in the world. Thus, concern for the safety and efficacy of these products should be heightened, even though these products are rarely related to adverse reactions that damage the health. Brazilian law requires manufacturers to subject their products to safety testing, to assess the possible reactions that could be caused by them (irritation, sensitization, systemic effects). To this end, in general, animals have been used as the experimental model, but this practice is being increasingly controlled, so that the scientific community is looking for alternative tests that do not require experimental in vivo models. Thus, this review aims to describe the main biological assays used to assess the safety of cosmetics, as well as in vitro assays that can replace them.
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Environmental problems caused by synthetic fungicides have increased the search for alternative methods of control of plant diseases. The objective was to evaluate the effect of essential oil of citronella grass, on the fungus Rhizoctonia solani, in different methods of in vitro fungitoxicity. We used a randomized design in a factorial design with four replications, where the factors were composed of four methods for assessing the in vitro fungitoxicity of the essential oil of citronella grass (essential oil diluted in Tween 80 (0.5%) and embedded in the culture medium PDA (potato dextrose agar) still melting, essential oil diluted in Tween 80 (0.5%) and distributed on the surface of the PDA; oil essential diluted in Tween 80 (0.5%) and distributed on filter paper attached to the inner surface of the lid of the Petri dish, pure essential oil and distributed on the surface of the culture medium, and control) and five evaluation periods (2, 4, 6, 8 and 10 days of incubation). Was used 0.25μL mL-1 of citronella oil in all treatments. Of the treatments evaluated the use of pure oil distributed on the surface of the culture medium was more effective in reducing the mycelial diameter in all evaluations. In this method the rate of mycelial growth was 9,02 mm day-1, reaching in last evaluation 79,77 mm.
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This work was done to determine the maximum amount of liquid that the citrus leaves can hold back and compare alternative methods for estimating leaf area with the standard method of integrating electronic image. The alternative methods were leaf mirroring on paper and leaf digitalization and imagine analyzes. The spray retention capacity was evaluated with mitecide sprayed with cyhexatin (Sipcatin 500 CS) plus the treatments: combination of two adjuvants (mineral oil - Assist and vegetable oil - Veget'Oil) and two concentrations (10 and 15 mL of adjuvant L-1). The methods for estimating leaf area assessed do not differ between them. The maximum retention of liquids for the leaf occurred when vegetable oil in the application was used.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Pós-graduação em Agronomia (Agricultura) - FCA
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)