Crescimento e sobrevivência do camarão-rosa (Farfantepenaeus paulensis) na fase de berçário em meio heterotrófico

The objective of the present study was to evaluate the potential of a heterotrophic rearing media (microbial flocs) on the growth and survival of Farfantepenaeus paulensis reared in the nursery phase. For 30 days, F. paulensis post-larvae (0.019 ± 0.01g) were reared in nine 40-liter plastic tanks at the density of 500 post-larvae m -2. Three treatments with three replicates each were used: rearing in the presence of microbial flocs with ration supply (FLOC + R); rearing in the sole presence of flocs - no ration supplied (FLOC); and rearing in clear water plus with ration supply (AC + R). The presence of microbial flocs had no significant effect on growth and survival in the nursery rearing of F. paulensis.

Sistema de reprodução em pequenas populações fragmentadas e em árvores isoladas de Hymenaea stigonocarpa

Six microsatellite loci were used to quantify the mating system of two small fragmented populations (Selviria - SEL and Aparecida do Tabuado - APT, Mato Grosso do Sul State) and isolated trees in pastures, of the bat-pollinated tropical tree Hymenaea stignocarpa, growing in the Center-west region of Brazil. In SEL population, seeds were collected from 11 mother-trees; in APT, from three trees and, in the case of isolated trees, from six individuals growing at least 500 m apart in pastures. To investigate if there are differences on mating system between trees in populations and isolated trees, trees from populations were pooled as a group and, likewise, the isolated trees were pooled to another group. The outcrossing rate was higher in the populations (t̂ m= 0.873) than in isolated trees (t̂ m=0.857), but the difference was not significant. Significant and high differences between multi-locus and single-locus outcrossing rate were detected in populations (t̂ m- t̂ s=0.301, P<0.05) and isolated trees (t̂ m- t̂ s=0.276, P<0.05), suggesting mating between relatives. Higher paternity correlation was observed in trees from population (r̂ p=0.636) than in isolated trees (r̂ p=0.377), indicating the occurrence of some correlated matings and that part of offspring are full-sibs. It was not observed increased in self-fertilization rate in isolated trees in pastures. In general terms, the unique observed difference in mating system between populations and isolate trees was the high rate of correlated matings in trees from populations, due probably to the small distance among coespecifics and the pollinator behavior, visiting near trees.

Alterações de propriedades físicas de um nitossolo vermelho pela sucessão milheto-soja na fase de implantação do sistema de semeadura direta

The aim of this work was to evaluate the effect of a millet-soybean rotation, during the implantation phase of no-tillage system on the physical properties of a Nitossolo Vermelho distroférrico and the dry mass production of millet and the productivity of soybean. The experimental design used was a randomized blocks, in a split splot arrangement, with four replications. The parcels was constituted by three sowings (E1, E2 and E3) and the subparcels was constituted by harvests, where each harvest handling was: M1 - harvest each blooming and covering withdrawal; M2 - harvest each blooming and covering permanency; M3 - only in the first harvest on blooming and covering withdrawal; M4 - only in the first harvest on blooming and covering permanency, and M5-free growing, with no harvest. Samples were collected from three soil layers: 0-0,05, 0,05-0,10 and 0,10-0,20 m. The E2 showed smaller values of soil density and larger values of total porosity. The E3 resulted in smaller values of flocculation degree and mean weight diameter of the aggregates. The E3 showed smaller production of dry mass.

Fitase em rações para tilápia-do-nilo na fase de crescimento

This research was carried out to compare the effect of phytase on performance, in the incorporation and excretion phosphorus in Nile tilápia. Four diets were used: one standard (contol), composed by 3,200 DE kcal ED/kg of ration and 30.0% CP and supplemented with phosphorus (4.0% of dicalcium phosphate), considering the treatment control, and others three, without phosphorus supplementation, with different phytase levels (1,000, 1,500, and 2,000 uf/kg), with four replication, per a period of 82 days. Circular tanks of fiber glass, with constant flow and aeration were used. The results demonstrated that the use of phytase in diets for fish production can reduce the levels of inorganic phosphorus in the diets and minimizing the impacts caused by phosphorus in the production system and in the natural environment and also can improve the productivity, increasing the amount of feed to be supplied and, consequently, facilitating the increase of biomass of fish to be used per production area. © 2007 Sociedade Brasileira de Zootecnia.

Um método de gestão de fornecedores baseado na avaliação da eficácia de auditorias realizadas na fase de desenvolvimento de produtos

The main goal of this work is to present a supplier's management method based on evaluation of audits performed at suppliers during products development phase. Audits are prioritized according to the impact of supplied parts on the development and its main goals are identification of risks, to propose and perform action plans and to evaluate periodically the supplier's performance to prevent problems. It is proposed that if the audits in the development phase be efficient, the supplied products will have good maturity to entry into service and stable performance. This issue was evaluated in a aeronautic company and the analyzed results in 2006 were considered good.

Perfil eletroforético das proteínas de fase aguda em caprinos experimentalmente infectados com Trypanosoma evansi

Considered as one of the main agents of the tripanossomiases, Trypanosoma evansi causes a disease generically know as surra, with wide geographic occurence. This work has the aim to study the electrophoretic profile of the acute phase proteins of goats, experimentally infected with T. evansi. Ten crossbread female goats, around 4 months of age, clinically healthy and serum negative for the presence of antibodies anti-T. evansi (IFAT) were used. The animals were divided in two groups: six were inoculated (G1) intravenously with 2,38 × 10 6 tripomastigotes of T. evansi and four were kept as noninfected controls. The blood for serum was collected daily until the 14 days after inoculation (DAI), weekly up to the 98 th DAI and every two weeks up to the 364 th DAI. The serum proteins were separacted by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE). Twenty-one proteins were found in the serum of the goats, eight were nominally identified; phosphorylase, transferrin, albumin, antitrypsin, acid glicoprotein, haptoglobin, hemoglobin, and light chain immunoglobulin.

Efeito da temperatura e tempo de austenitização nas transformações de fase da liga 13Cr2Ni0,1C

New classes of martensitic stainless steels, with low carbon levels, have been developed aiming to meet the needs of the petroleum industry segment. However, their use has been restricted due to the fact it is a recent development and many of its properties are still under investigation. This work determines the values of initial and final temperatures for the austenitic transformation and the initial and final temperatures of martensitic formation for alloy 13Cr2Ni0,1C, by means of dilatometric tests under continuous cooling. Based on these results the optimized conditions for quench and temper heat treatments were obtained. The microstructural characterization of the alloys under coarse fusion condition was carried out by optical microscopy and the presence of delta-ferrite in the martensitic matrix was observed.

Detecção de Escherichia coli e Salmonella spp. em microbiota intestinal de Psittaciformes em fase de reabilitação para soltura

Escherichia coli is a bacteria of the Enterobacteriacea family and it is part of the enterical microflora of mammals and of many species of birds. Salmonella spp. also belongs to the family Enterobacteriacea, it is responsible for human feed toxinfection outbreaks and usually isolated from domestic and wild birds. The present study analyzed the frequency of both agents in Psittaciformes in rehabilitation process for wildlife reintroduction. In 89 birds analyzed, 19% were infected with E. coli and 1,12% with Salmonella spp. It was carried out an analysis of the profile of antibiotic resistance in which was observed the efficiency of estreptomicin, tetraciclin, trimetoprim and gentamicin over the samples. The samples of E. coli were submitted to the Congo Red Binding test and to the Hemolisis test and 70,6% of positive samples for the first test and 53% for the second one were observed.

Avaliação das dimensões transversas na maxila e mandíbula em pacientes na fase da dentição mista tratados com aparelho ortodôntico removível superior

Objeti vo: Avaliar os efeitos da expansão lenta na maxila e na mandíbula com o aparelho ortodônti co removível superior com torno de expansão simétrico e mediano, nas regiões oclusal, gengival e alveolar de pacientes jovens com atresia maxilar. Método: A amostra compreendeu 18 indivíduos leucodermas (11 meninas e 7 meninos; idade média: 8 anos e 10 meses no início do tratamento) que apresentavam atresia da maxila, acompanhadas ou não de mordida cruzada posterior uni ou bilateral na fase de denti ção mista. Todos os pacientes foram tratados com aparelho ortodônti co removível superior com torno de expansão simétrico e mediano, sendo o tempo médio de tratamento de 15,4 meses (± 7,6). Para avaliar a infl uência do tratamento nas mensurações dos pontos demarcados nas regiões oclusal, gengival e alveolar, foram uti lizados os modelos de gesso dos arcos superior e inferior (36 pares) obti dos em dois tempos: T1: início do tratamento e T2: ao fi nal do tratamento. Para cada paciente em ambos os tempos, foram mensuradas as distâncias transversas na região oclusal entre caninos decíduos, 1o molares decíduos ou 1o pré-molares permanentes e entre 1o molares permanentes, superiores e inferiores. Para verifi car se a movimentação ocorreu por inclinação ou por movimento de corpo, foram uti lizadas também medidas nas regiões gengival e alveolar. Os dados foram avaliados estati sti camente pelo teste “t student para amostras pareadas (5%). Resultados: Observou-se que em T2, todas as distâncias mensuradas para as regiões oclusal, gengival e alveolar apresentaram valores estati sti camente superiores às mesmas medidas em T1 (p<0,05). Conclusão: O aparelho ortodônti co removível superior é efeti vo nos casos de expansão lenta da maxila, agindo também indiretamente nas dimensões transversas do arco inferior.

Plasma suíno e ovo inteiro em rações de leitões sobre desempenho na fase inicial e efeito residual até a terminação

Were used 64 weaned pigs, distributed in eight treatments: ration with skim milk (SM), three rations with crescent levels of swine plasma (SP), three rations with whole egg (WE) and a ration with high inclusion of soybean meal (SB). Daily weight gain (DWG), daily feed intake (DFI) and feed conversion (FC) were evaluated. The animals that consumed ration SB have the mean of DWG higher that other treatments, and inferior FC, from 35 to 48 days of age. The crescent levels of SP promoted linear reduction in DWG, from 21 to 35, and from 35 to 48 days of age, in DFI from 48 to 138 days of age and linear increased in FC from 21 to 35 of age. Considering the weaned phase until finishing, initial rations can be formulated with SP and WE substituted partially the CP of skim milk in 25 and 45%, respectively.

Simbiótico e extratos naturais na dieta de codornas Japonesas na fase de postura

The objective of this study was to assess the performance, tonic immobility time (TIT), intensity of injuries (II) and heterophil to lymphocyte ratio (H:L) by the addition of the symbiotic and plant extract (Aloe vera and Symphytum officinale) to the diet of Japanese quails. Ninety quails were used, distributed in randomized blocks with 3 treatments (0-control; 250 and 750 mg symbiotic and plant extract/kg of diet), five repetitions and six birds per cage. Feed intake, feed conversion, production and weight of eggs, viability, TIT, II and H:L were evaluated. Results showed that the addition of the product to the diet did not affect the performance, however, it decreased the TIT, II and the H:L of quails which received the highest level of the product in the diet. Thus, the use of symbiotic and plant extract in the diet has been promising regarding the behavioral and physiological parameters, decreasing the stress of the animals, mainly for the level of 750 mg/kg diet.

Acurácia da histerossonografia versus ultrassonografia transvaginal em mulheres inférteis candidatas às técnicas de reprodução assistida

PURPOSE: To compare the diagnostic accuracy of sonohysterography (HSN) and conventional transvaginal ultrasound (USG) in assessing the uterine cavity of infertile women candidate to assisted reproduction techniques (ART). METHODS: Comparative cross-sectional study with 120 infertile women candidate to ART, assisted at Centro de Reprodução Assistida (CRA) of Hospital Regional da Asa Sul (HRAS), Brasília-DF, from August 2009 to November 2010. Sonohysterography was performed with saline solution infusion in a close system. The sonohysterography finding was compared to previous USG results. The uterine cavity was considered abnormal whenthe endometrium was found to be thicker than expectedduring the menstrual cycle and whenan endometrial polyp, a submucous myoma and an abnormal shape of the uterine cavity were observed. The statistical analysis was doneusing absolute frequencies, percentage values and the χ 2, with the level of significance set at5%. RESULTS:HSN revealed that 92 (76.7%) infertile women candidate to ARThad anormal uterine cavity, while 28 (23.3%) had the following abnormalities: 15 polyps (12.5%).

Sistema de reprodução, parentesco e tamanho efetivo em sementes de polinização livre de populações fragmentadas de Copaifera langsdorffii Desf. por análise de locos microssatélites

Anthropogenic changes in the reproductive population density can affect the mating system and result in an increase in selfing and correlated matings. This study investigated the mating system in small fragmented populations of the insect pollinated tropical tree species Copaifera langsdorffii, using microsatellite loci and the mixed and correlated mating models. Open-pollinated seeds were collected from 15 seed-trees located in a small forest fragment (4.8 ha), denominated Bosque and from 14 other seed-trees located in other small forest fragments of the north-western region of São Paulo State. No significant differences were observed between the seed-trees from Bosque (tm=0.933±0.028) and other fragments (tm=0.971±0.032), although these estimates were significantly different from 1.0, suggesting that selfing was occurring. Differences between multilocus and unilocus outcrossing rate were significantly high in both seed-trees of Bosque (tm -ts=0.478±0.05) and other forest fragments (tm -ts=0.475±0.018), suggesting a spatial genetic structure in those stands. The results also showed high rates of correlated mating in the samples, indicating that a good part of the offspring were full-sibs. As a consequence of selfing, mating among relatives and correlated matings, the coancestry within families was equally high in the seed-trees of Bosque ((Θ=0.237) and in the seed-trees of the other forest fragments (Θ=0.241) and the effective population size was lower than expected in panmitic populations (Ne<4). The results were discussed, focusing on the sample size of seed-trees to collect seeds for genetic conservation and enviromental reforestation.

Agonistas do GnRH, antagonistas do GnRH e a reprodução assistida: Análogos do GnRH x fertilização in vitro

The agonists of gonadotropin-releasing hormone (GnRH) were introduced in ovarian stimulation for in vitro fertilization to avoid a premature surge of luteinizing hormone. Although they are accompanied by some disadvantages, GnRH agonists have become well accepted in clinical practice, and their use is associated with increased rates of pregnancy. The development of GnRH antagonists capable of blocking the pituitary immediately offered a therapeutic option. Comparative studies between the two analogs have suggested that the use of antagonists is associated with a shorter duration of ovulatory stimulus and a decreased incidence of ovarian hyperstimulation syndrome, while the type of GnRH analogues used show no significant effects on the rates of pregnancy and live birth. However, GnRH agonists have other applications in assisted reproductive technology cycles than the pituitary downregulation.

Digestibilidade da soja semi-integral extrusada para leitões na fase inicial

Two assays were conducted to determine the coefficients of digestibility (CD) and the digestible nutrients (DN) of the extruded semi-whole soybean (Assay 1 conventional feeding and Assay 2 feeding for sows, starting at 109 days of pregnancy and during the lactation and for lactating piglets and in the initial phase, with rations without soybean meal). Each assay was divided into two phases (32 and 50 days of age). Eight piglets weaned at 25 days of age were used. Total feces collection method was used. The following variables were analyzed in feed and in feces: dry matter, crude protein, ether extract, crude fiber and gross energy. The following average values were obtained: CDDM 88.87%; CDCP 86.81%; CDEE 85.41%; CDCF 78.22%; CDGE 80.21% and CMDE 95.71%, which allowed the calculation of the digestible nutrients: DDM 81.28%; DP 34.49%; DEE 14.02%; DF 4.49%; DEa 4087.86 kcal/kg and MEa 3912.73 kcal/kg. The different coefficients of digestibility were not affected by the differential feeding for sows and piglets nor by the different ages of the animals. Moreover, the extruded semi-whole soybean presented good levels of DN, concerning mainly the digestible and metabolizable energy.