Efeito de diferentes técnicas de ventilação mecânica sobre o estresse oxidativo pulmonar induzido por lesão pulmonar aguda experimental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estresse oxidativo em pacientes beta talassêmicos heterozigotos e com deficiência de glicose-6-fosfato desidrogenase

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Processo oxidativo em doadores de sangue portadores de hemoglobina S e mutantes no gene HFE

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação nutricional de estresse oxidativo e ocorrência de infecção em indivíduos institucionalizados do Asilo Padre Euclides de Botucatu-SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de programa para mudança do estilo de vida sobre indicadores sanguíneos dos estresses inflamatórios e oxidativo em adultos portadores de componentes da síndrome metabólica

Pós-graduação em Patologia - FMB

Prevenção do estresse oxidativo pela utilização de trolox®, catalase e glutationa no processo de congelação de sêmen ovino

Pós-graduação em Medicina Veterinária - FMVZ

Association between oxidative stress and nutritional status in the elderly

O envelhecimento é um processo dinâmico e progressivo que se caracteriza pela ocorrência de modificações morfológicas, bioquímicas, funcionais e psicológicas do organismo. O objetivo do presente artigo é fornecer conceitos atualizados sobre estresse oxidativo, abordando sua importância no envelhecimento, assim como o estado nutricional e suplementação com antioxidantes (substâncias que evitam ou atenuam a oxidação de substratos oxidáveis, como lipídeos, proteínas, carboidratos e o ácido desoxirribonucleico) na população geriátrica. Evidências sugerem que existe uma relação inversa entre estresse oxidativo e estado nutricional em indivíduos idosos. Embora seja comprovado o aumento do estresse oxidativo nas doenças crônicas associadas ao envelhecimento, como doença de Parkinson e doença de Alzheimer, não há, até o momento, evidências clínicas consistentes que comprovem a eficiência da suplementação com antioxidantes contra o estresse oxidativo. Nesse contexto, a suplementação não é recomendada. Por outro lado, idosos devem ser encorajados a ingerir alimentos antioxidantes, como, por exemplo, frutas e vegetais. A manutenção do peso dentro da faixa de normalidade (índice de massa corpórea entre 23 e 28 kg/m2) também deve ser estimulada.

Efeito do betacaroteno sobre o estresse oxidativo e a expressão de conexina 43 cardíaca

BACKGROUND: Intervention studies have shown an increased mortality in patients who received beta-carotene. However, the mechanisms involved in this phenomenon are still unknown. OBJECTIVE: Evaluate the influence of beta-carotene on oxidative stress and the expression of connexin 43 in rat hearts. METHODS: Wistar rats, weighing approximately 100 g, were allocated in two groups: Control Group (n=30), that received the diet routinely used in our laboratory, and Beta-Carotene Group (n = 28), which received beta-carotene (in crystal form, added and mixed to the diet) at a dose of 500 mg of beta-carotene/kg of diet. The animals received the treatment until they reached 200-250g, when they were sacrificed. Samples of blood, liver and heart were collected to perform Western blotting and immunohistochemistry for connexin 43; morphometric studies, dosages of beta-carotene by high-performance liquid chromatography as well as reduced glutathione, oxidized glutathione and lipids hydroperoxides were performed by biochemical analysis. RESULTS: Beta-carotene was detected only in the liver of Beta-Carotene Group animals (288 ± 94.7 µg/kg). Levels of reduced/oxidized glutathione were higher in the liver and heart of Beta-Carotene Group animals (liver - Control Group: 42.60 ± 1.62; liver - Beta-Carotene Group: 57.40 ± 5.90; p = 0.04; heart: - Control Group: 117.40 ± 1.01; heart - Beta-Carotene Group: 121.81 ± 1.32 nmol/mg protein; p = 0.03). The content of total connexin 43 was larger in Beta-Carotene Group. CONCLUSION: Beta-carotene demonstrated a positive effect, characterized by the increase of intercellular communication and improvement of anti-oxidizing defense system. In this model, mechanism does not explain the increased mortality rate observed with the beta-carotene supplementation in clinical studies. (Arq Bras Cardiol. 2013; [online].ahead print, PP.0-0)

Metabolismo oxidativo e cardíaco de equinos submetidos a exercício de baixa intensidade antes e após suplementação com antioxidante

The present study aimed to evaluate cardiac and lipoperoxidation markers in horses subjected to low intensity and long duration (TLD) exercise test, before and after vitamin E supplementation. For this purpose, 10 horses were used, subjecting them to the first TLD with a workload based on individual maximal oxygen uptake (VO2max). Then, horses received vitamin E (dl-alpha-tocopherol) during 59 days at a daily oral dose of 1,000IU, and thereafter they performed a second TLD with the same protocol as the first. Blood samples were collected to determine plasma malondialdehyde (MDA) as an index of lipoperoxidation, serum cardiac troponin I (cTnI) and creatine kinase MB isoenzyme (CK-MB) as cardiac markers. As a result of the exercise, there was no significant increase in MDA or cTnI, but serum CK-MB increased suggesting myocardial stress. The supplementation was able to minimize reactive oxygen species production, as evidenced by lower concentrations of MDA at all times evaluated, but it didn't cause protective effect on the myocardium. It was concluded that the low intensity and long duration exercise promoted light myocardial stress in horses and vitamin E supplementation reduced lipoperoxidation.

Avaliação dos efeitos deletérios do diuron e de seus metabólitos em lambaris (Astyanax sp.): testes de toxicidade, marcadores de estresse oxidativo, marcadores genotóxicos e enzimas de biotransformação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estresse oxidativo, alterações metabólicas e renais decorrentes da elevada ingestão de sacarose: efeito protetor da N-acetilcisteína (NAC)

Oxidative stress is related with physiopathology of diabetes mellitus type II and with its secondary complications, such as diabetic nephropathy. Thus, the purpose of this study was to examine the effects of n-acetylcysteine NAC, an antioxidant from Allium cepa, on oxidative stress, morphometrical and nutritional parameters and basal metabolism and energetic substrate utilization, serum glucose and oral glucose tolerance test (OGTT), and renal function of high-sucrose intake rats. Animals were initially divided into three groups. Rats in the control group (C; n=10) were given free access to a standard chow and water; (S; n=10) group received standard chow and 30% sucrose in its drinking water; (SN; n=5) group received standard chow and 2g/L NAC and 30% sucrose in its drinking water. After 25 days of treatments, rats were submitted to indirect calorimetry in fasted and feed states. After 30 days of treatments, rats from C and S groups were divided into four groups of five rats each. The (CC) and (SS) groups were given an intra-gastric dose 0,6mL saline (0,9%NaCl) and the (CNAC) and (SNAC) groups were treated with a intra-gastric dose of 0,6mL NAC (160g/day), and then everybody received a intra-gastric dose of glucose solution (20%) for the OGTT. Animals of S and SN groups have higher liquid consumption and lower food consumption than C group. Calorimetric analyses confirm that despite of the final body weight had not statistical difference among groups, S group have lower resting metabolic rate when compared to C and SN animals. Besides, S group has higher respiratory quotient, higher carbohydrate oxidation and lower lipid oxidation, both in fasted and feed states, than C and SN groups, evidencing the beneficial effect of NAC. Fasting plasma glucose is increased in SS and SNAC animals when compared to CC and CNAC, however SN group has glicemic level at 30 min before OGTT decreased when ...(Complete abstract click electronic access below)

Estresse oxidativo em cães com doença renal crônica

Pós-graduação em Medicina Veterinária - FCAV

Avaliação de fatores preditivos de estresse oxidativo em pessoas saudáveis

Evaluation of predictive factors for oxidative stress in healthy people. This study aimed to evaluate the inÀuence of factors such as smoNing, alcohol consumption, use of medicines, contact Zith chemicals, as Zell as age and gender on marNers of oxidative stress, in healthy subMects. :e checNed the effect of age on the antioxidant capacity, Zith decreased values in older individuals, Zhich may be the cause of increased oxidative stress, associated Zith aging. Regarding the other factors, no differences Zere found in the values of the marNer of lipid peroxidation and the antioxidant capacity.

Efeito da suplementação com vitamina E sobre os metabolismos oxidativo e cardíaco em equinos submetidos a exercício de alta intensidade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)